The processes influencing larval fish survival in the low-latitude open ocean are poorly understood, especially with regard to feeding. As part of a large-scale study that included two years of monthly sampling in the Straits of Florida (SOF), the objectives of this dissertation were to elucidate the larval fish feeding behaviors and strategies of 1) istiophorid billfishes, 2) tunas, and 3) coral reef fishes, while also 4) characterizing the feeding environment, synthesizing the dominant trophic pathways to fish larvae, and reviewing the literature for evidence of latitudinal distinctions in larval fish trophodynamics. Larval billfishes exhibited highly selective feeding, and their diets were numerically dominated (90%) by two genera of crustaceans, Farranula copepods and Evadne cladocerans. These prey were consumed throughout early larval ontogeny, from first-feeding through piscivorous lengths (> 5 mm), until piscivory became exclusive near 12 mm. High feeding incidence (0.94) and rapid digestion (~3.5 hrs) suggests frequent and successful feeding by billfish larvae. For tunas, nearly all larvae examined (>98%) contained prey. Thunnus spp. exhibited a mixed diet, while skipjack, little tunny, and Auxis spp. nearly exclusively consumed appendicularians. All four tuna taxa co-occurred in the western SOF where prey was more abundant, while in the central and eastern SOF (where prey availability was lower), only Thunnus spp. and skipjack were present. Additionally, these two taxa exhibited significantly different vertical distributions. Estimates of predatory impact indicated the potential for depletion of resources in the absence of the spatial and dietary niches of larval tunas. Coral reef fish families examined included Serranidae, Lutjanidae, Mullidae, Pomacentridae, Labridae, Scaridae, and Acanthuridae. Feeding incidences were high (0.94 to 1.0) for all taxa except scarids (0.04), and diets were narrow and predator-specific. Cluster analysis yielded clear groupings based on the selective feeding exhibited by the taxa, while within taxa, canonical correspondence analysis illustrated the change in diet with a variety of variables. The physical and biological environment varied markedly across the SOF, largely influenced by the Florida Current. Characteristics examined included thermocline depth, fluorescence, and abundances of total plankton and copepod nauplii. The feeding ecologies of the 21 taxa of fish larvae in this work were synthesized into qualitative and quantitative webs that illustrate the variable trophodynamic strategies of larvae in the SOF and the levels of community reliance upon zooplankton prey types. A review of 170 investigations on larval fish feeding revealed notable distinctions between high- and low-latitude regions, highlighting the substantial variability across environments in the role of larval fishes within the planktonic food web.

Bacteria, nanoflagellates and ciliates constitute the microbial loop and it is a model of the pathways of carbon and nutrient cycling through microbial components of pelagic aquatic communities. The current study comprised of a comparative study of the microbial food web community along north to south latitudinal gradient using space for time susbtitute, monitoring and mesocosms experiments with contrasting nutrient and predation states. We investigated effect of fish predation through different zooplankton taxa on microbial loop community with in situ food web experiments in 14 lakes along north to south latitudinal gradient. The effect of seasonality was also determined by monitoring in Lakes Eymir and Mogan between 2010 and 2011. Effects of hydrology and fish through microbial community was studied in mesocosms in Lake Eymir. An implication of global warming along with eutrophication on microbial community was further explored in warmed and nutrient enriched artificial ponds during 4 months in Silkeborg, Denmark. Our results revealed that temperature, hydrology, fish, macrophytes and seasonality affected the top down control of zooplankton and bottom up control of nutrients on microbial loop and interactions between controls and increase in these controls had a strong negative impact on the contribution and biomass of microbial loop and change the interactions within microbial community. Global warming may also effect the impact of top down and bottom up controls through increasing eutrophication, temperature, change in hydrology and zooplankton composition and in a consequence of that efficiency of microbial loop may decrease in the future warmer, drier and eutrophic conditions.

This study combines Optical Plankton Counter data on zooplankton abundance and Acoustic Doppler Current Profiler data on water currents to test hypotheses about spatial zooplankton distributions near a major point of land affecting local wind and water current patterns. Data were collected by repeatedly sampling a 2 km linear transect in the South Arm of Lake Opeongo, Ontario, Canada during July, 2008. Moving Split Window techniques were used to identify breakpoints in both zooplankton biomass concentration and a measure of water turbulence, and confirmed an effect of the topographic feature on local zooplankton distributions. Using additional data collected in 2001 and 2003, zooplankton abundance distributions were also tested under three wind speed ranges predicted from physical principles to correspond to varying stability of the water column. Significant differences were found in the variability and patch sizes of the distributions, indicating the importance of wind speeds on zooplankton patchiness.

This study combines Optical Plankton Counter data on zooplankton abundance and Acoustic Doppler Current Profiler data on water currents to test hypotheses about spatial zooplankton distributions near a major point of land affecting local wind and water current patterns. Data were collected by repeatedly sampling a 2 km linear transect in the South Arm of Lake Opeongo, Ontario, Canada during July, 2008. Moving Split Window techniques were used to identify breakpoints in both zooplankton biomass concentration and a measure of water turbulence, and confirmed an effect of the topographic feature on local zooplankton distributions. Using additional data collected in 2001 and 2003, zooplankton abundance distributions were also tested under three wind speed ranges predicted from physical principles to correspond to varying stability of the water column. Significant differences were found in the variability and patch sizes of the distributions, indicating the importance of wind speeds on zooplankton patchiness.

Inflows affect water quality, food web dynamics, and even the incidence of harmful algal blooms. It may be that inflows can be manipulated to create refuge habitat for biota trying to escape poor environmental conditions, such as fish populations in lakes during times of toxic Prymnesium parvum blooms. Water availability sometimes can be an issue, especially in arid climates, which limits this approach to management. Utilizing source water from deeper depths to displace surface waters, however, might effectively mimic inflow events. I test this notion by conducting in-lake mesocosm experiments with natural plankton communities where I manipulate hydraulic flushing. Results show that P. parvum cell density is reduced by 69%, and ambient toxicity completely ameliorates during pre-bloom conditions in the lake. During conditions of bloom development, population density is reduced by 53%, toxicity by 57%, and bloom proportions are never reached. There is minimal effect of these inflows on total phytoplankton and zooplankton biomass, and little effect on water quality. Shifts toward more rapidly growing phytoplankton taxa are observed, as are enhanced copepod nauplii. In other words, while inflows using deep waters suppress P. parvum bloom initiation and development, they are benign to other aspects of the lower food web and environment. The results from using deep lake water to suppress harmful algal blooms indicate this may be a promising management approach and further studies are recommended to test whether this mitigating effect can translate to a large-scale in-lake treatment.

In this study, the hydrology and physical, chemical and biological variables of a shallow lake system including the Lakes Mogan and Eymir between 1997-2005 were evaluated. In Lake Eymir, a biomanipulation study was conducted between August, 1998 &ndash / December, 1999. Upon biomanipulation, Lake Eymir shifted to clearwater state with submerged vegetation domination during 2000-2003. However, in 2004, the lake shifted back to algae-dominated turbid state since the buffer mechanisms provided by submerged plants were absent. In the summer of 2005, fish kills were observed due to algal bloom. However, due to increasing hydraulic residence time in the lake, internal processes became more important for nutrients. Lake Mogan faces seasonal and interannual water level fluctuations. During the low water levels experienced in 2001 and 2005, which coincided with the high hydraulic residence times, the in-lake phosphorus amount was controlled by internal processes rather than external loading. Moreover, results revealed that hydrology and submerged plants were important in the ecology of Lake Mogan. Furthermore, the relationship between the phytoplankton, zooplankton and the environment in Lakes Eymir and Mogan, which was predicted via Canonical Corresponding Analysis, revealed that nutrients and water transparency were both important for plankton communities. Both the top-down and bottom up effects were valid in Lake Eymir, while only the bottom-up effect and submerged plants were important for Lake Mogan. Finally, the present study provided a good example for the submerged plant dominated clearwater state triggered by biomanipulation, and the impact of hydrology on the ecology of shallow lakes.

In this study, annual variations in biochemical composition of seston and zooplankton community were investigated to characterize the nutritional environment of zooplankton in the Mersin Bay, NE Mediterranean Sea. For this goal, seawater and zooplankton samples were collected at monthly intervals from two stations / one representing coastal and other representing open waters characteristics from November 2004 to January 2006. Seawater samples were collected with Niskin bottles from the sea surface. Zooplankton samples were collected both in the horizontal and vertical plane by towing a Nansen net (70 cm mouth diameter with 112 &micro / m mesh). Surface seston chl-a, lipid, protein and carbohydrate concentrations were measured by fractionating seawater into three different size groups, 0.7-2.7, 2.7-18 and &gt / 18 &micro / m representing pico, nano and micro particulates in the seston. Zooplankton biomass and abundance were determined at four size fractions: 112-200, 200-500, 500-1000 and &gt / 1000 &micro / m / dry and organic weights were measured by gravimetric method and major taxonomic groups of zooplankton was identified under stereo-microscope. The nearshore station was always more productive than the offshore station in terms of chl-a, particulate organic matter (POM: protein+lipid+carbohydrate), zooplankton abundance and biomass. Chl-a maxima occured in spring and autumn at both stations. Very low chl-a concentrations at the offshore station (0.02-0.35 &micro / g L-1) confirmed oligotrophic character of the Northeastern Mediterranean. The highest chl-a concentration (2.4 &micro / g L-1) was observed in March 2005 at the nearshore station due to the input of Lamas River nearby. POM varied from 42.1 &micro / g L-1 (in January 2006) to 1082 &micro / g L-1 (in March 2005) and 53.7 &micro / g L-1 (in January 2006) to 246 &micro / g L-1 (in May 2005) at the nearshore and offshore stations, respectively. The oligotrophy of this system was indicated by the extremely low particulate lipid, protein and carbohydrate concentrations (1-3 times lower than in more productive systems). The most evident characteristic of this oligotrophic environment was the dominance of pico-POM throughout the study period, accounting for 31&ndash / 65 % of the total carbohydrates, proteins, lipids and chl-a. The prt:cho ratio was generally lower than 1 (low in organic nitrogen). Carbohydrate was the dominant biochemical component at both stations. Zooplankton varied during the sampling period, and they showed two peak abundances, in spring and autumn, with small increase in summer. The higher biomasses of zooplankton were observed in summer and autumn in the entire water column, but in spring and autumn periods in the surface water. Zooplankton data showed that 200-500 and 112-200 &micro / m size fractions were dominant in abundance at both stations. However, 200-500 &micro / m size fraction was dominant in zooplankton biomass at nearshore, whereas &gt / 1000 &micro / m size fraction was at offshore station. Copepods were the most abundant zooplankton group and dominated the distribution of total zooplankton, followed by crustace nauplii, appendicularia, cladocera and pteropoda.

Locomotion is a key characteristic of almost all forms of life and is often accomplished, whether on land, in water, or in the air, by reciprocal motion of two or more appendages. Among the zooplankton, many species propel themselves by rhythmically beating multiple pairs of closely spaced leg-like appendages in a back-to-front (metachronal) pattern. The focus of this study is to understand the mechanical design, kinematic operation, and hydrodynamic result of metachrony in the zooplankton. In the first part of this study, Antarctic krill (Euphausia superba) are investigated as an ecologically important model species that metachronally beats its swimming legs (pleopods) to perform drag-based propulsion. Based on high speed videos of freely swimming Antarctic krill, hovering, fast forward swimming, and upside down swimming are identified as three distinct swimming modes with significantly different stroke amplitudes and beat frequencies. When transitioning between hovering and fast forward swimming, Antarctic krill first increase beat amplitude and secondarily increase beat frequency. In considering the design components that contribute to metachrony being a successful swimming technique, a comparison among many different species shows that the ratio between the appendage separation distance and appendage length is limited to a narrow range of values (i.e. 0.2 - 0.65). In the second part of this study, metachrony is examined at smaller length and time scales by examining the impulsive escape jump of a calanoid copepod (Calanus finmarchicus). The wake generated by the copepod's metachronally beating swimming legs is experimentally measured using a novel (and newly developed) tomographic particle image velocimetry (PIV) system capable of making volumetric 3D velocity measurements with high temporal and spatial resolution using IR illumination. The flow generated by the escaping copepod consisted of a stronger posterior vortex ring generated by the metachronally stroking swimming legs and a weaker one generated anteriorly around the body by the impulsive start of the escape, both of which decayed over time. The experiments also revealed azimuthal asymmetry in the vortices caused by body yawing and the action of the swimming legs, flow features not considered in previous axisymmetric computational and theoretical models of copepod jumps. While not accounting for this asymmetry, an impulsive stresslet is nonetheless useful in modeling the flow created by the escaping copepod and represents the flow more accurately than an impulsive Stokeslet. In the final part of this study, the flow associated with metachronal hovering in Antarctic krill is experimentally and theoretically investigated in regards to the energy requirements of the pelagic lifestyle. Volumetric flow measurements of a hovering Antarctic krill show that each stroking pleopod drags flow behind it such that a downward stream develops medially. The lateral exopodites induce tip vortices which add to the lift force on each appendage. Furthermore, the flow beneath the hovering krill develops into a pulsed jet with a Strouhal number in the 'high-efficiency zone' of 0.2 < St < 0.4. Actuator disk theory is used to make theoretical estimates of the induced power necessary to hover, the results of which match induced power values calculated from measured flow gradients contributing to viscous energy dissipation.

The focus of my thesis research is on the interplay between individual behavior, population dynamics and community-level processes within zooplankton communities in coastal Maine. The target organisms of my thesis work are marine copepods. Copepods are small (1-10 mm) crustaceans that perform the essential ecosystem function of consuming and assimilating primary production (phytoplankton) making it available to higher trophic levels such as commercially important fishes. Therefore, copepod population growth is of critical importance to marine food webs. Fertilization limitation has been suggested as a constraint on copepod population growth but field surveys describing the prevalence of fertilization limitation are lacking. During my doctoral research, I explored the in situ fertilization success of two marine copepod species, Temora longicornis and Eurytemora herdmani in coastal Maine. I collected monthly zooplankton samples and analyzed clutches from field-caught females using an egg-staining technique. My results indicate that both species exhibit fertilization limitation in nature and the factors correlated with their fertilization span population, community and ecosystem level factors. To determine a causal relationship between predator density and copepod mating success, I conducted laboratory experiments to assess the effects of a common mysid shrimp predator, Neomysis americana on Eurytemora herdmani mating success. I subjected males and females to predators or predator cues. I found that the presence of a mysid predator, or only a predator cue, reduced copulation frequency and spermatophore transfer leading to a 38-61% decrease in E. herdmani nauplii production. These results suggest that mysid predators can constrain copepod population growth through non-consumptive processes. To determine the effects that resources can impose on copepod behavior, I explored the behavioral and fitness consequences of Temora longicornis ingesting Alexandrium fundyense, a phytoplankton species that forms harmful algal blooms in coastal Maine. My results suggest that ingesting A. fundyense causes copepods to swim faster and with more directional persistence compared to control algae. Temora longicornis increased their average swimming velocity by 24%, which leads to a 24-54% increase in their theoretical encounter rate with predators. Therefore, these findings suggest behaviorally mediated copepod-algal interactions may have significant impacts on harmful algal bloom dynamics and the fate of toxins in marine food webs.

Thesis (Ph. D.)--Lehigh University, 2002. / Includes vita. Includes bibliographical references (leaves 121-132).