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Previous issue date: 2012 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Introdução: Ha um interesse cientifico crescente na plasticidade e potencial terapeutico das celulas-tronco do tecido adiposo humano (CTTAH), celulas multipotentes e abundantes no tecido adiposo que podem se diferenciar in vitro em multiplas linhagens celulares, incluindo adipocitos, condrocitos, osteoblastos, celulas neurais, endoteliais e cardiomiocitos. O objetivo deste estudo foi verificar o efeito da AII nas CTTAH. Metodos: Celulas precursoras humanas do tecido adiposo foram obtidas de tecido abdominal subcutaneo, separadas por gradiente de centrifugacao por densidade, cultivadas, estimulados com diferentes meios de cultivo e entao analisadas. Resultados: Os resultados de citometria de fluxo evidenciaram a expressao dos marcadores CD73, CD90 e CD105, contrastando com a falta de expressao dos marcadores CD16, CD34 e CD45. A diferenciacao adipogenica, osteogenica e condrogenica tambem foi induzida, confirmando seu potencial de celulas-tronco mesenquimais in vitro As CTTAH foram capazes de se replicar em cultivo mantendo o fenotipo semelhante a fibroblastos in vitro. Quando estimuladas com os diferentes meios de cultivo verificamos que os meios contendo AII aumentaram a atividade celular e mitotica dessas celulas. Conclusao: Com a identificacao da expressao dos receptores AT1 e AT2 e sob acao da AII exogena verificou-se o aumento da atividade celular e mitotica das CTTAH / Introduction: There is a crescent scientific interest about the plasticity and
therapeutic potential of adipose-derived stem cells (ASCs), multipotent and
abundant cells in adipose tissue, which are able to differentiate in multiple
cellular lineages in vitro, including adipocytes, chondrocytes, osteoblasts,
neural cells, endothelial and cardiomyocytes. Objective: The aim of this
study was verify the effect of AII on ASCs. Methods: Human adipose
tissue precursor cells were obtained of subcutaneous abdominal tissue,
separated by density centrifugation gradient, cultivated and stimulated with
different culture media and, then analysed. Results: The results of flow
citometry showed the expression of CD73, CD90 and CD105 markers,
constrasting with the lack of expression of CD16, CD34 and CD45
markers. The adipogenic, osteogenic and chondrogenic differentiation were
also induced, confirming its mesenchymal stem cell potential in vitro. The
human ASCs were able to replicate in culture, keeping phenotype similar to
fibroblasts in vitro. When stimulated with different culture media, we
verified that the media containing AII increased the cellular and mitotic
activity of these cells. Conclusion: With the identification of the
expression of the AT1 e AT2 receptors and under AII exogenous action, it
was verified the increase of cellular activity and mitotic of human ASCs. / BV UNIFESP: Teses e dissertações
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.unifesp.br:11600/22247 |
| Date | January 2012 |
| Creators | Gaiba, Silvana [UNIFESP] |
| Contributors | Universidade Federal de São Paulo (UNIFESP), Ferreira, Lydia Masako [UNIFESP] |
| Publisher | Universidade Federal de São Paulo (UNIFESP) |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | 125 f. |
| Source | reponame:Repositório Institucional da UNIFESP, instname:Universidade Federal de São Paulo, instacron:UNIFESP |
| Rights | info:eu-repo/semantics/openAccess |
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