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Previous issue date: 2015 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (Capes) / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Funda??o de Amparo ? Pesquisa do estado de Minas Gerais (FAPEMIG) / As plantas medicinais s?o fontes promissoras de novas drogas, em que a regi?o do
Cerrado se destaca pela sua vasta biodiversidade. Dentre as plantas medicinais
utilizadas nesta regi?o se encontra a esp?cie Miconia ferruginata DC. que ? conhecida
como pixirica-do-campo ou babaten?o. Esta esp?cie ? utilizada no tratamento de
doen?as de pele, e outras de origens inflamat?rias, parasit?rias e infecciosas, por?m os
estudos qu?micos s?o escassos. Assim, os objetivos deste trabalho foram avaliar o perfil
qu?mico e o potencial biol?gico da M. ferruginata (Melastomataceae). A esp?cie foi
coletada no munic?pio de Diamantina, MG do qual foram preparados os extratos
aquosos e etan?licos das folhas/flores e do caule. Por meio da triagem fitoqu?mica e das
an?lises de CLAE-DAD-EM obteve-se um perfil qu?mico rico em compostos fen?licos.
Foi poss?vel a identifica??o da presen?a de taninos, ?cidos fen?licos, flavonoides, e
derivados glicosilados da quercetina. Pela an?lise dos constituintes vol?teis por CG-EM
foi poss?vel identificar a presen?a de quatorze compostos, sendo eles pertencentes as
classes dos sesquiterpenos, hidrocarbonetos, monoterpenos, fenilpropanoides e alco?is.
No qual os sesquiterpenos ?-cariofileno e ?-humuleno foram os compostos majorit?rios,
os quais s?o associados na literatura a diversas atividades biol?gicas, em especial a
a??es anti-inflamat?rias, antif?ngicas e antitumorais. A an?lise das fra??es por CG-EM
e CLAE-DAD foi poss?vel identificar catequinas, ?cido g?lico e flavon?is nos extratos
aquosos e ?steres graxos e as subclasses das flavonas e flavon?is nos extratos etan?licos.
Nas avalia??es biol?gicas os extratos etan?licos e aquosos apresentaram baixa
toxicidade em c?lulas de mam?feros, em que a concentra??o selecionada para a
avalia??o das demais atividades biol?gicas foi de at? 500 ?g/mL. Para a atividade
antitumoral, os extratos apresentaram um grande potencial concentra??o dependente,
com valores de IC50 variando de 56,44 a 180,4 ?g/mL. Bem como o tempo de exposi??o
aumentou a pot?ncia de todos os extratos. Tamb?m foi observado que os extratos
aquosos promoveram a inibi??o da prolifera??o de linf?citos estimulados por mit?genos,
apresentando uma efic?cia em rela??o ? dexametasona, de 39,94% para o extrato das
folhas a 45 ?g/mL, e de 57,3%, 137,4% e 251,8% para os extratos do caule a 15, 20 e
30 ?g/mL, respectivamente. Este efeito proliferativo pode estar relacionado ao potencial
anti-inflamat?rio da M. ferruginata, relatada na medicina popular. Possivelmente, as
atividades biol?gicas est?o relacionadas ao rico perfil qu?mico desta esp?cie,
principalmente em compostos fen?licos. Para as atividades antimicrobianas, tripanocida
9
e leishmanicida n?o foram observadas a inibi??o do crescimento ou toxicidade frentes
as cepas avaliadas. Desta forma este trabalho contribuiu para o conhecimento qu?mico e
biol?gico da M. ferruginata. Por?m ensaios futuros utilizando as fra??es e subst?ncias
isoladas, se fazem necess?rios para fortalecer os dados aqui apresentados e para
demonstrar quais os mecanismos moleculares envolvidos nas atividades biol?gicas investigadas. / Disserta??o (Mestrado) ? Programa de P?s-gradua??o em Ci?ncias Farmac?uticas, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2015. / ABSTRACT
Medicinal plants are reassuring sources of new drugs, in which the Cerrado region
stands out for its broad biodiversity. Among the medicinal plants used in this same
region is the species Miconia ferruginata DC. which is also known as pixirica-docampo
or babaten?o. This species is used in the treatment of skin diseases, and other
inflammatory, infectious and parasitic sources, despite the chemical studies about it are
lacking. Therefore, the objectives of this study were to evaluate the chemical profile and
biological potential of the M. ferruginata (Melastomataceae).The species was collected
in Diamantina, MG, in which it was prepared for water and ethanol extraction of the
leaves/flowers and stem. Through phytochemical screening and analysis of HPLC-MSDAD,
we obtained a rich chemical profile in phenolic compounds. The identification of
tannins, phenolic acids, flavonoids, glycosides of quercetin and derivativeshas been
detected. For the analysis of volatile constituents by GC-MS, it was possible to identify
the presence of fourteen compounds, namely belonging classes of sesquiterpenes,
hydrocarbons, monoterpenes, phenylpropanoids and alcohols. For that,? and?-
caryophyllene, humulenesesquiterpenes were the main components, which are
associated in the literature several biological activities, especially anti-inflammatory,
antifungal and antitumor activities.In the analysis of the fractions by GC-MS and
HPLC-DAD, we identified catechins, gallic acid and flavonols in aqueous
extracts,including fatty esters and subclass of flavones and flavonols in ethanol extracts.
Within the biological evaluations, aqueous and ethanolic extracts showed low toxicity
to mammalian cells, wherein the concentration selected for analysis of other biological
activities was up to 500 ?g/mL. For antitumor activity, the extracts showed a great
potential concentration-dependent, with IC50 values ranging from 56.44 to 180.4 ?g/mL.
Just as the exposure time increased the potency of all extracts, it was also observed that
aqueous extracts promoted inhibition of the proliferation of mitogen-stimulated
lymphocytes, showing a efficacy when compared to dexamethasone, 39.94% for the
extract from the leaves of 45 ?g/mL, and 57.3%, 137.4% and 251.8% for the extracts of
stem of 15.20 and 30 ?g/mL, respectively. This proliferative effect may be related to the
anti-inflammatory potential of M. ferruginata, reported in folk medicine. Possibly, the
biological activities are related to the rich chemical profile of this species, mainly in
phenolic compounds. For the antimicrobial, trypanocidal and leishmanicidal activities,
we have not observed growth inhibition or toxicity fronts of the evaluated strains. Thus
11
this work contributed to the chemical and biological knowledge of M. ferruginata.
Nevertheless, future trials using isolated fractions and substances are needed to
strengthen the data presented here and to demonstrate the molecular mechanisms
involved in the biological activities investigated. It is likely that the biological activities
are related to the rich chemical profile of this species, mainly regarding phenolic
compounds. For antimicrobial activity, trypanocidal and leishmanicidal, it was not
observed growth inhibition or toxicity regarding the evaluated strains. Thus, this work
contributed to the chemical and biological knowledge of the M. ferruginata. However,
future trials using isolated fractions and substances are needed to strengthen the data
presented here and to demonstrate the molecular mechanisms involved in the biological
activities hereby investigated.
| Identifer | oai:union.ndltd.org:IBICT/oai:acervo.ufvjm.edu.br/jspui:1/759 |
| Date | 24 April 2015 |
| Creators | Barroso, Poliana Ribeiro |
| Contributors | Greg?rio, Luiz El?dio, Martins, Helen Rodrigues, Santana, Marcos Aur?lio, Grael, Cristiane Fernanda Fuzer, Martins, Helen Rodrigues, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Greg?rio, Luiz El?dio |
| Publisher | UFVJM |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFVJM, instname:Universidade Federal dos Vales do Jequitinhonha e Mucuri, instacron:UFVJM |
| Rights | A concess?o da licen?a deste item refere-se ao ? termo de autoriza??o impresso assinado pelo autor, assim como na licen?a Creative Commons, com as seguintes condi??es: Na qualidade de titular dos direitos de autor da publica??o, autorizo a Universidade Federal dos Vales do Jequitinhonha e Mucuri e o IBICT a disponibilizar por meio de seus reposit?rios, sem ressarcimento dos direitos autorais, de acordo com a Lei n? 9610/98, o texto integral da obra disponibilizada, conforme permiss?es assinaladas, para fins de leitura, impress?o e/ou download, a t?tulo de divulga??o da produ??o cient?fica brasileira, e preserva??o, a partir desta data., info:eu-repo/semantics/openAccess |
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