Thesis advisor: Noah P. Snyder / Nitrogen is an important component in the biogeochemical processes of freshwater systems. Likewise, it is unknown if, and to what magnitude, changes in land use in the watersheds of New England lakes have affected nitrogen availability. This study examines the effects of land-use change on the present and historic isotopic signatures of nitrogen in three New England lakes of varied histories, Lower South Branch Pond, Little Kennebago Lake, and Sennebec Pond. The histories of all three sites indicate minimal discernible disturbance before the onset of Euro-American-induced land use change. For two sites, the dominant mechanism of change was timber harvest, which began in the latter half of the 19th century. Sediment cores for each site were examined and variations in geochemical and sedimentological indicators were evaluated in the context of changes within respective basins. Statistical analysis indicates significant shifts in the means and variance of the geochemistry within the Little Kennebago Lake and Sennebec Pond watersheds after the incursion of Euro-American settlers, while the Lower South Branch Pond watershed displays similarities to a more widespread signal of anthropogenic nitrogen that has been deposited remotely. The record of magnetic susceptibility in Little Kennebago Lake displays the largest variation compared to the other two lakes, which may indicate that the magnitude of land-use change within the basin was more impactful relative to Lower South Branch Pond and Sennebec Pond. This is significant in that all three sites experienced some level of land-clearance. / Thesis (MS) — Boston College, 2023. / Submitted to: Boston College. Graduate School of Arts and Sciences. / Discipline: Earth and Environmental Sciences.

The overall goal of this project was to provide baseline data on amphibian species richness, relative abundance, and habitat use for a long-term landscape ecology study on MeadWestvaco industrial forest in the Allegheny Highlands of West Virginia. From results of area-constrained daytime searches (10 m x 10 m plots) across the landscape, I developed 9 regression models to predict amphibian relative abundance. I constructed models for each year for all plots on all habitat types, plots that were in a Stream Management Zone (SMZ), and plots that were in upland, or non-SMZ, habitat. Distance to perennial or ephemeral streams or perennial ponds (SMZ classification), the amount of available rocks along transects, and site index were the 3 most important habitat variables in models for all plots combined and were responsible for 24-32% of the inherent variation in population relative abundance. Other habitat variables that were significant in models were year, % canopy cover, the amount of available woody debris of decomposition classes 3-5 along transects, % woody stems (<7.5 cm DBH), soil pH, and % herbaceous vegetation. R2PRESS values for all 9 models ranged from 0.08 to 0.35. Amphibian relative abundance showed positive relationships with all significant habitat variables with the exception of year and % woody stems. In natural cover object use/availability analyses, I discovered salamanders preferred rocks over woody debris, relative to the amount available of each. Salamanders preferred flat rocks to any other shape, flagstones to any other type of rock, and rock lengths in the 31-40 cm class. Preferred wood widths were in class 5-10 cm, while preferred wood lengths were in class <50 cm; salamanders exhibited strong preferences for wood in higher states of decomposition (class 3-5). I provided baseline, preharvest data for 28-acre reference areas on 9 forest compartments scheduled for clearcuts. I sampled all 9 reference areas preharvest and sampled 3 during year 1 postharvest using coverboard and night plot surveys. On these 3 areas, species richness declined from preharvest to postharvest, but species diversity showed little change. Overall relative abundance declined significantly preharvest to postharvest with coverboard sampling (p=0.0172) and night plot sampling (p=0.0113). At coverboard stations, relative abundance declined significantly from preharvest to postharvest at a distance of 5-10 m (p=0.0163) and 40-50 m (p=0.0193) away from adjacent mature forest. Finally, using Pianka's index, I compared the night plot and coverboard sampling techniques in terms of proportions of the 4 most common species captured. These sampling techniques on average were >80% similar for all reference areas. / Master of Science

Salamanders are critical components of forest ecosystems, in terms of total biomass, as well as for their value as indicators of ecosystem stress. Considering the worldwide decline in amphibian populations, the known effects of timber harvest on salamander populations, and the importance of the forest products industry in Kentucky and elsewhere, the impacts of silvicultural operations on salamander communities cannot be overlooked. The objective was to investigate the effects of three different silvicultural treatments, each involving different streamside management zone (SMZ) characteristics, on salamander communities in ephemeral streams. Data were collected by regular checks of pitfall traps, coverboards, and transect searches. Using both pre- and post-harvest data, abundance estimates were acquired using binomial mixture models. Declines in some species of terrestrial and stream-breeding salamanders were detected, and were shown to be likely related to characteristics of the corresponding silvicultural treatment. Applying modest SMZ regulations to ephemeral streams would likely alleviate these declines significantly.

For this study, the abundance of the salamander fauna in two streams was compared to determine the impact of logging activities. After five months of study, the area around one stream was logged by the National Forest Service. Following logging, the abundance of salamanders in the stream was greatly reduced, while the population in the undisturbed stream remained relatively unchanged. Six abundant species were used as indicators; two of these species (Desmognathus quadramaculatus and Desmognathus ochrophaeus) were negatively affected by the logging, while four species (Demognathus fuscus, Desmognathus monticola, Eurycea wilderae, and Gyrinophilus porphyriticus) were not affected. Body sizes of salamanders, used to represent age class, were analyzed to determine if the age/size structures of the populations were altered. This parameter was not impacted significantly by logging; all age/size classes of the populations were equally affected.

Amphibians, because of their semi-permeable skin, sensitivity to changing microclimates, and important role in ecosystems, are often viewed as indicators of ecosystem health. They make excellent organisms for studies on the effects of silvicultural practices. My goal was to provide recommendations for forest management in the southern Appalachians so that harvesting operations are compatible with maintaining healthy populations of forest amphibians. I tested previously created habitat models that determined the most important habitat characteristics for salamanders. I counted salamanders in 240 10 x 10 m plots located in the MeadWestvaco Wildlife and Ecosystem Research Forest in north-central West Virginia. We also collected a variety of habitat data in these plots to predict salamander abundance with previously created models. These simple linear regression analyses of predicted versus observed values suggest for most models (7 out of 9) a weak relationship between predicted and observed values (R2 from 0.0033 to 0.2869, p from < 0.0001 to 0.7490). However, one of the models showed characteristics suggesting that it predicted new data as well or better than the original data, and therefore was the most accurate at predicting salamander abundance, and could be used for management purposes, although there was still much unexplained variation. This model included the variables woody stems (< 7.5 cm DBH), available rock, riparian status (i.e., within 15 m of a stream), percent overstory canopy cover, and available highly decomposed woody debris (decomposition classes 3 to 5). All of these relationships were positive except for woody stems, suggesting that in order to maintain healthy populations of salamanders, we should protect areas next to streams, with high amounts of rock, decomposed woody debris, overstory canopy cover, and few woody stems. I also examined the immediate effects of clearcuts on salamander movement and population estimates. I batch marked salamanders in plots at the edges of a clearcut, and in a control plot. Using the Schnabel estimator, I estimated population sizes in each plot. I then compared population estimates pre- and post-harvest on the interior (harvested) and exterior (unharvested) sides of the plots, taking into account the control plot. I also examined adult-juvenile ratios and movements from one side of the plot to the other. I found no significant changes (p > 0.05) following harvest in any of these measures, suggesting that salamanders do not move out of the harvested area post-harvest, at least over the short term (10 months of this study). This suggests that a longer period of time (> 1 year) is required to observe the population declines detected in most studies. / Master of Science

Due to the paucity of long-term harvest impact data, the primary goals of this study were to quantify the long-term effects of different harvest disturbances twenty-four years after harvest on two major wetland functions: stand productivity and C storage. This study evaluated the effects of three harvest types that were originally applied in 1986 to a tupelo (Nyssa aquatic)-cypress (Taxodium distichum) forested wetland in the Mobile-Tensaw River Delta of southwestern Alabama. Treatments were: 1. Helicopter harvest (HELI), 2. Skidder simulation where 50% of the site was rutted to a depth of 30 cm (SKID), and 3. Helicopter harvest followed by glyphosate herbicide removal of all sprouts and seedbank regeneration for two years following harvest (GLYPH). An adjacent mature stand (94 years old) within the same original composition represented mature forest or pre-harvest reference conditions (REF). Above- and belowground plant biomass, belowground woody debris, soil C, and soil CO2 efflux were measured. Twenty-four years after treatments were applied, forest C levels were higher in SKID treatments (206.1 Mg C ha-1) than in HELI treatments (168.7 Mg C ha-1). GLYPH treatments are holding less (144.2 Mg C ha-1) while REF areas hold 332.6 Mg C ha-1. SKID treatments are also holding the most biomass of all treatments with 243.2 Mg ha-1 of overstory biomass. Ecosystem C and biomass patterns indicate HELI and SKID are becoming similar to the original site conditions represented by the REF areas. The resiliency of these highly disturbed stands are explained by the frequent inputs of non-compacted sediments, presence of species well adapted to very poorly drained and aerated conditions, high rates of coppice regeneration, shrink-swell ameliorative properties of the soil and creation of more complex microtopography within SKID treatments. / Master of Science

We examined the short-term (1 - 4 years postharvest) effects of 7 silvicultural treatments on terrestrial salamander populations at 4 sites in southwest Virginia and West Virginia. The 3 silvicultural treatments with the most canopy removal (4-7 m2 basal area Shelterwood, Leavetree, Clearcut) had significantly fewer salamanders than the control (p < 0.10) postharvest. No differences were found among treatments in age class distribution, the percent of females that were gravid, or average clutch size. We tested the nighttime, surface-count census method for visibility and behavior-induced bias among silviculture treatments and estimated the proportion of a salamander population that is active on the surface in harvested and control habitats. Instantaneous rates of salamander activity ranged from 1.3 to 11.7% of the population for redback (Plethodon cinereus) and slimy salamanders (P. glutinosus). Timber harvest caused up to a 2-fold increase or decrease in activity rates. There was evidence for bias in the night census method, but differences were not consistent enough to suggest general bias corrections. We also tested whether poorly fed salamanders exhibited risk-sensitive foraging in a dry environment in a laboratory experiment. Poorly fed salamanders were observed out of their simulated burrows less than well fed salamanders suggesting salamanders, particularly females and small adults, are risk-averse. / Master of Science

Research documenting the functional changes of wetlands in response to harvest disturbance has often been limited to initial-response time frames of one or two years. A cypress (Taxodium distichum L. Rich.)-water tupelo ~ aQuatica L.) swamp located in the Mobile-T ensaw River Delta of Southwestern Alabama was harvested in the fall of 1986. Harvest treatments included: c1earcutting the entire study area with helicopter log removal, or with an added simulated rubber-tired skidder transport, or with herbicide control of all regenerating vegetation. An adjacent nondisturbed stand served as a reference area. Remeasurement of the area was initiated seven years after harvest. The helicopter logged and skidder simulation treatments have produced stands of comparable diameter, height, density, and basal area. ~ aqyatica was dominant in the skidder simulation treatment~ ~ tica, ~ nigra, and Fraxinus caroliniana dominated the helicopter treatment. Overstory biomass was less in the helicopter logged areas (20,981 kg/ha) than in the skidder simulation treatment (30,533 kglha). Lowerstory trees in both treatments were of comparable diameter and height, however, the helicopter treatment contained a greater density, basal area, and biomass ofFraxinus caroliniana. The rutting and mounding associated with the skidder simulation treatment favored a greater diversity of both diversity of both lowerstory and herbaceous species. Groundflora biomass was greatest in the herbicide controlled treatment (13,127 kg/ha), comparable in both the helicopter logged and skidder simulation treatments (5128 kg/ha; 5648 kglha, respectively), and least in the reference area (1495 kglha). Sediment accumulation reflected these differences in groundflora biomass; 104 mm in the herbicide controlled treatment, 79 mm and 63 mm in the helicopter logged and skidder simulation treatments, respectively, and 37 mm in the reference area. Three gradients were identified in the study areas via the tests of Latin squares, rows, and columns which actually corresponded to gradients of hydrology and! or sediment deposition. Square two was wetter than squares one or three because square two had fewer residual pullboat runs, which facilitate greater water movement. The rows are indicative of the distance from the main river channel; rows nearer the river are slightly higher in elevation, less inundated, and favor the less flood tolerant species. The columns reflect the sediment trapping patterns of the vegetation and the subsequent effects of site hydrology and nutritional status. / Master of Science

Solitary Vireos (Vireo solitarius alticola) were studied on an extensive mature forest landscape in 1994 and on three sites harvested using the group selection method in 1995. Pairing success was 88% on the unharvested site in 1994, however it was lower on the harvested sites in 1995 (53.8%). Low reproductive success was observed in both years (12% and 22% respectively). In 1995, the amount of opening within a 200 meter radius of the first observation point of a male was positively related to pairing success (p<0.05). This suggests that females may be cueing in on the increased number of shrubs in and around openings. / Master of Science

Interest in wood-based bio-energy systems in the United States is increasing and may play a part in future renewable energy initiatives (Dincer 2000). Family forests have potential to play an important role in supplying wood biomass for energy production. However, access depends mostly on the management intentions among family forest owners. Enhanced biomass markets in regions where family forest ownership dominates could increase productivity by reinvigorating the low-value merchandizing required to accomplish silvicultural objectives. Given diverse owner objectives and forest types on family forests, estimates of biomass availability must include both biophysical and social aspects of procurable feedstock. This thesis chronicles a biosocial case study that estimates potential biomass supply from 51 family forests in Virginia and North Carolina. The study occurred within a woodshed centered on the future site of an impending ethanol plant in Mecklenburg County, Virginia. A survey instrument using the theory of planned behavior was used to measure ownership characteristics and intention to harvest. Forest attributes were collected during property visits to estimate potential yields resulting from silvicultural simulations. Results reveal that forest cover-type and tree size significantly affect owner intentions to harvest and owner attitudes toward harvesting partially mediate this relationship. Outputs from silvicultural simulations correspond with those made using Forest Inventory and Analysis data within the study region. Disproportionality was examined by coupling social and biological drivers of sustainable wood biomass availability. Implications of the research include refined estimates of potential supply and demonstrating a multi-scalar, mixed-method approach for assessing wood biomass availability. / Master of Science