Bacterial bloodstream infections represent a severe and potentially life-threatening condition. Diagnostic tools, providing rapid species identification and antimicrobial susceptibility testing have a great impact on disease progression and treatment optimization. Typically, antimicrobial susceptibility testing involves a time-consuming process, and faster methods are likely to contribute in clinical settings. This study aimed to assess the reliability of the European Committee on Antimicrobial Susceptibility Testing (EUCAST) rapid antimicrobial susceptibility testing (RAST) with a 16-20-hour incubation period for implementation in a clinical microbiology laboratory. This method was conducted alongside the standardized disk diffusion method from EUCAST, encompassing all bacterial species compatible with 16-20-hour RAST. The method was executed 80 times, and over 450 readings were performed. It confirms EUCAST's method validation, affirming 16-20-hour RAST as a swift and precise method for blood bacterial resistance determination. All inhibition zones were readable, with 89% of antibiotics tested falling within 1 mm of the set target value. Notable deficiencies were observed in antibiotic-species combinations, such as trimethoprim/sulfamethoxazole and gentamicin against E. coli, and erythromycin against S. pneumoniae. Accurate interpretation of inhibition zones demands precise reading practices as some zones were ambiguous. The study also underscores the importance of overnight cultures in antimicrobial susceptibility testing via standardized disk diffusion, accentuating 16-20-hour RAST's pivotal role in potentially accelerating resistance determination by up to a day. Given its commendable performance, 16-20-hour RAST is ready to be incorporated into the standard procedures at clinical microbiology laboratories, as demonstrated by its successful adoption at Unilabs, Skövde.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:lnu-129835 |
| Date | January 2024 |
| Creators | Hörnell, Simon |
| Publisher | Linnéuniversitetet, Institutionen för kemi och biomedicin (KOB) |
| Source Sets | DiVA Archive at Upsalla University |
| Language | Swedish |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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