Global ETD Search

1	Paracrine factors of vascular endothelial cells facilitate cardiomyocyte differentiation of mouse embryonic stem cells 日高, 京子, Hidaka, Kyoko, 三輪, 佳子, Miwa, Keiko, 室原, 豊明, Murohara, Toyoaki, 笠井, 謙次, Kasai, Kenji, 佐賀, 信介, Saga, Shinsuke, 森崎, 隆幸, Morisaki, Takayuki, 上田, 裕一, Ueda, Yuichi, 児玉, 逸雄, Kodama, Itsuo January 2008 (has links) No description available. Embryonic stem cell Cardiac cell Vascular endothelial cell Paracrine factor Cardiomyogenesis Nkx2.5 Bone morphogenic protein Wnt Cyclooxygenase Nitric oxide synthase
2	CD31(-) HipOps - A Highly Osteogenic Cell Population From Mouse Bone Marrow McKenzie, Kristen Penny 04 December 2012 (has links) Multipotent mesenchymal stem cells (MSCs), found in many adult tissues, may be useful for regenerative medicine applications. Their identification and purification have been difficult due to their low frequency and lack of unambiguous markers. Using a magnetic micro-beads negative selection technique to remove contaminating hematopoietic cells from mouse bone marrow stromal cells (BMSCs), our lab recently isolated a highly purified osteoprogenitor (HipOp) population that was also enriched for other mesenchymal precursors, including MSCs (Itoh and Aubin, 2009). To further enhance enrichment, we positively selected BMSCs and HipOps for CD73, a putative MSC marker, which resulted in no significant additional enrichment for osteoprogenitors when the population was tested in vitro. However, we also found that HipOps were enriched in vascular endothelial cells, and that removing these cells by further negative selection with CD31/PECAM resulted in a CD31(-) HipOp population with higher osteogenic capacity than HipOps in vitro and in vivo. mesenchymal stem cell osteoprogenitor osteoblast MACS magnetic bead cell sorting cell sorting osteogenesis CD31 PECAM cell culture negative selection cell marker bone marrow stromal cell BMSC HipOp bone marrow vascular endothelial cell limiting dilution analysis accessory population CD73 0379
3	CD31(-) HipOps - A Highly Osteogenic Cell Population From Mouse Bone Marrow McKenzie, Kristen Penny 04 December 2012 (has links) Multipotent mesenchymal stem cells (MSCs), found in many adult tissues, may be useful for regenerative medicine applications. Their identification and purification have been difficult due to their low frequency and lack of unambiguous markers. Using a magnetic micro-beads negative selection technique to remove contaminating hematopoietic cells from mouse bone marrow stromal cells (BMSCs), our lab recently isolated a highly purified osteoprogenitor (HipOp) population that was also enriched for other mesenchymal precursors, including MSCs (Itoh and Aubin, 2009). To further enhance enrichment, we positively selected BMSCs and HipOps for CD73, a putative MSC marker, which resulted in no significant additional enrichment for osteoprogenitors when the population was tested in vitro. However, we also found that HipOps were enriched in vascular endothelial cells, and that removing these cells by further negative selection with CD31/PECAM resulted in a CD31(-) HipOp population with higher osteogenic capacity than HipOps in vitro and in vivo. mesenchymal stem cell osteoprogenitor osteoblast MACS magnetic bead cell sorting cell sorting osteogenesis CD31 PECAM cell culture negative selection cell marker bone marrow stromal cell BMSC HipOp bone marrow vascular endothelial cell limiting dilution analysis accessory population CD73 0379

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