The formation of an objectionable "swampy" odour in drinking water distribution systems in Perth, Western Australia, was first described by Wajon and co-authors in the mid-1980s (Wajon et al., 1985; Wajon et al., 1986; Wajon et al, 1988). These authors established that the odour, variously described as "swampy", "sewage" or "cooked vegetable" was caused by dimethyltrisulfide (DMTS) which has an odour threshold concentration of 10 nanograms per litre (ng/L). Investigations described in the present Thesis extend the work of Wajon and co-workers in attempting to establish the origin and cause of DMTS formation in Perth drinking water distribution systems.The DMTS problem appeared to be confined to water originating from a particular type of groundwater, specifically groundwater sourced from shallow, unconfined aquifers, which contain relatively high concentrations of sulfide, dissolved natural organic matter (NOM) and dissolved iron. DMTS was not present in the groundwater, but only formed in the distribution system, after treatment of groundwater via alum coagulation-filtration and oxidation processes. One objective of the present work was to determine the reasons for the observed association between DMTS formation and this specific groundwater type. A primary focus was to investigate the chemistry and biochemistry of sulfur species and NOM which might act as precursors to DMTS. The work was driven by the view that increased understanding of the problem might lead to more effective and acceptable treatment solutions than those presently in use.The observation that DMTS forms in distributed water that originates from groundwater, but not in water from surface sources has led to the hypothesis that groundwater NOM may contain precursor(s) to DMTS For example, it was proposed that methyl esters and ethers within humic substances might be a source of methyl groups that ++ / could participate in DMTS formation in distributed water (Wajon and Heitz, 1995; Wajon and Wilmot, 1992). Further, comparison of levels of reduced sulfur with levels of dissolved organic carbon (DOC) in groundwaters feeding Wanneroo GWTP revealed that a positive correlation between these two parameters existed. This observation provided further impetus to examine the nature of NOM in these groundwater systems. In the present study (discussed in Chapter 3), NOM from two Perth drinking water sources was isolated and characterised, with the aim of identifying major differences in structure and/or functional groups that might influence DMTS formation. NOM was isolated from water samples using ultrafiltration, and characterised using pyrolysis gas chromatography-mass spectrometry (Py-GC-MS) and offline- thermochemolysis/methylation (TCM). Pyrolysis of groundwater NOM yielded a high proportion of organosulfur compounds, primarily methyl thiophenes and sulfur gases, but did not yield detectable amounts of methoxy-aromatic compounds. Analysis by TCM yielded sulfur compounds tentatively identified as the methyl esters of methylthiopropanoate and methylthiobutanoate, compounds that may arise as degradation products of dimethylsulfoniopropionate (DMSP), an algal odmoregulator Compounds such as DMPS could potentially undergo reactions to form DMTS in distributed water.The task of investigating the formation of nanogram-per-litre concentrations of DMTS demanded the development of new analytical procedures that could be used to determine similarly low concentrations of DMTS precursors. Evidence existed to suggest that inorganic polysulfides could be plausible precursor compounds, and since no technique existed to analyse and quantify individual polysulfide homologues a new technique needed to be developed and verified. The technique, first used in a semiquantitative manner by ++ / Wajon and Heitz (1995), utilizes methyl iodide to derivatise polysulfides in-situ. The technique was developed further and shown to be quantitative and specific for inorganic polysulfides. Further, a new procedure for the determination of d i methyl polysulfides (DMPSs; CH3SnCH3, where n = 2-5), based on purge and trap was developed. In this new procedure analytes were trapped on a "Grob" activated charcoal tube, which was integrated into a commercially available, automated purge and trap instrument. Perdeuterated analogues of the DMPS analytes were synthesized and used as internal standards. These modifications resulted in a more rapid and robust procedure than the previously used procedures, vii which were based on closed loop stripping analysis (CLSA). Validation of the precision, accuracy, linearity and robustness of the new procedures for both inorganic polysulfides and dimethylpolysulfides is described in Chapter 4.Previous authors (Wajon and Heitz, 1995; Wajon and Wilmot, 1992; Wilmot and Wajon, 1997) hypothesized that DMTS could arise in the distribution system from residual polysulfides or other reduced sulfur compounds originating from groundwater. The latter authors showed that a small proportion of sulfide in the groundwater was not completely oxidised to sulfate during the water treatment process and proposed that this residual reduced sulfur fraction, which they referred to as non-sulfide reduced sulfur (NSRS) could contain precursors to DMTS. In a review of the chemistry of sulfide oxidation (Chapter 2) it was shown that the most likely forms of sulfur comprising the NSRS that enters the Wanneroo distribution system are organosulfur compounds and elemental sulfur, probably associated with organic matter in the form of a sulfur sol.Analysis of inorganic polysulfides in treated water, using the newly described method in Chapter 4, revealed that small ++ / amounts of these compounds (20-80 ng/L) were occasionally present in some samples. However, it was concluded that, since inorganic polysulfides could not survive water treatment processes, these compounds probably arose from traces of biofilm or pipe sediment that may have entered the water during sampling. It was proposed that the presence of biofilm particulates in water samples probably also accounted for observations that DMTS appeared to form in some water samples during storage of the sample. These studies are discussed in Chapter 5.The primary method of control of DMTS formation in the distribution system has been to maintain free chlorine residuals. However, the mechanisms by which this occurs have not been studied; the effectiveness of DMTS oxidation by chlorine, or how chlorine affects microbial processes that might form DMTS is not known. These issues are addressed in the final section of Chapter 5. Experiments to determine the effectiveness of oxidation of dimethyldisulfide (DMDS) and DIVITS (5 mu g/L) by free chlorine (0.2 to 0.6 mg/L) in distributed water showed that these substances are rapidly and completely oxidised in water containing a chlorine residual of more than 0.4 mg/L. However, slow regeneration of traces of DMDS and DIVITS after dissipation of free chlorine to non-detectable levels showed that these compounds were incompletely oxidised at the lower chlorine concentrations~ This provides some rationale for field observations that DIVITS occurs even where low, but measurable, chlorine residuals appear to exist (<0.2 mg/L).As was established in a review of the chemistry of reduced sulfur compounds Chapter 2), reducing conditions not present in the oxic bulk water are required for DMTS to form and to persist. It was therefore proposed that microbial reduction processes could generate anoxic microniches in the distribution system, within which ++ / DMTS production could occur. This hypothesis was investigated in Chapter 6; the new methods for analysis of organic and inorganic polysulfides were applied to the study of biofilms and deposits of colloidal material found in distribution pipes and storage reservoirs. The study demonstrated that these materials contained concentrations of methylated and inorganic polysulfides four to six orders of magnitude higher than those ever found in the bulk water phase. The results indicated that reducing conditions most probably exist within the biofilms and pipewall deposits, where these polysulfides were formed. The iron-rich pipe slimes appeared to protect the sulfur compounds against the oxidative effects of chlorine and dissolved oxygen. It was concluded that the organic and inorganic polysulfides most probably arise through microbial sulfate reduction processes that occur in anoxic microenvironments within the slimes and deposits.Microbial processes that lead to the formation of polysulfides and dimethylpolysuifides under conditions approximately representative of those in distribution systems were investigated in work described in Chapter 7. The aim of this work was to investigate the role of biofilms in the formation of DMTS and to determine the nature of chemical precursors which might stimulate these processes. Biofilms, artificially generated on synthetic supports within chambers filled with water from Wanneroo GWTP, were exposed to compounds thought to be potential DMTS precursors. The response of the systems in terms of production of methylated sulfur compounds was monitored. Conclusions of the study were that, under the test conditions, production of DMDS and DMTS could occur via several mechanisms and that these dimethyloligosulfides could be formed even without the addition of compounds containing sulfur or methyl moieties. DMTS did not form in the absence of ++ / biofilms and it was therefore concluded that minimisation of biofilm activity was a key in preventing DMTS formation. Outcomes of the work imply that environments within distribution systems are complex and dynamic, as perhaps manifested by the intermittent nature of the DMTS problem.Finally, in Chapter 8 the conclusions to the present studies are summarised. It is shown how they underpin the rationale for proposed new treatment solutions aimed at preventing DMTS problems in the Wanneroo zone, primarily by minimising microbial activity and biofilm formation within distribution systems.

Thesis (Ph.D.)--Murdoch University, 2002. / Thesis submitted to the Division of Arts. Bibliography: leaves 388-412.

Thesis (Ph.D.)--University of Western Australia, 2006.

Thesis (Ph.D.)--University of Western Australia, 2007.

The distribution pattern of diatoms from lakes and streams of varying salinity in the south-west of Western Australia was investigated. A total of 95 water bodies were sampled and separated into freshwater (<3 ppt), hyposaline (3-20 ppt), mesosaline (20-50 ppt) and hypersaline (>50 ppt). The south-west and specifically the inland wheatbelt region has been severely influenced by secondary salinisation, due to clearing of native vegetation for agriculture. There has been little research on diatom communities from salt-affected systems, with this data providing the basis for the development of an inference model based on species optima and tolerance limits to salinity. Physico-chemical variables measured from the study sites were collated and assessed. Salinity ranged from freshwater (0.04) to hypersaline (156.80 ppt), and pH ranged from acidic (2.90) to alkaline (10.51). Dissolved oxygen levels were recorded from 1.11 to 18.67 mgL-1, water temperature from 6.30 to 28.10 °C and peripheral vegetation scores from 1 (little or no cover) to 5 (dense cover). Analysis of variance (ANOVA) showed that salinities were significantly higher in standing waters located further inland, compared to flowing waters in high rainfall areas. Hypersaline wetlands displayed significantly lower dissolved oxygen levels, higher water temperatures and reduced peripheral vegetation, compared to freshwaters. The pH of hypersaline sites was also significantly lower, associated with surrounding land use or underlying geology. The data collected provides important baseline information, with implications for aquatic biota. / The community structure of diatoms in relation to varying salinity concentration was explored. An artificial substrate collector (JJ periphytometer) was used to standardise sampling and ensure diatom assemblages were representative of ambient water quality. A total of 217 taxa were identified with the highest diversity observed in freshwater sites (up to 33 species), and limited to less than 15 in hypersaline waters. According to BIOENV, salinity was the key factor influencing diatom community structure. SIMPER analysis found a number of discriminating taxa between salinity ranges, specifically between assemblages from freshwater and hypersaline sites. Taxa such as Achnanthidium minutissimum and Gomphonema parvulum were indicative of freshwaters. In comparison, Amphora coffeaeformis and Nitzschia ovalis were associated with hypersaline water bodies. Diatom community structure was also examined from 20 hypersaline wetlands in the wheatbelt region with varying pH. Characteristic taxa including Amphora coffeaeformis, Hantzschia sp. aff. baltica and Nitzschia ovalis showed a wide tolerance to salinity and pH, or hypersaline acidic conditions. BIOENV analysis found there were no observable differences between diatom assemblages in relation to salinities above 50 ppt and that pH was highly correlated to species composition. The increasing occurrence of acid saline lakes is of concern and is most likely attributable to deep drainage practices and continued use of fertilisers in agricultural areas. / A diatom-based transfer function was developed from the south-west dataset, to document species optima and tolerance limits to salinity. CCA analysis showed that salinity accounted for a significant and independent amount of variation in the diatom data enabling an inference model to be derived. The most successful model was generated using tolerance-downweighted weighted averaging, with a high coefficient of determination and low prediction errors that remained high after jackknifing. The optima of freshwater diatoms were similar to those reported from other regions of the world, although the optima of hypersaline species tended to be higher. Comparatively, the model performed very well, with the potential to be applied in future paleolimnological studies. In conclusion, this study has shown diatoms to be effective biomonitoring tools, providing the basis for future sampling strategies that assess the biodiversity of salt-affected water bodies in Western Australia. Potential indicator species from different salinity ranges were identified and the sensitivity of diatoms allowed for the development of a statistically robust inference model for the south-west. The reported optima and tolerance limits of important taxa may be further explored to evaluate the success of remediation measures implemented for secondary salinised systems in this region.

[Truncated abstract] In south-western Australia a rare plant community occurs on shallow soils overlaying massive ironstone rock. These 'ironstone communities' are open shrublands, which are subject to extremes in drought and solar radiation and support many rare and endemic species. The restricted distribution of many of these species may be related to their high degree of specialisation to this harsh habitat and their inability to respond plastically to different environmental conditions. Indeed, earlier work has shown that ironstone Hakea species (Proteaceae) have a specialist root-system morphology investing mainly in deep roots, thereby increasing their chance of accessing cracks in the rock surface and obtaining water before the onset of summer drought. In this thesis I further examine aspects of specialisation and its possible consequences for species rarity using two ironstone Hakea species and comparing them with two of their widely distributed congeners. In the first experiment (Chapter 2) I explore inherent drought tolerance, independent of root-system morphology, as a further specialisation to the ironstone environment. All species were grown in sand in pots in a glasshouse for 7 months and then droughted for 5 weeks. There was no evidence that the ironstone species had a greater inherent drought tolerance than their common congeners. During drought all species maintained leaf water content of mature leaves by reducing stomatal conductance and osmotically adjusting, though ironstone species tended to OA (osmotic adjustment) more than common species. ... This suboptimal investment of resources may result in a lower competitive ability in shadier environments, and thus could partially explain their restricted distribution. In Chapter 4, I investigated the plasticity of root traits in response to levels of phosphorus supply. South-western Australian soils are phosphorus impoverished and phosphorus is well known to elicit plastic responses in root allocation and architecture. Ironstone species showed less plasticity in total root length, producing similar root length across P treatments, while common species showed an increase in root length with increasing [P]. Other root characteristics were similarly plastic in response to P treatment between species. However, when supplied with increasing [P], ironstone species invested an increasing proportion of roots in the bottom of pots while common species invested more in the top. This differential response in root allocation in response to P may reflect a fundamental trade-off between nutrient and water acquisition, with the ironstone species mainly foraging for water and investing in deeper roots, while the common species invest more in superficial roots to obtain nutrients. In conclusion, the rarity and restricted distribution of the ironstone Hakea species may be related to their specialist root-system morphology as well as a lowered phenotypic plasticity of functional traits. A reduction in plasticity may reduce their competitive ability outside their ironstone habitats, and thus contribute to the restricted distribution of these species. This may also be the case for other rock-outcrop endemics and more generally, for other rare plant species restricted to particular habitats where a lowered phenotypic plasticity in traits relevant to their particular habitat may contribute to their restricted distribution.

[Truncated abstract] The salinisation of agricultural land, urban infrastructure and natural habitat is a serious and increasing problem in southern Australia. Government funding has been allocated to the problem to attempt to reduce substantial costs associated with degradation of agricultural and non-agricultural assets. Nevertheless, Government funding has been small relative to the size of the problem and therefore expenditure needs to be carefully targeted to interventions that will achieve the greatest net benefits. For intervention to be justified, the level of salinity resulting from private landholder decisions must exceed the level that is optimal from the point of view of society as a whole, and the costs of government intervention must be less than the benefits gained by society. This study aims to identify situations when government intervention is justified to manage dryland salinity that threatens to affect road infrastructure (a public asset). A key gap in the environmental economics literature is research that considers dryland salinity as a pollution that has off-site impacts on public assets. This research developed two hydrological/economic models to achieve this objective. The first was a simple economic model representing external costs from dryland salinity. This model was used to identify those variables that have the biggest impact on the net-benefits possible from government intervention. The second model was a combined hydro/economic model that represents the external costs from dryland salinity on road infrastructure. The hydrological component of the model applied the method of metamodelling to simplify a complex, simulation model to equations that could be easily included in the economic model. The key variables that have the biggest impact on net-benefits of dryland salinity mitigation were the value of the off-site asset and the time lag before the onset of dryland salinity in the absence of intervention. ... In the case study of dryland salinity management in the Date Creek subcatchment of Western Australia, the economics of vegetation-based and engineering strategies were investigated for road infrastructure. In general, the engineering strategies were more economically beneficial than vegetation-based strategies. In the case-study catchment, the cost of dryland salinity affecting roads was low relative to the cost to agricultural land. Nevertheless, some additional change in land management to reduce impacts on roads (beyond the changes justified by agricultural land alone) was found to be optimal in some cases. Reinforcing the results from the simple model, a key factor influencing the economics of dryland salinity management was the urgency of the problem. If costs from dryland salinity were not expected to occur until 30 years or more, the optimal response in the short-term was to do nothing. Overall, the study highlights the need for governments to undertake comprehensive and case-specific analysis before committing resources to the management of dryland salinity affecting roads. There were many scenarios in the modelling analysis where the benefits of interventions would not be sufficient to justify action.

[Truncated abstract] Most of Australia, and large areas of many other continents, is drained by intermittent rivers and streams, however comparatively few biogeochemical studies have been completed for these systems. Intermittent, dryland streams are highly dynamic environments subject to unpredictable and sporadic flow. Natural disturbance from lengthy drought periods and sudden floods are typical for these systems. Without adequate baselines for natural disturbances, it is difficult to quantify other effects from anthropogenic disturbance such as dewatering, land clearing, and urbanisation, or climate change. This thesis presents work from a four-year study examining the biogeochemistry of nitrogen (N), phosphorus (P) and carbon (C) in soils and sediments of two intermittent streams (Barnett Creek and Pirraburdoo Creek) in the Pilbara region of north-west Australia. The Pilbara is an area of ancient geology and highly weathered environments that is undergoing rapid development yet is poorly understood from an ecological perspective. The principal objectives of this thesis were to determine: i) how flooding affects the spatiotemporal patterns of nutrients in intermittent stream landscapes; ii) the role of flooding in N and C mineralisation and microbial dynamics; and iii) the connections between benthic algae, microbes and nutrient availability in channel sediments. To address these objectives, three field studies and two incubation experiments were conducted. Field studies at Barnett Creek indicated that flooding reduced the spatial heterogeneity of available soil nutrients and microbes in the stream landscape, and that topography (relative elevation) in the stream landscape was of less importance in influencing nutrient and microbial patterns than flooding or landscape position. ... Field studies at Pirraburdoo Creek indicated that microbial biomass and activity increased in benthic algal mats during mat senescent stages, and decreased after flooding when mat biomass peaked. Benthic algae grew rapidly in gravel run environments after flooding, while declining in pools, and demonstrated moderate N limitation and strong P limitation. Pools had two to eight times greater NO3-N, three to five times more total N, and two to three times more labile P, OC and total C than either pools after flooding, or runs before or after flooding. Hence, the pools at Pirraburdoo Creek represented a local, interflood store of nutrients in otherwise nutrient-poor landscape, when connectivity to upstream reaches or upland environments was weak or non-existent. This thesis provides the first detailed analysis of soil and sediment biogeochemical responses to flooding for intermittent streams in the Pilbara region and for semi-arid Australia. Further pressing questions raised by this work include: What is the key pulse size and frequency for maintaining Pilbara riparian communities as well as soil microbial function? How do the spatio-temporal nutrient and microbial patterns observed persist over (i) multi-decadal scales, (ii) mega-spatial (larger landscape to regional) scales, (iii) different flood frequency-magnitude regimes, and (iv) different stream sizes? Stream biogeochemistry is a burgeoning field, and it is therefore reasonable to expect such existing gaps in knowledge may be addressed in the near future.

[Truncated abstract] Perth is the capital city of Western Australia (WA) and the home of more than three quarters of the population in the state. It is located in the southwest WA (SWWA), a low to moderate seismic region but the seismically most active region in Australia. The 1968 ML6.9 Meckering earthquake, which was about 130 km from the Perth Metropolitan Area (PMA), caused only minor to moderate damage in PMA. With the rapid increase in population in PMA, compared to 1968, many new structures including some high-rise buildings have been constructed in PMA. Moreover, increased seismic activities and a few strong ground motions have been recorded in the SWWA. Therefore it is necessary to evaluate the seismic risk of PMA under the current conditions. This thesis presents results from a comprehensive study of seismic risk of PMA. This includes development of ground motion attenuation relations, ground motion time history simulation, site characterization and response analysis, and structural response analysis. As only a very limited number of earthquake strong ground motion records are available in SWWA, it is difficult to derive a reliable and unbiased strong ground motion attenuation model based on these data. To overcome this, in this study a combined approach is used to simulate ground motions. First, the stochastic approach is used to simulate ground motion time histories at various epicentral distances from small earthquake events. Then, the Green's function method, with the stochastically simulated time histories as input, is used to generate large event ground motion time histories. Comparing the Fourier spectra of the simulated motions with the recorded motions of a ML6.2 event in Cadoux in June 1979 and a ML5.5 event in Meckering in January 1990, provides good evidence in support of this method. This approach is then used to simulate a series of ground motion time histories from earthquakes of varying magnitudes and distances. ... The responses of three typical Perth structures, namely a masonry house, a middle-rise reinforced concrete frame structure, and a high-rise building of reinforced concrete frame with core wall on various soil sites subjected to the predicted earthquake ground motions of different return periods are calculated. Numerical results indicate that the one-storey unreinforced masonry wall (UMW) building is unlikely to be damaged when subjected to the 475-year return period earthquake ground motion. However, it will suffer slight damage during the 2475-return period earthquake ground motion at some sites. The six-storey RC frame with masonry infill wall is also safe under the 475-year return period ground motion. However, the infill masonry wall will suffer severe damage under the 2475-year return period earthquake ground motion at some sites. The 34-storey RC frame with core wall will not experience any damage to the 475-year return period ground motion. The building will, however, suffer light to moderate damage during the 2475-year return period ground motion, but it might not be life threatening.

Potato (Solanum tuberosum L.) varieties Atlantic and Granola are widely grown in Indonesia. The optimal method of cultivation in the tropics, due to the susceptibility of cut seed for disease, is by small (20 to 55 g) whole seed potatoes. However, the variety Atlantic produces mostly large tubers, which are not suitable for planting as whole seeds. Although Granola produces a reasonable proportion of small tubers it still produces a few in the larger size grades and there is no fresh market in Western Australia for the larger tubers for this variety. The aim of this study was to develop methods to be used in Western Australia that improve the yield of small seed potatoes for export to Indonesia. The influence of seed-potato storage duration (at 4°C) on subsequent stem growth was assessed after 30 days growth in a glasshouse (22°C/18°C, day⁄night). Seed potato storage for 22-28 (Atlantic) and 24-30 (Granola) weeks resulted in development of higher numbers of stems. A series of field experiment were designed to increase yield of small tubers. Apical sprout removal in Granola, but not Atlantic, increased the number of stems (by 27%), yield of 20-55 g potato (by 32%) and total yield (by 17%). Application of herbicide (paraquat + diquat) at low concentration during early tuber initiation decreased total yield in Atlantic (by 14%) and Granola (by 16%). Treating whole seed potatoes with carvone vapor two weeks before planting had no influence on stem or tuber number in both Atlantic and Granola but in Atlantic only, the total yield was reduced by 12%. Spraying plants with paclobutrazol during early tuber initiation inconsistently influenced tuber number and yield between the two varieties and two experiments. The influence of gibberellic acid (GA3) on stem number, total tuber number, yield of 20-55 g tubers and total yield was investigated by dipping seed pieces in a GA3 solution (20 mg⁄L) two days prior to planting. In Atlantic, GA3 treatment increased stem number (by 147%), total tuber number (by 75%) and yield of 20-55 g tubers (by 330%) without influencing total yield. In Granola, GA3 treatment increased stem number (by 50%), total tuber number (by 15%), yield of 20-55 g tubers (by 21%) and total yield (by 10%) The influence of gibberellic acid application (20 mg⁄L) to seed pieces before planting increased the number of small tubers through increased stem number. The shift toward a greater proportion of small tubers, without reducing total yield, had a greater influence in Atlantic than that in Granola. Treatment of GA3 and paclobutrazol together decreased total yield compared to that of GA3 alone