The lipopolysaccharide (LPS) translocation process was studied by a pulse-chase experimental procedure designed to follow the fate of newly synthesized LPS through the cell wall fractions of the marine bacterium Alteromonas haloplanktis, strain 214, variant 3 (ATCC 19855). It was determined that newly synthesized LPS I (the prominent LPS species under the conditions employed) initially enters an LPS fraction which can be released from the cell wall along with periplasmic material. This is followed by rapid entry of the newly synthesized LPS into a loosely bound LPS fraction (released from the cell wall by washing with NaCl) before final insertion into the outer membrane. The inhibition of protein synthesis with chloramphenicol was observed to have little or no effect on the rate of translocation of newly synthesized LPS. In contrast, both the respiratory inhibitor NaCN (10 mM) and the proton ionophore 3,3',4',5-tetrachlorosalicylanilide (TCS, 30 (mu)M), if added during the chase period, resulted in an immediate and complete inhibition of further LPS translocation. The results suggest an independence of the LPS transloction process from continued protein synthesis, but a requirement for an energy source. It was observed that newly synthesized LPS sedimented to a lower density position than previously formed LPS on sucrose density gradients, and displayed a faster migration rate during electrophoresis on sodium dodecyl sulfate polyarylamide gels (SDS-PAGE). This indicated compositional differences between the two LPS populations. Extended electrophoresis on SDS-PAGE separated both newly synthesized and previously formed LPS I into a number of distinct radiolabelled peaks and shoulders, indicating compositional microheterogeneity within each species. The fate of added radiolabelled galactose was also investigated. A one minute pulse with {('14)C} galactose resulted in a preferential labelling of components associated with the cell wall such that 57.3% of the total w

Tillage erosion is a major contributor to the total soil erosion in cultivated topographically complex lands. No study has been carried out on tillage erosion associated with cereal-based production systems in the Canadian Prairies, and there is a need to examine tillage erosivity of secondary tillage and seeding implements and the effect of slope curvature on tillage translocation. With both tillage and water erosion occurring in a cultivated topographically complex landscape, it is valuable to investigate the relative contributions of and the possible linkage and interactions between these two erosion processes. Tillage translocation causes the mixture of subsoil into the till-layer, which may considerably affect soil properties and therefore the related biophysical processes. In this study, using plot tracers, we examined tillage translocation caused by four tillage implements: air-seeder, spring-tooth-harrow, light-cultivator and deep-tiller in southern Manitoba, Canada. We determined that secondary tillage and seeding implements could be as erosive as primary tillage implements in a cereal-based production system. In the majority of cases, tillage translocation could be explained by slope gradient alone; however, slope curvature also significantly affected tillage translocation. In two field sites in the North America Great Plains (NAGP), measured 137Cs inventories were converted into total soil erosion rates. Tillage and water erosion rates were estimated using models. The comparisons of the model estimates to 137Cs estimates showed that both tillage and water erosion significantly contributed to the total soil erosion in undulating slopes while tillage erosion was the predominant erosion process in hummocky hilltops. The contributions of and the linkage and interactions between water and tillage erosion showed predictable patterns in different landform elements, with the knowledge of which, landscape segmentation could be used to assess the potential of soil erosion. Further investigation of tillage translocation was demonstrated with four hypothetic landscapes: plane slope, symmetric hill, asymmetric hill and irregular hill, and is tested against field data. A Visual Basic coded program (TillTM) was developed to simulate the redistribution of soil constituents and soil mass. We determined that the pattern of soil mass redistribution was dependent on topography, while the pattern of soil constituent redistribution was affect by topographic features, tillage patterns and temporal scales.

Tillage erosion is a major contributor to the total soil erosion in cultivated topographically complex lands. No study has been carried out on tillage erosion associated with cereal-based production systems in the Canadian Prairies, and there is a need to examine tillage erosivity of secondary tillage and seeding implements and the effect of slope curvature on tillage translocation. With both tillage and water erosion occurring in a cultivated topographically complex landscape, it is valuable to investigate the relative contributions of and the possible linkage and interactions between these two erosion processes. Tillage translocation causes the mixture of subsoil into the till-layer, which may considerably affect soil properties and therefore the related biophysical processes. In this study, using plot tracers, we examined tillage translocation caused by four tillage implements: air-seeder, spring-tooth-harrow, light-cultivator and deep-tiller in southern Manitoba, Canada. We determined that secondary tillage and seeding implements could be as erosive as primary tillage implements in a cereal-based production system. In the majority of cases, tillage translocation could be explained by slope gradient alone; however, slope curvature also significantly affected tillage translocation. In two field sites in the North America Great Plains (NAGP), measured 137Cs inventories were converted into total soil erosion rates. Tillage and water erosion rates were estimated using models. The comparisons of the model estimates to 137Cs estimates showed that both tillage and water erosion significantly contributed to the total soil erosion in undulating slopes while tillage erosion was the predominant erosion process in hummocky hilltops. The contributions of and the linkage and interactions between water and tillage erosion showed predictable patterns in different landform elements, with the knowledge of which, landscape segmentation could be used to assess the potential of soil erosion. Further investigation of tillage translocation was demonstrated with four hypothetic landscapes: plane slope, symmetric hill, asymmetric hill and irregular hill, and is tested against field data. A Visual Basic coded program (TillTM) was developed to simulate the redistribution of soil constituents and soil mass. We determined that the pattern of soil mass redistribution was dependent on topography, while the pattern of soil constituent redistribution was affect by topographic features, tillage patterns and temporal scales.

Diss. (sammanfattning) Umeå : Univ., 2004. / Härtill 5 uppsatser.

Thesis (Ph. D.)--University of Texas at Austin, 2002. / Vita. Includes bibliographical references. Available also from UMI Company.

Thesis (Ph. D.)--University of Texas at Austin, 2006. / Vita. Includes bibliographical references.

Visualization of the transport pathway in barley (Hordeum vulgare) leaves was carried out using a combination of aniline blue and a symplasmically transported fluorochrome, 5,6 carboxyfluorescein (5,6-CF). When applied to a source leaf, basipetal movement of 5,6-CF was observed after 3 h and the fluorochrome front was observed about 3-4cm away from the point of application. The fluorochrome was taken up into the symplasm of the mesophyll and was loaded into the bundle sheath cells and then subsequently the vascular parenchyma and finally into the sieve tubes. In sink leaves, acropetal movement was observed after 3 h and the fluorochrome had moved approximately 3 cm away from the point of application. Unloading of 5,6-CF occurred from all classes of longitudinal veins. Studies on solute retrieval showed that 5,6 CF-diacetate was transferred to xylem parenchyma where it was metabolized. 5,6-CF was then transferred from the xylem parenchyma to the vascular parenchyma cells, and it would appear that thick-walled sieve tubes were the first to show 5,6-CF labeling. Counterstaining with aniline blue demonstrates the presence of plasmodesmata and this suggests a potential symplasmic pathway from the mesophyll to the sieve tubes. Application of 5,6 CF-diacetate revealed a slow symplasmic pathway, which involved transfer of 5,6-CF, which was effected via plasmodesma.

No description available.

No description available.

No description available.