Cyclic and extreme ecological disturbances have the capacity to alter resources and thereby animal populations. Interactions between disturbance and resource availability can influence predator-prey interactions. Predator-prey responses to ecological disturbance may be more pronounced in herbivores and their predators as herbivores track food resources that are often augmented by ecological disturbance. My objective with this dissertation was to examine how various forms of ecological disturbance influence predator-prey interactions through the lens of a case study – white-tailed deer (Odocoileus virginianus) and Florida panther (Puma concolor coryi) in southwestern Florida public and conservation lands. I quantified species-specific behavior of deer to an extreme disturbance event (i.e., Hurricane Irma), examined behavior of females with different fate outcomes to varied ecological disturbances and predation, investigated how ecological disturbance mediates the influence of human disturbance on predator-prey interactions, and quantified deer spatial ecology in response to fire, hydrology, panther and human activity. I found that deer behaviorally mediated the negative fitness impacts of Hurricane Irma. Further, I found that female deer with different fate outcomes selected areas of different ecological disturbance and the ecological disturbance type conferred different fitness costs (through differences in predation risk). Finally, I found that South Florida deer utilize diurnal times when humans are the most active to temporally reduce predation risk as panthers were more nocturnal in response to humans. My work here suggests that ecological disturbance regimes have the capacity to influence predator-prey interactions through nuanced mechanisms. Outcomes of these nuanced species-specific and predator-prey responses should be examined further. More practically, if disturbance influences aspects of animal fitness, a deeper understanding of species-specific and predator-prey responses to disturbance will improve management and conservation efforts as some regimes can be manipulated (e.g., prescribed fire). More broadly, consideration of ecological disturbance when examining predator-prey interactions may yield novel insight that deviates from predictions based on inference suggested in systems without disturbance. Highlighting nuanced predator-prey interactions mediated by ecological disturbances will improve predictions regarding species and community responses to global changes such as climate change and ecological restoration. / Doctor of Philosophy / Events that cause temporary changes to ecosystem structure and function (ecological disturbances) have the capacity to influence resources (i.e., food and shelter) for wild animals. Changes in resources as a function of ecological disturbance has the capacity to influence prey and predator species interactions. Predator-prey responses to ecological disturbance may be more pronounced in plant-eating animals (herbivores) and their predators as herbivores utilize food resources that are often altered by ecological disturbance. My objective with this dissertation was to examine how various forms of ecological disturbance influence interactions between predators and prey by using the white-tailed deer (Odocoileus virginianus) and Florida panther (Puma concolor coryi) in southwestern Florida wildlands as a case study. I quantified species-specific behavior of deer to an extreme climate event (i.e., Hurricane Irma). Next, I quantified and compared behavioral differences in responses to ecological disturbance (flooding and fire) and panther predation risk between female deer that survived and those killed by panthers during the offspring rearing season. I also investigated how ecological disturbance and human use of wildlands influenced predator-prey interactions. Finally, I characterized deer behavior in response to fire, hydrology, and panther and human activity. I found that deer changed their behavior during Hurricane Irma, presumably to offset the negative impacts of the storm as all our monitored deer survived the event. Further, I found that different ecological conditions generated by fire and flooding, respectively, influenced female behavior during the offspring rearing season in response to predation risk, and those behavioral differences may explain differences in mortality outcomes. Finally, I found that South Florida deer utilize daylight hours when humans are the most active to minimize encounters with predators as panthers were shown to be more active at night in areas with greater human use. My findings suggest that ecological disturbances have the capacity to influence predator-prey interactions in novel ways not suggested elsewhere. Outcomes of novel predator and prey interactions in response to ecological disturbance should be investigated further. More practically, if disturbance influences aspects of animal livelihood, a deeper understanding of species-specific and predator-prey responses to disturbance will improve management and conservation efforts as some disturbances can be manipulated (e.g., prescribed fire). More broadly, consideration of ecological disturbance when examining predator-prey interactions may yield novel insight that deviates from predictions based on inference suggested in systems without disturbance. Highlighting novel predator-prey interactions that is changed as a result of ecological disturbances will improve predictions regarding species and community responses to global changes through climate change and ecological restoration.