Thesis (M.A.) -- Central Connecticut State University, 2006. / Thesis advisor: Kathy Martin. "... in partial fulfillment of the requirements for the degree of Master of Arts in Biomolecular Sciences." Includes bibliographical references (leaves 99-103). Also available via the World Wide Web.

Thesis (MMedVet (Medicine))--University of Pretoria, 2006. / Includes bibliographical references.

The occurrence of tick-borne pathogens in dogs on communal land in Botswana was studied using Reverse Line Blot (RLB). Eighty blood samples were collected from dogs that underwent a spay and neuter program in Maun, Botswana. These were sent to the Department of Veterinary Tropical Diseases, University of Pretoria, and were stored at the Molecular Laboratory. DNA was extracted from the samples followed by the Polymerase Chain Reaction (PCR) and the RLB. Six samples were found to be positive for Ehrlichia canis. Two of the samples were positive for a concurrent infection of E. canis and Ehrlichia ruminantium. There was one sample that was positive for the non-pathogenic Ehrlichia sp. Omatjenne. Although six samples were positive for the Babesia/Theileria catch-all probe, none hybridised with any of the species-specific probes. Copyright / Dissertation (MSc)--University of Pretoria, 2011. / Veterinary Tropical Diseases / unrestricted

Two studies were conducted to evaluate the presence of tick-borne bacterial agents in Amblyomma americanum, lone star tick (LST), and various wildlife in Mississippi. Adult LSTs had DNA evidence of Ehrlichia chaffeensis (3.7%), E. ewingii (6.3%), and Borrelia lonestari (2.6%), while both larval (24.3%) and adult (43.5%) LSTs were positive for a Rickettsia spp. by polymerase chain reaction (PCR). White-tailed deer (WTD) were the only wildlife PCR positive for E. chaffeensis (18.8%), B. lonestari (3.1%), and Anaplasma phagocytophilum (3.1%). In addition, WTD had the highest seroprevalence to B. lonestari (19.3%) and E. chaffeensis (43.9%) antigens while raccoons had the highest seroprevalence to spotted fever group rickettsiae (R. parkeri antigen) (73.7%). These studies demonstrate evidence of potentially zoonotic tick-borne agents in LSTs and wildlife in Mississippi underscoring the importance of monitoring these agents for human and animal health.

No description available.

<p>More than 15 tick-borne diseases have been recognized in the United States (US) (Eisen (2020). Collectively, TBDs have been linked to seven different tick species in the US. Climate change, urbanization, and host migration exacerbate the threat of increasing TBD incidence and the expanding geographic distribution of ticks. Furthermore, data suggests that less than a tenth of the reportable TBDs were reported to the CDC annually. Studies from a national survey and high incidence states for Lyme disease provided evidence that healthcare providers have knowledge gaps about TBD epidemiology and diagnostics, which has yet to be examined in Indiana to our knowledge.</p><p></p><p>An anonymous survey of 597 Indiana healthcare providers was conducted between February 28^th and April 10^th,2020 to assess knowledge about ticks and tick-borne diseases (TBDs). The survey comprised 22 discrete and ordinal questions and one open-ended question. The study revealed that 92% of the respondents reported Doctor of Medicine (MD) (71%), Doctor of Osteopathic Medicine (DO) (8%), Physician Assistant (PA) (12%) and Registered nurse (RN) (1%) as their highest level of training and practiced in a diversity of settings including Primary care office (28%), Specialty care office (23%) and Hospital (inpatient) (20%). Analyses revealed that 39% of respondents had practiced for more than 25 years, consulted between 1-25 patients with confirmed or suspected TBD per year, and predominantly served both rural and urban communities (62% respondents). Seventy percent of respondents recognized the clinical signs and symptoms of TBDs endemic to Indiana and 96% of DOs, 94% of PAs, and 91% of MDs were aware of the limitations of the erythema migrans (EM) rash as a diagnostic criteria for Lyme disease (LD), the most common TBD in Indiana and the U.S. The majority of respondents (75%) reported using fine tip tweezers, considered best practice for the removal of an attached tick. Among respondents who used online resources to obtain information regarding diagnostic and treatment guidelines, 25-45% visited federal (CDC) and state (Indiana Department of Health, IDOH) websites. </p><p>Survey data revealed several potential knowledge gaps among Indiana state providers. Respondents reported that they were unsure about the following topics: (1) the tick species capable of disease transmission in IN (47% unsure), (2) the geographic distribution of the black legged tick, <i>Ixodes scapularis, </i>the vector of LD in the U.S. (56% unsure), (3) northwest Indiana as the region of the state associated with highest LD risk (49% unsure), and (4) May-July as the months considered highest risk for TBD transmission (48% unsure or answered incorrectly). These findings provided evidence to support the hypothesis under investigation in the present study that Indiana healthcare providers lack knowledge of tick vectors and TBDs risks specific to the state. </p>Qualitative analysis was conducted for open-ended question (Q23) and resulted in coding of 597 responses into six major categories (Healthcare provider, Healthcare system, Patient Knowledge, Disagreement, Education and Not applicable) and 15 associated subcategories. Coding analyses revealed that healthcare providers are aware of their shortcomings and are receptive to online interventions. Here we present the first focused study to assess provider knowledge of ticks and TBD in Indiana. Taken together, the survey findings provide information to guide development of region-specific educational material and guidelines about ticks and TBDs for healthcare providers. We provide evidence to suggest that a continued provider education program may improve diagnosis and treatment of TBDs.

No description available.

Tick-borne encephalitis risk assessment based on classification of vegetation from remote sensing data Abstract The main aim of this thesis has been to find out how to classify various categories of forest vegetation with a different risk of exposure to the tick-borne encephalitis based on the Landsat imagery. The legend used here is derived from the one used in the projects by Daniel, Kolář, Zeman (1995) and Daniel, Kolář, Beneš (1999) but has been reduced to only five classses with no overlaps in their definitions (I. coniferous stands, II. mixed stands, III. young deciduous stands and stand ecotones with a highly heterogeneous structure, IV. deciduous stands with a homogeneous structure, V. deciduous stands with a heterogeneous structure). The supervised classification with the Maximum Likelihood Classifier has been used on the Landsat imagery from various seasons. Difficulties concerned with the presence of clouds and varying Sun elevation across the imagery had to be adressed in the course of the work. The training sites and the control points have been defined by the field research and interpretation of the relevant orthophotomaps and Landsat imagery in 5-4-3 RGB composite. The mask of the forest has been created on the ZABAGED data basis. The time horizon of 2006 - 2010 has been the primary focus....

Babesiosis is a disease caused by parasites of Babesia species that is spread through ticks. Babesiosis can affect humans and many other mammals all over the world. In the United States, babesiosis is mainly caused by Babesia microti with additional species of Babesia infecting dogs. Dogs have long been known to be a good indicator species for human tick-borne infection due to the shared spaces they have with humans and their tendency to explore and pick up diseases in the environment. This study used a group of hunting dogs to determine the presence of Babesia infection. The goal was to determine a predictive model for human infection. Infection was defined as having a positive antibody test or molecular test for Babesia species in blood samples. Blood samples were taken at two time points, tested, and compared. Statistical methods were used to analyze the results of the tests and compare them with variables such as region, gender, age, and other diseases the dogs were exposed to. Of 214 dogs, 56 had Babesia infections, with a prevalence rate of 26.2%. At the first time point the model showed age and infection with Anaplasma platys as being significant. At the 2nd time point, 29 dogs were lost to follow up, leaving a total of 185 dogs sampled with 63 testing positive for Babesia spp. The rate for the 2nd time point was 34.1%. No variables were found to be significant in the model for the 2nd time point. Despite differences in Babesia that infect humans and dogs, the hunting dog cohort reflects a model that validates the environmental exposures, coinfections, and demographic variables that affect transmission of the pathogen. Furthermore, findings of this study cast doubt on the ability of Ixodes scapularis to act as capable vector for canine-infecting Babesia species.

Understanding the ecology and epidemiology of tick-borne diseases requires detailed knowledge of the complex interactions among the tick vector, the microorganisms they carry and the vertebrate hosts used by ticks, as well as the environmental conditions experienced by all three groups of organisms in this triad. In this thesis, I addressed questions relating to the biology and vector ecology of the Rocky Mountain wood tick (Dermacentor andersoni) and the American dog tick (Dermacentor variabilis). Comparisons were made of the distribution of both tick species, the vertebrate hosts used by immature ticks, and the types and prevalence of bacteria in individual ticks from multiple localities near the northern extent of their geographic ranges in western Canada. The results revealed that the distributions of both D. andersoni and D. variabilis have expanded since the 1960s, and there is now a broad zone of sympatry in southern Saskatchewan. In this zone of sympatry, D. andersoni and D. variabilis immatures were found to use the same species of small mammals as hosts and, in some cases, the same host individuals. This provides for the possibility of cross-transmission of bacteria from one tick species to the other. Bacteria of several genera (e.g. Rickettsia, Francisella, Arsenophonus and Anaplasma) were detected in D. andersoni and/or D. variabilis, some of which represented new tick-bacteria associations. However, most bacterial species were highly host (tick)-specific, except for three examples of apparent host switching from one tick species to the other at localities where the two tick species occurred in sympatry. The findings of this thesis provide a basis for understanding microbial transmission, the structure of tick-borne microbial communities, the risk of tick-borne disease in humans and animals, and the vector potential of D. andersoni and D. variabilis in geographical areas where they have not been studied previously.