Thesis (Ph. D.)--University of California, San Diego and San Diego State University, 2005. / Vita. Includes bibliographical references.

Thesis (M.S.)--University of Wisconsin--Madison, 1970. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (M.S.)--Oregon State University, 1994. / Typescript (photocopy). Includes bibliographical references (leaves 48-56). Also available on the World Wide Web.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1953. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 127-142).

Bibliography: leaves 135-156. This study investigated the effects of the symbiotic association of plants with vesicular-arbuscular mycorrhizal fungi on the intensity of intraspecific competition and its consequences on popular structure. Four main glasshouse experiments were performed using the non-cultivated Rhodanthe chlorocephala ssp. Rosea, and the cultivated species Trifolium subterraneum grown at different plant densities, under different levels of phosphorus and light, and in environments with homogeneous and patchy distribution of phosphorus. Results emphasise that the main effects of mycorrhizas at the individual level may not by apparent at population level because of the influence of density-dependent processes. However, infected individuals with a strong response to the symbiosis would have an advantage in situations of competition.

No description available.

<p>The long-range aim of this thesis was to further the understanding of the mechanisms involved in virus interference. To this end, biochemical and immunological procedures were used to characterize and define the virus-specific proteins of VSV. Biological studies of homotypic and heterotypic ·interference were also carried out. </p> / Thesis / Doctor of Philosophy (PhD)

The potential of human adenovirus (Ad) to serve as a vector for expression of heterologous genes was evaluated. An experimental gene, consisting of sequences coding for the glycoprotein of vesicular stomatitis virus (VSV) attached to the promoter and polyadenylation signal of the herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) gene, was inserted into early region 3 of adenovirus, in both orientations. The TK promoter was functional in both orientations. The TK promoter was functional in both orientations and responded to trans-activation by HSV infection. Abundant expression of VSV G however depended on the presence of a second transcript. This transcript was present only in the recombinant carrying the insertion in the orientation parallel to the E3 promoter (AdG12) and was initiated upstream of the insertion, within Ad sequences. The potential of Ad recombinants to serve as vaccine vectors was investigated using the recombinant AdG12. Antibody against VSV G was induced in cows, pigs, and dogs in response to infection with AdG12. Protection of mice, immunized with AdG12, against a lethal challenge with VSV demonstrated the biological effectiveness of this immune response. / Thesis / Master of Science (MS)

No description available.

Diante da necessidade de responder algumas indagações relacionadas a epidemiologia da Estomatite Vesicular, principalmente aquelas que dizem respeito a ocorrência de surtos em locais onde existem coleções d\'água, foi desenvolvido um modelo de transmissão do VSA utilizando a água como via de transmissão, a tilápia nilótica, inoculada intraperitonealmente, como fonte de infecção e o cobaio como hospedeiro susceptível. O objetivo da utilização deste modelo biológico de transmissão do Vírus da Estomatite Vesicular foi de avaliar o papel desempenhado pelos peixes no ciclo epidemiológico, propor um modelo de ciclo epidemiológico do VSA, destacando o papel da água como via de transmissão e padronizar uma técnica de RT-PCR para a detecção do VSA, em amostra de tecidos. Através do modelo desenvolvido, fica demonstrado que estes peixes eliminaram partículas virais na água, decorridos 13 dias pós-inoculação e que esta última se caracteriza como via de transmissão, possibilitando a infecção dos hospedeiros susceptíveis (cobaios) através de inoculações experimentais em coxim plantar. A tilápia nilótica pode ser considerada como uma fonte de infecção, por ser capaz de eliminar um agente infeccioso no meio ambiente e através de uma via de transmissão este agente alcançou o hospedeiro susceptível; os peixes podem ser inseridos no ciclo epidemiológico da Estomatite Vesicular como fonte de infecção, sendo capazes de eliminar na água partículas virais infectantes, destacando o papel da água como via de transmissão; fica padronizada uma técnica de RT-PCR dirigida ao gene codificador da proteína RNA-polimerase, útil para a detecção direta do Vírus da Estomatite Vesicular Alagoas e Indiana em amostras de tecidos. / A model of transmission of Vesicular Stomatitis was developed to Vesicular Stomatitis Alagoas (VSA) serotype employing water as a way of transmission, the Nile tilapia intraperitoneal inoculated as a source of infection and guinea pigs as susceptible hosts aiming to answer many questions concerning Vesicular Estomatitis epidemiology, as the risk of disease on farms with dose relationship with riverine areas and the role of fishes in the epidemiological cycle of the disease. Furthermore, a RT-PCR assay was developed to detect VSA in tissue samples. According to the experimental transmission, fishes eliminated virus into the water after 13 days pos-infection and a model to VSA epidemiological cycle is proposed in which water was characterized as a way of transmission, carrying the virus to the susceptible host through experimental inoculation and the Nile tilapia should be thought as a source of infection, once it was able to eliminate the infective agent into the environment. A useful tool to the diagnosis of both Indiana and Alagoas serotypes was developed.