Made available in DSpace on 2014-12-17T15:30:51Z (GMT). No. of bitstreams: 1
AnaRLA.pdf: 621369 bytes, checksum: 16e9cd521a5599eb0289fb550685807b (MD5)
Previous issue date: 2009-01-12 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Periodontal infections consist of a group of inflammatory conditions caused by microorganisms that colonize the tooth surface through the formation of dental biofilm. Chronic infections such as periodontitis have been associated to the development and progression of atherosclerosis. AIM: Detect cultivatable and non-cultivatable periodontopathogenic bacteria in atheromatous plaques; search for factors associated to the presence of these bacteria in the atheromatous plaques and characterize the presence of cultivatable and non-cultivatable bacteria in these plaques. METHODOLOGY: A cross-sectional study was performed with a sample of 30 patients diagnosed with atherosclerosis in the carotid, coronary or femoral arteries and surgically treated with angioplasty and stent implant, bypass or endarterectomy. The plaques were collected during surgery and analyzed using the PCR molecular technique for the presence of the DNA of the cultivatable bacteria Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Treponema denticola and of the non-cultivatable Synergistes phylotypes. The patients were examined in the infirmary, after the surgery, where they also responded to a questionnaire aimed at determining factors associated to the presence of periodontopathogenic bacteria in the atheromatous plaques. RESULTS: All patients with tooth (66,7%) possessed disease periodontal, being 95% severe and 65% widespread. No periodontopathogenic bacteria were found in the atheromatous plaques. However, four samples (13.3%) were positive for the presence of bacteria. Of these, three participants were dentate, being two carriers of widespread severe chronic periodontite and one of located severe chronic periodontitis. None of them told the accomplishment of procedures associated to possible bacteremia episodes, as treatment endodontic, extraction the last six months or some procedure surgical dental. CONCLUSION: The periodontopathogenic bacteria studied were not found in the atheromatous plaques, making it impossible to establish the prevalence of these pathogens or the factors associated to their presence in plaques, the detection of positive samples for bacteria suggests that other periodontal and non-periodontal pathogens be studied in an attempt at discovering the association or not between periodontal disease and/or others infections and atherosclerosis, from the presence of these bacteria in atheromas / As infec??es periodontais compreendem um grupo de condi??es inflamat?rias, causadas por microrganismos que colonizam a superf?cie dent?ria, atrav?s da forma??o do biofilme dent?rio. Atualmente, infec??es cr?nicas como as periodontais t?m sido associadas ao desenvolvimento e progress?o da aterosclerose. OBJETIVOS: Detectar bact?rias periodontopatog?nicas cultiv?veis e n?o cultiv?veis em placas ateromatosas; buscar fatores associados ? presen?a destas bact?rias nas placas ateromatosas e caracterizar a presen?a de bact?rias cultiv?veis e n?o cultiv?veis em placas ateromatosas. METODOLOGIA: Foi realizado um estudo seccional, utilizando uma amostra de 30 pacientes que apresentaram o diagn?stico de aterosclerose nas art?rias car?tidas, coronarianas ou femorais, e foram tratados cirurgicamente atrav?s da realiza??o dos procedimentos de angioplastia com implante de stent, bypasse ou endartarectomia. As placas foram coletadas durante as cirurgias realizadas e analisadas atrav?s da t?cnica molecular de PCR para a presen?a de DNA das bact?rias periodontais cultiv?veis Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis e Treponema denticola e o filotipo n?o-cultiv?vel Synergistes species. Os pacientes foram examinados no leito da enfermaria, ap?s as cirurgias, onde tamb?m responderam a um question?rio cujo objetivo foi buscar fatores associados ? presen?a de bact?rias periodontopatog?nicas nas placas ateromatosas. RESULTADOS: Todos os pacientes dentados (66,7%) possu?am doen?a periodontal, sendo 95% severa e 65% generalizada.Nenhuma bact?ria periodontopatog?nica pesquisada foi encontrada nas placas ateromatosas. No entanto, quatro amostras (13,3%) foram positivas para a presen?a de bact?rias. Destas, tr?s participantes eram dentados, sendo dois portadores de periodontite cr?nica severa generalizada e um de periodontite cr?nica severa localizada. Nenhum deles relatou a realiza??o de procedimentos associados a poss?veis epis?dios de bacteremia, como tratamento endod?ntico, exodontia nos ?ltimos seis meses ou algum procedimento cir?rgico odontol?gico. CONCLUS?O: As bact?rias periodontopatog?nicas estudadas n?o foram encontradas nas placas ateromatosas, n?o pudendo ser estabelecida a preval?ncia destes pat?genos e nem os fatores associados ? presen?a deles nas placas, mas, a detec??o das amostras positivas para bact?rias abre caminhos para que outros pat?genos periodontais e n?o periodontais sejam estudados na tentativa de elucidar de maneira definitiva a associa??o ou n?o entre a doen?a periodontal e/ou outras infec??es e a aterosclerose, atrav?s da presen?a destas bact?rias nos ateromas
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/17045 |
| Date | 12 January 2009 |
| Creators | Aquino, Ana Rafaela Luz de |
| Contributors | CPF:50337157472, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723244A9, Gurgel, Bruno C?sar de Vasconcelos, CPF:02569549410, http://lattes.cnpq.br/4649601602612601, Seabra, Eduardo Gomes, CPF:08610878449, http://lattes.cnpq.br/6218058757798526, Lima, Kenio Costa de |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Odontologia, UFRN, BR, Odontologia Preventiva e Social; Periodontia e Pr?tese Dent?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
Page generated in 0.0095 seconds