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Drought stress was studied in rice (Oryza sativa) and maize (Zea mays) to identify drought-responsive genes and associated biological processes. One experiment with rice examined drought responses in vegetative and reproductive tissues and identified drought-responsive genes in each tissue type. The results showed that brief periods of acute drought stress at or near anthesis reduced photosynthetic efficiency and ultimately lowered grain yield. Yield was reduced as a result both of fewer spikelets developed and of lower spikelet fertility. Affymetrix arrays were used to analyze global gene expression in the transcriptomes of rice vegetative and reproductive tissue. Comparative analysis of the expressed genes indicated that the vegetative and reproductive tissues responded differently to drought stress. An experiment was conducted with maize, using GS-FLX pyrosequencing to identify differentially expressed genes in vegetative and reproductive tissues; and these results were compared with those from the just-described rice transcriptome. Some of the drought-responsive genes in the maize reproductive tissue were validated by quantitative real time polymerase chain reaction (qRT-PCR). The differentially expressed genes common to both maize and rice were further analyzed by gene ontology analysis to reveal core biological processes involved in drought responses. In both species, drought caused a transition from protein synthesis to degradation, and photosynthesis was one of the most severely affected metabolic pathways. In a validating experiment, a drought-responsive transcription factor found in rice and dubbed HIGHER YIELD RICE (HYR) was constitutively expressed in rice, and the transgenic HYR plants were studied. Under well-watered conditions, the HYR plants developed higher rates of photosynthesis, greater levels of soluble sugars (glucose, fructose, and sucrose), more biomass, and higher yield. They also exhibited a drought-resistant phenotype, with higher water use efficiency, photosynthesis, and relative leaf water content under drought stress. Taken together, these studies demonstrate the potential value of newer technologies for identifying genes that might impart drought resistance and for using such genes to make crops more productive either in the presence or in the absence of drought stress. / Ph. D.

The need for a more sustainable approach to water consumption has increasingly gained attention in the last decade. The domestic sector accounts for over half of abstracted water in the UK and, as such, has become a major target for water efficiency interventions. Current research and water efficiency interventions are dominated by a positivist approach, focusing on a limited range of factors that can be quantitatively measured. This thesis questions the dominant approach and argues that a more holistic overview of water efficiency can be achieved through the consideration of socio-technical and behavioural theories. Taking a more constructivist approach, this research draws on four theories from socio-technical and behavioural fields and combines them to create a framework for the analysis of water efficiency interventions. The framework is applied to two case studies, exploring water users¿ perceptions of water, water supply, personal water use, and their responses to the water efficiency interventions. The case studies were selected to provide examples of current mainstream approaches to water demand management. Research methods used included semi-structured interviews and observation. The research findings support the argument that the current dominant approach to domestic water efficiency interventions is limited and, in some cases, ineffectual. Issues of trust, knowledge, motivation and the relationships between water users and water companies were raised. The thesis concludes that the use of a constructivist perspective could help to provide a more effective approach to understanding and improving water demand management.

Forest plantations have been long-employed to reverse land degradation and support biodiversity, and are now recognized to both take in atmospheric carbon dioxide, reducing the intensity of the greenhouse effect, and moderate local weather. It is important to consider the impact forest aging and management will have on provisioning of these services under climate change and extreme weather events, such as drought. This study encompasses a chronosequence of three Eastern White Pine stands planted in 1939, 1974 and 2002, situated in Turkey Point, Ontario, Canada. The oldest forest received two selective thinning treatments, removing 30% of trees, in 1983 and 2012. Forest water use efficiency (WUE), which represents the amount of gross ecosystem productivity (GEP) per unit of water released through evapotranspiration (E), was compared among the three sites over 2008-2013. The youngest forest’s annual WUE increased over the study period, surpassing that of the older sites by 2013. When bulk surface conductance (Gs), representing gas exchange, was compared across the sites for the same years, the youngest site had the lowest Gs, particularly during drought. Gs at the oldest forest was highest and the most variable. Statistical analysis showed that across all the sites, E was more responsive to air temperature than atmospheric demand, soil moisture, and incident radiation. This study indicated that younger plantations may be more water-conservative during drought, and that air temperature is important to consider in projections of temperate coniferous forests’ carbon and water exchange. To assess the impact of the 2012 selective thinning on tree-level and ecosystem-level water use at the oldest forest, sapflow velocity (Js), transpiration (Et) and E were compared between the two stands planted in 1939 and 1974, from 2011 to 2013. A relatively severe drought over the 2012 growing season led to a decline in Et at the unthinned site for that year, however the Et decline was more pronounced at the older, thinned site. From 2011 to 2012, Js increased at the thinned site, converse to the unthinned site – wherein Js was low as expected during drought. Hydraulic redistribution and lag time from sapflow at 1.3 m height to canopy evapotranspiration were seemingly unaffected by the thinning, indicating that low-level selective harvesting was not detrimental to the hydrological functionality of the stand, and may have been beneficial in allowing more soil moisture access per tree. As such, the stand may be better positioned to withstand recurrent dry spells resulting from precipitation variability, as predicted with climate change. / Thesis / Master of Science (MSc)

Short rotation woody crops (SRWC) are bred for rapid growth properties. Knowledge of how varying environmental conditions and endophytic bacteria impact physiology are needed to make planting recommendations. Three eastern cottonwood (EC) and three hybrid poplar (HP) varietals were planted in replicate blocks at upland and alluvial sites. Whole-tree water use and water use efficiency (WUE) were measured using heat-dissipation sap flow and related to overall productivity. Productivity measurements were higher at the upland site. Sap flow was higher at the upland site and for endophyte treated individuals. WUE was higher at the alluvial site and for EC. WUE and leaf area index (LAI) were significantly correlated with biomass (negatively and positively respectively). Overall, HP performed better at the upland site, EC at the alluvial site. These results are beneficial in further testing of optimal site and genotype pairings for SRWC.

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One goal of this research was to characterize shoot tissue-level responses in loblolly pine to soil moisture limitation in combination with fertilization as well as to more severe soil moisture limitation. We found that neither fertilization alone, nor fertilization in combination with soil moisture limitation resulted in changes to shoot tissue water relations parameters classically characterized in drought response studies. More severe water limitation was necessary to elicit responses, and those responses had not been fully described previously. The more severe water limitation resulted in increased capacitance beyond turgor loss, increased relative water content at turgor loss, a more negative turgor loss point, an increased bulk modulus of elasticity, more negative osmotic potential at 100% relative water content, and an increased apoplastic water fraction. As there were indications of reduced water use and moisture stress in the absence of shoot level responses under less severe drought, such parameters are insufficient alone to characterize moisture stress in fertilized and in less severely water limited loblolly trees. Additionally, we sought a morphological or physiological explanation for the reduced transpiration and increased water use efficiency reported for fertilized trees in the Virginia Piedmont. Our characterizations of the responses of root distribution and hydraulics to limited soil moisture here complement existing research, which demonstrated changes to root distribution and hydraulics in response to fertilization. The responses we discovered in fertilized trees that accompanied reduced transpiration and increased water use efficiency that differed from responses to reduced soil moisture alone were primarily large decreases to shallow root presence. We found this to be readily quantified using measures of root length density. Decreases to whole-tree hydraulic conductivity were also shown to occur with fertilization and were shown not to occur in shoot tissue, suggesting limitation via rhizosphere or root xylem conductance. Our results support the supposition that fertilization narrows hydraulic safety margins and potentially predisposes loblolly trees to moisture stress, particularly prolonged, severe water limitation following fertilization. Finally, we tested the validity of throughfall exclusion for simulating reduced rainfall using a greenhouse 'split-pot' study, which applied spatially fixed heterogeneous soil moisture to young, well-watered loblolly pines. The 'split-pot' experiments demonstrated that spatially fixed soil moisture heterogeneity does not confound drought effects; needle area specific transpiration was not decreased, nor was water use efficiency increased. This supports the validity of inferences taken from drought simulation experiments with loblolly pine where throughfall exclusion troughs reduce soil moisture content in a consistent, spatially heterogeneous manner. / Doctor of Philosophy / We investigated various effects of soil moisture limitation alone, and in combination with common fertilization practices in loblolly pine production. Responses at the shoot and needle level to different levels of soil moisture limitation produced new findings concerning how tissues respond to more severe water limitation. A 30% decrease in throughfall precipitation alone, or in combination with fertilization did not elicit drought related shoot tissue responses despite the presence of other indications of moisture stress and reduced water use. We also sought to explain why fertilized trees experiencing water limitation had environmental sensitivities that were different from unfertilized tree receiving ambient rainfall amounts or from trees only experiencing water limitation without fertilization. We found that changes to shallow root presence, especially root length density, accompanied the different patterns of environmental sensitivity and water use. Also, the water conducting ability of roots changed unevenly in soil with uneven moisture levels. The ability of roots to resist loss of conductivity to water did not change unevenly in the same way. We did another set of experiments to determine if using impervious troughs to catch rain is a valid approach to reducing soil moisture for the purpose of testing how loblolly responds to water limitation. These throughfall exclusion troughs create uneven soil moisture reduction, which can have effects on plant water use that are separate from water limitation alone. We found that in well-watered young trees, uneven soil moisture alone did not produce responses that could be confused with the effects of water limitation. This finding indirectly validates the use of throughfall exclusion troughs to simulate reduced rainfall.

Increasing drought and salinity tolerances in economically important trees adapted to dry land areas is key challenge for maintaining the socioeconomic welfare of dry land areas. Strategies to improve drought and salt tolerance must examine the tree physiological mechanisms that link to the trees survival and growth. This study examined physiological adaptive traits allowing Senegalia senegal to grow better in both saline and dry lands. We conducted two greenhouse experiments and one field study to characterize growth, photosynthetic capacity, water use efficiency (WUE) and gum arabic yield potential among different Senegalia senegal provenances. In the first experiment, we tested early growth and photosynthetic response of seed sources to a cyclic drought treatment. The second greenhouse study examined seed source response to drought, salinity and fertility conditions. Gum yield assessment, growth and WUE of mature trees were determined from a field trial. In general, results showed a high intra genetic variability of Senegalia senegal on gum yield, biomass accumulation and growth. Ngane provenance presented superior growth characteristics as both mature trees and seedlings and exhibited a more conservative water use strategy under drought. Both greenhouse studies revealed similar photosynthetic capacity among Senegalia senegal genotypes when conditions are not limiting. However, when factors such as salinity, fertility and severe drought are involved, different physiological and morphological responses appear and at times this was dependent on seed source. But, at moderate drought stress (chapter 2), no drought by provenance interaction was found. Results of chapter 3 revealed that Ngane has larger stomata with low density in comparison with Diamenar and Kidira provenances. With the exception of Ng21B1, all seed sources displayed similar adaptations to salt stress in term of biomass accumulation. Fertilizer increased total biomass of all seed sources from 63% to 213% for Ng21B1 and K17B19, respectively. However, salinity reduced the fertilizer effect on biomass increment. Leaf gas exchanges were affected by salinity and fertilizer within various responses among seed sources. Results of chapter 4 revealed that gum yield was found to be positively correlated with tree height, crown width, stem volume index and crown area index. Ngane and Diamenar appeared the best provenances in term of annual gum yield per hectare. Diamenar had a higher survival rate than Ngane. This finding reveals the need to consider the tradeoff between tree survival rate and individual tree gum yield in Senegalia senegal stands. In addition to being more sensitive to salinity, Ngane also seems to be more susceptible to low soil pH in terms of survival, but this result needs to be tested further. This study suggests that improvement of gum arabic production can be possible through genetic selection. But, for the best adapted genotypes, research should explore new genetic combination and investigate physiology and genetic diversity. Moreover, the findings on the relationship between soil pH and tree survival rate suggests a need for care in selecting appropriate sites for Senegalia senegal stands. Therefore, silviculture practices as well as genetic selection appear critical in improving gum arabic production. / Ph. D.