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Previous issue date: 2013-11-01 / The aims of this study were: (a) establish, through a review of the literature, the state of the art regarding the use of photodynamic therapy for the disinfection of the root canal system as well as future prospects, (b) evaluate the periapical tissues response on rats teeth with apical periodontitis, to photodynamic therapy as an adjunct to endodontic treatment through histological, microbiological and radiological analysis. Therefore, 32 left lower rats molars were exposed to the oral environment for 21 days to induce apical periodontitis. The animals were randomly divided into four groups according to proposed treatment: endodontic treatment in one session (G1); endodontic treatment in two sessions with with calcium hydroxide [Ca(OH)2] dressing for 14 days (G2) ; endodontic treatment in one session associated with photodynamic therapy (PDT) (G3) and endodontic treatment in two sessions with Ca(OH)2 for 14 days, associated with PDT (G4). After 28 days, the animals were killed and the jaws prepared for evaluation. For microbiological analysis samples were collected before and after each treatment step. Quantification of CFU/mL were analyzed using linear mixed models, showing significant microbial reduction after all proposed treatments, compared to the initial collection, although no significant difference between groups were found. The radiographic results were submitted to ANOVA, showing no statistically significant difference between groups. For histological data, Kruskal-Wallis test were used, followed by Mann-Whitney U test for those cases where differences were detected. These differences were found in neutrophils and eosinophils populations in groups where PDT was used and macrophages and giant cells when Ca(OH)2 was used, with or without PDT. These data seem to point to a better repair quality when PDT is applied, caused by laser biomodulator effect in periapical tissues. The association of PDT with an interappointment dressing with Ca(OH)2 produces better conditions to stimulate repair, being more research needed to determine this relationship. / Os objetivos deste estudo foram: (a) estabelecer, atrav?s de revis?o da literatura, o estado da arte na aplicabilidade da terapia fotodin?mica para a desinfec??o do sistema de canais radiculares bem como as perspectivas futuras; (b) avaliar a resposta dos tecidos periapicais de dentes de ratos, portadores de periodontite apical, ? terapia fotodin?mica como coadjuvante ao tratamento endod?ntico, atrav?s de an?lise histol?gica, microbiol?gica e radiogr?fica. Para tanto, 32 molares inferiores esquerdos de ratos foram expostos ao meio bucal por 21 dias, para indu??o da periodontite apical. Ap?s, foram divididos aleatoriamente em quatro grupos conforme o tratamento proposto: tratamento endod?ntico em sess?o ?nica (G1); tratamento endod?ntico em duas sess?es com medica??o intracanal ? base de hidr?xido de c?lcio [Ca(OH)2] por 14 dias (G2); tratamento endod?ntico em sess?o ?nica, associado ? terapia fotodin?mica (PDT) (G3) e tratamento endod?ntico em duas sess?es com Ca(OH)2 por 14 dias, associado ? PDT (G4). Ap?s 28 dias, os animais foram mortos e as mand?bulas preparadas para avalia??o. Para avalia??o microbiol?gica foram realizadas coletas antes e ap?s cada etapa de tratamento. As quantifica??es de UFC/mL foram analisadas atrav?s de modelos mistos lineares, mostrando significativa redu??o microbiana ap?s todos os tratamentos propostos, quando comparados ? coleta inicial, n?o havendo por?m diferen?a significativa entre os grupos. Os resultados radiogr?ficos foram submetidos a ANOVA, n?o havendo diferen?a estatisticamente significativas entre os grupos. Para os dados histol?gicos, foi utilizado o teste de Kruskall-Wallis seguido por Mann-Whitney U para aqueles casos onde diferen?as foram detectadas. Estas diferen?as foram encontradas nas popula??es de neutr?filos e eosin?filos nos grupos onde a PDT foi utilizada e para macr?fagos e c?lulas gigantes quando o Ca(OH)2 foi utilizado, independentemente da PDT. Os dados parecem apontar para uma melhor condi??o de reparo quando da utiliza??o da PDT, pelo efeito biomodulador do laser nos tecidos periapicais. A associa??o da PDT com MIC com Ca(OH)2 parece propiciar os melhores resultados, sendo necess?rios mais estudos para determinar esta rela??o.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/1230 |
| Date | 01 November 2013 |
| Creators | Trindade, Alessandra Cesar |
| Contributors | Weber, Jo?o Batista Blessmann |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Odontologia, PUCRS, BR, Faculdade de Odontologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -8096554818733665164, 500, 600, 4673435736271820140 |
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