New technologies arise everyday, but which ones are the best for your project? How do you store data in a safe manner that complies with the new European GDPR law? How do you plan, develop and complete a project? These are but a few formulations of questions that the study in this report will cover.  The report is based around a business idea and vision for a company that offers advanced products built with modern technologies. The first step for the development of this company is to create a prototyp of its first product, a web platform. The prototyp will be formed after a projectplan with meetings with a supervisor and with the use of professional tools like Gantt, WBS and Trello. First and foremost a study will be done behind the theory of different frameworks and technologies but also a reasearch about GDPR and data security. The design phase will be the first part in shaping the projekt where wireframes, sitemap and ERdiagram have been created and documented. The choice of frameworks, mainly Vue, Express and MongoDB and the data security standards JWT and Oauth will be explained and used in the prototype as a base for the technical aspects of the project. Lastly the results och the finished prototype will be presented together with a discussion regarding problems that arose during the project, improvments that needs to be done and what the future holds for the complete web platform. / Nya tekniker växer i rasande fart, men vilka är bäst för ens projekt? Hur lagrar man data på ett säkert sätt som följer den nya europeiska GDPR lagen? Hur planerar, utvecklar och genomför man ett projekt? Dessa är några få frågeställningar som undersökningen i denna rapport tar tittar närmare på.  Rapporten grundar sig på en affärsidé och vision för ett företag som erbjuder avancerade produkter skapade med moderna webbteknologier. Det första steget för företagets utveckling är att skapa en prototyp av den första produkten, en webbplattform. Prototypen kommer att utvecklas efter en projektplan med handledningsmöten och användning av professionella verktyg som Gantt, WBS och Trello. Först och främst studeras teorin bakom om olika ramverk och tekniker men även en undersökning om GDPR och datasäkerhet. Designfasen kommer sedan vara det första momentet för projektets utformning där bland annat wireframes, sitemap och ERdiagram har skapats och dokumenterats. Valet av ramverk, främst Vue, Express och MongoDB samt datasäkerhetsstandarna JWT och Oauth kommer att förklaras och användas i prototypen som grund för projektets tekniska skapande.  Slutligen presenteras resultatet av den färdiga prototypen tillsammans med en diskussion över problem som uppstod under projektets gång, förbättringar som behöver göras och vilka framtidsplaner som finns för den fullständiga webbplattformen.

My diploma thesis is focused on creating an application for Electronic Register of Sales. The main sense of the application is to facilitate an implementation to e-shops, which were created on so-called "Greenfield". This application is supposed to help programmers in their work, because the most complex logic will be done by the app. Another big advantage of it is saving money to the e-shop owners. The implementation of my solution is more time saving than the implementation which is provided by Financial administration of the Czech Republic. The first part of my thesis is concentrated on Electronic Register of Sales issue. It's following by a description of technology and tools which I used for the app development. The selected technology and tools are the most modern and widely used nowadays. The end of the theoretical part is about introducing e-shop Tisknisi.cz In the practical part of the thesis I described the "E-EET" application and compiled its structure in terms of frontend and backend. Then I explained its technical solution. Code samples are accompanied by explanations. A phase "implementation solution" is described by POST request method. I've designed a price policy to ensure the profitability of "E-EET" application by myself.

The field of DNA nanotechnology offers a wide range of design strategies with which nanometer-sized structures with a desired shape, size and aspect ratio can be built. The most established techniques in the field rely on close-packed 'solid' DNA nanostructures produced with either the DNA origami or the single-stranded tile techniques. These structures depend on high-salt buffer solutions and require more material than comparable size hollow wireframe structures. This dissertation explores the construction of hollow wireframe DNA nanostructures composed of equilateral triangles. To achieve maximal material efficiency the design is restricted to use a single DNA double helix per triangle edge. As a proof of principle, the DNA origami technique is extended to produce a series of truss structures including the flat, tetrahedral, octahedral, or irregular dodecahedral truss designs. In contrast to close packed DNA origami designs these structures fold at low-salt buffer conditions. These structures have defined cavities that may in the future be used to precisely position functional elements such as metallic nanoparticles or enzymes. The design process of these structures is simplified by a custom design software. Next, the triangulated construction motif is extended to the single-stranded DNA tile technique. A collection of finite structures, as well as one-dimensional crystalline assemblies is explored. The ideal assembly conditions are determined experimentally and using molecular dynamics simulations. A custom design software is presented to simplify the design and handling of these structures. At last, the cost-effective prototyping of triangulated wireframe DNA origami structures is explored. This is achieved through the introduction of single-stranded “gap” regions along the triangle edges. These gap regions are then filled using a DNA polymerase rather than by synthetic oligonucleotides. This technique also allows the mechanical transformation of these structures, which is exemplified by the transition of a bent into a straight structure upon completion of the gap filling.:Abstract v Publications vii Acknowledgements ix Contents xi Chapter 1 A short introduction into DNA nanotechnology 1 1.1 Nanotechnology 1 1.1.1 Top down 1 1.1.2 Bottom up 3 1.2 Deoxyribonucleic acid (DNA) 4 1.3 DNA Nanotechnology 6 1.3.1 Tile based assembly 9 1.3.2 DNA origami and single-stranded tiles 10 1.3.3 Some applications of DNA nanotechnology 12 1.3.4 Wireframe structures 15 1.3.5 Computational tools and DNA nanotechnology. 17 Chapter 2 Motivation and objectives 19 Chapter 3 Design and Synthesis of Triangulated DNA Origami Trusses 20 3.1 Introduction 20 3.2 Results and Discussion 21 3.2.1 Design 21 3.2.2 Nomenclature and parameters of the tube structures 23 3.2.3 Gel electrophoreses analysis 25 3.2.4 Imaging of the purified structures 26 3.2.5 Optimizing the folding conditions 28 3.2.6 Comparison to vHelix 29 3.3 Conclusions 29 3.4 Methods 30 3.4.1 Standard DNA origami assembly reaction. 30 3.4.2 Gel purification. 30 3.4.3 AFM sample preparation. 31 3.4.4 TEM sample preparation. 31 3.4.5 Instructions for mixing the staple sets. 31 Chapter 4 Triangulated wireframe structures assembled using single-stranded DNA tiles 33 4.1 Introduction 33 4.2 Results and Discussion 35 4.2.1 Designing the structures 35 4.2.2 Synthesis of test structures 37 4.2.3 Molecular dynamics simulations of 6-arm junctions 38 4.2.4 Assembly of the finite structures 40 4.2.5 Influence of salt concentration and folding times 42 4.2.6 Molecular dynamics simulations of the rhombus structure 43 4.2.7 1D SST crystals 44 4.2.8 Controlling the crystal growth 46 4.3 Conclusions 48 4.4 Methods 49 4.4.1 SST Folding 49 4.4.2 Agarose Gel Electrophoresis 49 4.4.3 tSEM Characterization 49 4.4.4 AFM Imaging 49 4.4.5 AGE-Based Folding-Yield Estimation 49 4.4.6 Molecular Dynamics Simulations 50 Chapter 5 Structural transformation of wireframe DNA origami via DNA polymerase assisted gap-filling 52 5.1 Introduction 52 5.2 Results and Discussion 54 5.2.1 Design of the Structures 54 5.2.2 Folding of Gap-Structures 56 5.2.3 Inactivation of Polymerase. 57 5.2.4 Secondary Structures. 58 5.2.5 Folding Kinetics of Gap Origami. 60 5.3 Conclusions 61 5.4 Methods 62 5.4.1 DNA origami folding 62 5.4.2 Gap filling of the wireframe DNA origami structures 63 5.4.3 Agarose gel electrophoresis 63 5.4.4 PAGE gel analysis 63 5.4.5 tSEM characterization 64 5.4.6 AFM imaging 64 5.4.7 AGE based folding-yield estimation 64 5.4.8 Gibbs free energy simulation using mfold 65 5.4.9 List of sequence for folding the DNA origami triangulated structures 65 Chapter 6 Summary and outlook 67 Appendix 69 A.1 Additional figures from chapter 369 A.2 Additional figures from chapter 4 77 A.3 Additional figures from chapter 5 111 Bibliography 127 Erklärung 138

The aim of this thesis is to show how to design a commercially successful website and what are the possibilities of its succesive promotion. First a user research and analysis of competition is done and on the basis of this a new website is designed and created. After its implementation this site is then supported by several online marketing tools.

This diploma thesis describes both the theoretical and practical part of the process of design and implementation of interactive computer software for the~management of~the~quality control of the~devices, assigned by SIGMA GROUP a.s., more precisely its Energo division, which is engaged in the maintenance and repair of pumping equipment. The theoretical part explains the concept of user experience, its acquisition methods, specification of~requirements and interface design methods. The next part is focused on the description of the~formats used by common text editors. Within the theoretical part is described the design of the interactive application itself. In the practical part there is a description of the main parts of the system and the conclusions from the system testing.