Deformable mirror is a widely used wavefront corrector in adaptive optics system, especially in astronomical, image and laser optics. A new structure of DM-3D DM is proposed, which has removable actuators and can correct different aberrations with different actuator arrangements. A 3D DM consists of several reflection mirrors. Every mirror has a single actuator and is independent of each other. Two kinds of actuator arrangement algorithm are compared: random disturbance algorithm (RDA) and global arrangement algorithm (GAA). Correction effects of these two algorithms and comparison are analyzed through numerical simulation. The simulation results show that 3D DM with removable actuators can obviously improve the correction effects.

A new type of adaptive optics (AO) based on the principles of digital holography (DH) is proposed and developed for the use in wide-field and confocal retinal imaging. Digital holographic adaptive optics (DHAO) dispenses with the wavefront sensor and wavefront corrector of the conventional AO system. DH is an emergent imaging technology that gives direct numerical access to the phase of the optical field, thus allowing precise control and manipulation of the optical field. Incorporation of DH in an ophthalmic imaging system can lead to versatile imaging capabilities at substantially reduced complexity and cost of the instrument. A typical conventional AO system includes several critical hardware pieces: spatial light modulator, lenslet array, and a second CCD camera in addition to the camera for imaging. The proposed DHAO system replaces these hardware components with numerical processing for wavefront measurement and compensation of aberration through the principles of DH. We first design an image plane DHAO system which is basically simulating the process the conventional AO system and replacing the hardware pieces and complicated control procedures by DH and related numerical processing. In this original DHAO system, CCD is put at the image plane of the pupil plane of the eye lens. The image of the aberration is obtained by a digital hologram or guide star hologram. The full optical field is captured by a second digital hologram. Because CCD is not at the conjugate plane of the sample, a numerical propagation is necessary to find the image of the sample after the numerical aberration compensation at the CCD plane. The theory, simulations and experiments using an eye model have clearly demonstrated the effectiveness of the DHAO. This original DHAO system is described in Chapter 2. Different from the conventional AO system, DHAO is a coherent imaging modality which gives more access to the optical field and allows more freedom in the optical system design. In fact, CCD does not have to be put at the image plane of the CCD. This idea was first explored by testing a Fourier transform DHAO system (FTDHAO). In the FTDHAO, the CCD can directly record the amplitude point spread function (PSF) of the system, making it easier to determine the correct guide star hologram. CCD is also at the image plane of the target. The signal becomes stronger than the image plane DHAO system, especially for the phase aberration sensing. Also, the numerical propagation is not necessary. In the FTDHAO imaging system, the phase aberration at the eye pupil can be retrieved by an inverse Fourier transform (FT) of the guide star hologram and the complex amplitude of the full field optical field at the eye pupil can be obtained by an inverse FT of the full field hologram. The correction takes place at the eye pupil, instead of the CCD plane. Taking FT of the corrected field at the eye pupil, the corrected image can be obtained. The theory, simulations, and experiments on FTDHAO are detailed in chapter 3. The successful demonstration of FTDHAO encourages us to test the feasibility of putting CCD at an arbitrary diffraction plane in the DHAO system. Through theoretical formulation by use of paraxial optical theory, we developed a correction method by correlation for the general optical system to perform the DHAO. In this method, a global quadratic phase term has to be removed before the correction operation. In the formulation, it is quite surprising to find that the defocus term can be eliminated in the correlation operation. The detailed formulations, related simulations, and experimental demonstrations are presented in Chapter 4. To apply the DHAO to the confocal retinal imaging system, we first transformed the conventional line-scanning confocal imaging system into a digital form. That means each line scan is turned into a digital hologram. The complex amplitude of the optical field from each slice of the sample and aberration of the optical system can be retrieved by digital holographic process. In Chapter 5, we report our experiments on this digital line-scanning confocal imaging system. This digital line-scanning confocal image absorbs the merits of the conventional line-scanning confocal imaging system and DH. High-contrast intensity images with low coherent noise, and the optical sectioning capability are made available due to the confocality. Phase profiles of the samples become accessible thanks to DH. The quantitative phase map is even better than that from the wide field DH. We then explore the possibility of applying DHAO to this newly developed digital line-scanning confocal imaging system. Since optical field of each line scan can be achieved by the DH, the aberration contained in this field can be eliminated if we are able to obtain the phase aberration. We have demonstrated that the phase aberration can be obtained by a guide star hologram in the wide field DHAO systems. We then apply this technique to acquire the aberration at the eye pupil, remove this aberration from the optical fields of the line scans and recover the confocal image. To circumvent the effect of phase aberration on the line illumination, a small collimated laser beam is shone on the cylindrical lens. Thus the image is solely blurred by the second passage through the aberrator. This way, we can clearly demonstrate the effect of DHAO on the digital line-scanning confocal image system. Simulations and experiments are presented in chapter 6, which clearly demonstrates the validity of this idea. Since line-scanning confocal imaging system using spatially coherent light sources has proven an effective imaging tool for retinal imaging, the presented digital adaptive optics line-scanning confocal imaging system is quite promising to become a compact digital adaptive optics laser scanning confocal ophthalmoscope.

Las aberraciones ópticas determinan la formación de imágenes en el ojo, tanto en el proceso de la visión como en las observaciones oftalmoscópicas del fondo de ojo. La corrección total de estas aberraciones permitiría una resolución limitada sólo por la difracción en las pupilas utilizadas. Las aberraciones del ojo difieren de un sujeto a otro y no responden a modelos sencillos. En este trabajo se propone el uso de técnicas de Óptica Adaptativa para el desarrollo de un sistema experimental para la medida y corrección de las aberraciones estáticas del ojo. Estas técnicas pueden ser igualmente útiles para obtener imágenes de alta resolución de la retina, utilizarse en el diseño de lentes oftálmicas, etc. Para la medida de la función aberración de onda, se han utilizado dos métodos no invasivos aplicables al ojo humano: La Recuperación de Fase a partir de dos imágenes de Doble Paso, y el Sensor de Hartmann-Shack. Para la corrección de la aberración se ha utilizado un Modulador Espacial de Cristal Líquido.Se han desarrollado los procedimientos de control y de calibrado de estos métodos, y se estudia la viabilidad de aplicación para el ojo. Finalmente, se han realizado medidas de la aberración, mediante ambos métodos, y su posterior corrección mediante el modulador espacial de cristal líquido, en un ojo artificial y en sujetos reales. / The image formation properties of the eye are determined by the aberrations of the optics. The complete correction of the aberrations would allow diffraction-limited resolution. The aberrations of the eye are not easily modeled and are different for each subject.This thesis proposes the use of adaptive optics techniques to measure and correct the static aberrations of the eye. The principles and methods developed are useful in specific applications, i.e., high-resolution retinal imaging, ophthalmic lens design, etc.Two non-invasive methods have been used to measure the wave aberration function: Phase Retrieval Techniques from two double-pass retinal images; and the Hartmann-Shack sensor. A Liquid Crystal Spatial Light Modulator was used to adaptively correct the wave front aberration of the eye.This thesis also includes guidelines to calibrate and control the proposed techniques.Finally, experimental explorations of these methods are reported. Several results are presented, including the measure and the subsequent compensation of the wave aberration for artificial and human eyes.