The problems associated with short growing seasons has led to the development of leafy-reduced stature (LRS) corn hybrids. These hybrids have more leaf area above the ear, more rapid leaf area development, shorter stature, earlier maturity, and better responses to high plant populations and narrow spacings than conventional hybrids. Plants grown in a reduced light environment are limited in carbon assimilation and this, in turn, results in reductions in growth and development. A way to supplement the availability of photosynthate is injection of sucrose into plant stems. The objective of this thesis was to determine the ability of LRS corn plants to compete with weeds, and the reactions of weed species to the shade, including the relationships between weed growth (increase in biomass) and development (shape) under shaded conditions. Three years of field experiments (LRS and more conventional corn hybrids with both transplanted and naturally growing weeds) and two years of greenhouse work [weeds alone, C3 (lamb's quarters and velvetleaf) and C4 (redroot pigweed) in full sun or deep (75%) shade injected with 15% sucrose or not] were conducted. Yield reductions due to weed pressure were lower for LRS than other hybrids. Biomass production by both transplanted and naturally occurring weeds was up to 85% less under corn canopies than when grown without competition from corn. The biomass of C4 weeds was more reduced by competition with corn plants than that of C3 weeds. In spite of quick and early leaf development, leaves and other plant parts of LRS were not damaged excessively by mechanical (rotary hoeing) weed control. Both C3 and C4 weed plants produced more dry matter when injected with sucrose. Dry weights of sucrose injected shaded plants were not different from full sun uninjected plants. However, sucrose injection did not alter shading effects on development (distribution of biomass). Dry matter production and photosynthetic rates of C4 weeds were more reduced

Dwarf apple tree growth and yield under several non-herbicidal ground management systems were compared. Six ground cover systems were established in two newly planted orchards. After two growing seasons, trees in the straw mulch and geotextile mulch treatments had larger trunk cross-sectional areas and more shoot growth than trees in the red fescue and insectary plant cover treatments. Growth of trees under the manure mulch and cultivation treatments was intermediate. When four of the systems were established along rows of five-year old trees in an existing orchard, the increase in trunk cross-sectional area over two growing seasons was greatest for trees in the manure mulch and straw mulch followed by tose in geotextile mulch, trees in the red fescue grew least. The use of a mulch, such as straw or geotextile, has a favourable effect on growth and has potential for use in organic orchards.

Control of Taraxacum officinale (dandelion) and other broadleaf weeds in turfgrass has been readily achieved with phenoxy herbicides, but the herbicide option has been revoked in many regions, necessitating alternative weed control strategies. One biological alternative is Sclerotinia minor, an Ascomycete fungus. The impact of S. minor on broadleaf weed dynamics and biotic interactions were studied in a turfgrass environment. The goal was to maximize effectiveness of a S. minor formulation as a biocontrol of dandelion using an ecological approach. S. minor efficacy was not affected by turf microenvironments and was similarly efficacious with spring or fall application. All accessions from a worldwide collection of dandelion and 32 turfgrass broadleaf species were susceptible to S. minor. Biocontrol efficacy was inversely correlated with dandelion age, but efficacy on all ages was enhanced in the presence of grass competition. When combined with regular mowing at 7-10 cm, the S. minor suppressive effect on dandelion was similar to the herbicide effect, particularly in the following season. Weed suppression was less with close mowing at 3-5 cm due to increased dandelion seedling recruitment. While spring herbicide application was effective to suppress dandelion population, the S. minor treatment has no residual activity, necessitating a second application to suppress seedling recruits. Root regrowth after S. minor infection was minimal and was further reduced in superior quality turf after season-long mowing, and after spring applications. S. minor infected dandelion seeds, reduced the dandelion seedbank, and reduced dandelion seedling emergence by 98%. S. minor did not affect the emergence or the total biomass of cool season temperate turfgrass species. Turfgrass quality was improved following S. minor application and populations of other broadleaf weeds were also controlled by S. minor. Understanding the biotic interactions within the turfgrass environment has rewardingly lead to successful integration of the S.minor biocontrol with the common management tools of mowing and over-seeding to achieve excellent control of dandelion and a healthy thriving turf.

Field trials were conducted to evaluate the effect of quizalofop-ethyl on quackgrass plants in a potato cropping sequence. Fall and summer applications were compared for their quackgrass control potential. Season-long quackgrass control was obtained with quizalofop-ethyl at 96 g/ha following summer application. An increase in the rate of quizalofop-ethyl did not further improve control. Yields with quizalofop-ethyl at 96 g/ha were similar to standard treatments sethoxydim and fluazifop-butyl at recommended rates. Quackgrass control following a summer application was not maintained through to the following season. Fall applications did not result in adequate control of quackgrass the following season at any of the quizalofop-ethyl rates tested.

Lettuce plants (Lactuca sativa L. var. Ithaca) were transplanted on different beige and black paper mulches and on a coextruded white/black polyethylene mulch in organic soil in 1997 and 1998. Non-weeded and weeded control plots were also used. Plant mortality in 1997 for plants grown on bare soil was 10 to 15% greater than that of mulched plants. Head weight was found to be 3.6 times (1997) and 12 times (1998) greater for lettuce grown on mulch compared with a weeded control. The transmission, reflection and absorption of light of the white/black polyethylene mulch and black paper mulch remained stable over the course of the experiment while those of the other papers changed over time. Paper mulch decomposition occurred during the experiment. Although, black paper mulch was the only mulch that gave complete control of weed growth, weed populations present under the beige papers were too small in terms of the size and number of weeds to cause lettuce yield reduction.

Coleomegilla maculata is a beneficial coccinellid commonly found in sweet cornfields in Kentucky. Previous work on C. maculata has shown an ovipositional preferencefor the weed Acalypha ostryaefolia, compared to three selected weed species and corn. Also, predation of C. maculata egg clusters on A. ostryaefolia was less compared toclusters on corn and the presence of A. ostryaefolia led to higher densities of C. maculata larvae on corn. I determined C. maculata ovipositional preference among weed species in fieldtests using nine common weeds. I also examined ovipositional preference using just A.ostryaefolia and Abutilon theophrasti. I assessed the roles of potential prey densities,plant structures, and weed attractiveness to adult C. maculata. Finally, I examineddiurnal and nocturnal predation of C. maculata eggs on corn, A. ostryaefolia, A.theophrasti, and Amaranthus hybridus. Significant ovipositional preference was always observed for A. theophrasti. C. maculata egg clusters on A. theophrasti and A.ostryaefolia were preyed upon less frequently than clusters on A. hybridus and corn.

Subtilisin-like proteases form a large group of serine proteases with diverse functions, including the specific processing of a variety of proproteins and prohormones, and are found in prokaryotic and eukaryotic organisms. The work in this thesis focuses mainly on the Aral2 subtilisin-like protease following its discovery in the filtrate of Arabidopsis cell suspension cultures. Evidence obtained by Southern blotting and database searching is presented for the existence of a large gene family encoding subtilisin-like proteases in the model plant Arabidopsis thaliana. There may be more than fifty members in this gene family. Three of the corresponding DNA sequences have been cloned by RT-PCR and used as probes in Northern analysis to investigate the tissue specificity of the gene transcripts. These three genes appear to be expressed to varying degrees in Arabidopsis leaf, stem, root and silique tissues. A 650bp cDNA fragment encoding the C-terminal portion of the Aral2 protease has been obtained by RT-PCR, ligated to the malE gene and overexpressed as a fusion protein in E. coli cells. Polyclonal antisera have been raised against a combination of the fusion protein and the Aral2 C-terminal protein purified after cleavage from the fusion protein using Factor Xa protease. Aral2 protein has been detected in Arabidopsis tissues, particularly in siliques and stems, by Western blotting using these antibodies. An apoplastic location has been ascribed to Aral2 protease by immunocytochemistry using electron microscopy. The mature Aral2 subtilisin-like protease has been purified to homogeneity from Arabidopsis cell suspension culture filtrate by ion exchange chromatography and hydrophobic interaction chromatography. The purified enzyme has an acidic pH optimum of approximately pH5.5, which is unusual for a plant subtilisin-like protease. Aral2 protease is relatively thermostable and is activated in the presence of Ca(^2+) ions. The known serine protease inhibitors phenylmethanesulphonyl fluoride (PMSF), 4-(2- aminoethyl) benzenesulphonyl fluoride (AEBSF) and diisopropyl fluorophosphates (DFP) have an inhibitory effect on the proteolytic activity of Aral2. Substrate specificity studies have been performed using artificial peptide substrates, native proteins and cell wall protein extracts from Arabidopsis cells.

This thesis reports the design and control of the ??GreenWeeder??, a non-herbicidal autonomous weeding robot, in order to autonomously track crop rows for weeding through electrocution in the inter-row space. The four wheel mobile robot platform was designed and built with a motorised Ackerman steering system allowing the robot to steer up to 30 degree left and right. It was also equipped with an electronically geared rear wheel drive, a pair of stereo cameras, a SICK LMS-291 laser range finder to localize itself with respect to the crop rows, a GPS system for obtaining the robot position in the field and a long-range communication system for tele-supervision by operators. The first prototype of the robot electrocution system was also designed and constructed to ignite 22kV electrical arcs to destroy weeds. Its operation was tested in the research field of the University of Sydney and the results of this experiment were analysed to improve the efficiency of this first prototype. An improved prototype of the electrocution system was then constructed and attached to a cradle extending out at the back of the mobile robot platform. The testing of this improved prototype was conducted at Lansdowne farm, a research field of the University of Sydney. After the construction of the robot platform, the robot control was considered with the demands for robot localization with respect to crop rows, an autonomously tracking control system and a manual control mode in order to take the robot to transportation vehicles. Firstly, the robot localization was accomplished by utilizing SICK LMS-291 laser range finder sensor for the sensing and perception of the robot. Secondly, the robot control system was developed with a PID controller, a second order model of the robot system and a first order filter. The PID controller is in the standard form with the filtered derivative and the PI part being in automatic reset configuration. The second order model was identified using Matlab System Identification toolbox based on the robot kinematic analysis. The first order filter is utilized for filtering out the lateral deviations of the robot with respect to the crop rows received from the SICK laser sensor. A Simulink simulation model of the robot control system was also built in order to fine-tune PID and filter parameters. Thirdly, the manual control mode of the robot was produced. In this mode, a joystick can be attached to a notebook to wirelessly drive the robot around or it can be plugged into a USB port at the back of the robot to drive it without the notebook. After the robot control was implemented and simulated, some experiments were conducted with the robot autonomously tracking a strip of reflective tape mimicking a crop row stuck into the ground of a laboratory. Depending on distances from the row assigned to the controller, the robot tried to keep those distances away from the row. The results showed the lateral errors of the robot with respect to the row were approximately 4.5 cm which were sufficient for our current agricultural application.

Bibliography: leaves 209-254. / xv, 254 leaves : ill. (chiefly col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Experiments detailed in this thesis, evaluate the impact of various herbicides and herbicide mixtures on the growth, nodulation and nitrogen fixation of annual pasture legumes, the efficacy of the herbicides for weed control, and the potential role of mechanical defoliation to replace herbicides, leading to lower cost and more sustainable farming systems. / Thesis (Ph.D.)--University of Adelaide, Dept. of Plant Science, 1996

Orobanche ramosa L. is an annual, parasitic weed present in the western Murray-Mallee region of South Australia. A quarantine zone was established to encompass all known infestations, and has been adjusted over time as new infestations have been discovered. The movement of fodder, machinery, grain and straw, horticultural crops, livestock, and soil is controlled by strict quarantine procedures, to prevent further spread across the landscape. O. ramosa presents a unique situation for weed managers: plants are obligate parasites, relying entirely on broadleaved hosts for their water and nutrition; and seeds are tiny (0.3 mm), produced in large numbers (up to 100 000 seeds per plant), and are long-lived, persisting in the soil seed bank for up to 13 years. The dispersal vectors for O. ramosa in South Australia are the focus of this Master’s thesis. Two dispersal vectors were chosen for investigation: sheep and wind. Sheep were examined as possible vector for seeds, both via the gut (internal transport, or endozoochory) and via adhesion on the external surface of the animal (external transport, or epizoochory). Internal transport via sheep was investigated with a classic gut-passage experiment, which showed a peak in excretion of weed seeds at day 2, reducing to zero seeds excreted at day 8, and a half-life of 2 days. Two phases of external transport on sheep was studied: attachment and retention. Attachment was confirmed by finding seeds on the body wool and feet of sheep that had been kept for 7 days on soil with an O. ramosa seedbank. Seed retention was confirmed by placing seeds onto the body and still finding them in wool samples after 7 days. Wind was the other dispersal vector investigated for O. ramosa. A survey of natural wind dispersal was conducted, which confirmed wind as a vector and allowed trap design to be tested. Then a portable field-based wind tunnel was used to investigate the effects of ground cover (bare ground and cereal stubble) and wind speed (low, medium and high) on wind dispersal of O. ramosa seeds. For the stubble treatments, more seeds were trapped within the tunnel, and on bare ground more seeds were trapped exiting the tunnel. Importantly, the data showed that low wind speeds readily move O. ramosa seeds, and that the seeds are capable of aerodynamic lift in the wind profile. Results are discussed in the context of dispersal biology, quarantine procedures, and future work that would further refine knowledge of likely dispersal vectors for O. ramosa. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1459246 / Thesis (M.Sc.) -- University of Adelaide, School of Earth and Environmental Sciences, 2009