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The Global ETD Search service is a free service for researchers
to find electronic theses and dissertations. This service is
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Networked Digital Library of Theses and Dissertations.
Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
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Functional Genetic Analysis Reveals Intricate Roles of Conserved X-box Elements in Yeast Transcriptional RegulationVoll, Sarah 13 November 2013 (has links)
Understanding the functional impact of physical interactions between proteins and
DNA on gene expression is important for developing approaches to correct disease-associated gene dysregulation. I conducted a systematic, functional genetic analysis of protein-DNA interactions in the promoter region of the yeast ribonucleotide reductase
subunit gene RNR3. I measured the transcriptional impact of systematically
perturbing the major transcriptional regulator, Crt1, and three X-box sites on the
DNA known to physically bind Crt1. This analysis revealed interactions between
two of the three X-boxes in the presence of Crt1, and unexpectedly, a significant
functional role of the X-boxes in the absence of Crt1. Further analysis revealed Crt1-
independent regulators of RNR3 that were impacted by X-box perturbation. Taken
together, these results support the notion that higher-order X-box-mediated interactions
are important for RNR3 transcription, and that the X-boxes have unexpected roles in the regulation of RNR3 transcription that extend beyond their interaction with Crt1.
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Functional Genetic Analysis Reveals Intricate Roles of Conserved X-box Elements in Yeast Transcriptional RegulationVoll, Sarah January 2013 (has links)
Understanding the functional impact of physical interactions between proteins and
DNA on gene expression is important for developing approaches to correct disease-associated gene dysregulation. I conducted a systematic, functional genetic analysis of protein-DNA interactions in the promoter region of the yeast ribonucleotide reductase
subunit gene RNR3. I measured the transcriptional impact of systematically
perturbing the major transcriptional regulator, Crt1, and three X-box sites on the
DNA known to physically bind Crt1. This analysis revealed interactions between
two of the three X-boxes in the presence of Crt1, and unexpectedly, a significant
functional role of the X-boxes in the absence of Crt1. Further analysis revealed Crt1-
independent regulators of RNR3 that were impacted by X-box perturbation. Taken
together, these results support the notion that higher-order X-box-mediated interactions
are important for RNR3 transcription, and that the X-boxes have unexpected roles in the regulation of RNR3 transcription that extend beyond their interaction with Crt1.
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