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Previous issue date: 2007-09-05 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / ?Fucans, sulfated polysaccharides extracted from brown algae and some echinoderms, have been extensively studied for its diverse biological activities and because of its interference with molecular mechanisms of cell to cell recognition, including leukocyte trafficking from blood vessels into sites of inflammation mediated by selectin, a family of adhesion molecules. In the present study, we examined structural features of a heterofucan extracted from brown algae Padina gymnospora and its effect on the leukocyte migration to the peritoneum. The sulfated polysaccharides were extracted from the brown seaweed by proteolysis with the proteolytic enzyme maxatase. The presence of protein and uronic acid contamination was detected in the crude polysaccharide extract. Fractionation of the crude extract with growing concentrations of acetone produced five fractions with different concentrations of fucose, xylose, uronic acid, galactose, glucose and sulfate. The fraction precipitated with 1.5 volumes of acetone was characterized by infrared and nuclear magnetic resonance, through which can be observed the presence of sulfate groups in the C4 of -L-fucose. The anti-inflammatory action of this composite was assessed by a sodium thioglycollate-induced peritonitis assay and through nitric oxide production by the peritoneal
macrophages using Griess reagent. Fraction F1.5 was efficient in reducing leukocyte influx into the peritoneal cavity when 10 mg/kg and 25mg/kg were used, resulting in a
decrease of 56 and 39%, respectively. A decrease of nitric oxide production occurred when high concentrations of fucana were used. The cytotoxicity of the composite was also assessed using the reduction of 3-(4,5 dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT). Fraction F1.5 had no cytotoxicity when 500 ?g/mL of the fraction was used. This study suggests the use of fraction F1.5 (heterofucan) as an anti-inflammatory / Fucanas, polissacar?deos sulfatados extra?dos de alga marrom e alguns
equinodermos, t?m sido extensivamente estudadas devido as suas diversas
atividades biol?gicas e pela sua interfer?ncia com mecanismos moleculares de
reconhecimento celular, incluindo o tr?fego de leuc?citos dos vasos sangu?neos para
o interior de s?tios inflamat?rios mediado pela fam?lia das mol?culas de ades?o
denominada selectinas. Neste presente estudo, n?s examinamos a estrutura de uma
heterofucana extra?da da alga marrom Padina gymnospora e seu efeito sobre a
migra??o de leuc?citos para o perit?nio. Os polissacar?deos sulfatados foram
extra?dos da alga marrom por prote?lise com a enzima proteol?tica maxatase. A
presen?a de contamina??o por prote?nas e ?cido ur?nico foi detectada no extrato
bruto dos polissacar?deos. Fracionamento do extrato bruto com concentra??es
crescentes de acetona produziu cinco fra??es com diferentes concentra??es de
fucose, xilose, ?cido ur?nico, galactose, glicose e sulfato. A fra??o precipitada com
1,5 volume de acetona foi caracterizada por infravermelho e resson?ncia magn?tica
nuclear, atrav?s das quais se p?de constatar a presen?a de grupos sulfato no C4 da
-L-fucose. A a??o antiinflamat?ria deste composto foi avaliada atrav?s do ensaio de
peritonite induzida por tioglicolato de s?dio e atrav?s da produ??o de ?xido n?trico
por macr?fagos peritoneais utilizando-se o reagente de Griess. A fra??o F1,5
mostrou-se eficiente na redu??o do influxo leucocit?rio para a cavidade peritoneal
quando utilizados 10 mg/Kg e 25 mg/Kg resultando em uma diminui??o na ordem de
56 e 39 %, respectivamente. Uma diminui??o na produ??o de ?xido n?trico ocorreu
quando altas concentra??es de fucana foram usadas. A citotoxicidade do composto
tamb?m foi avaliada utilizando-se como par?metro a redu??o do 3-(4,5-
dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). A fra??o F1,5 n?o
apresentou citotoxicidade quando utilizados 500 ?g/ml da fra??o. Este estudo sugere
o uso da fra??o F1,5 (heterofucana) como um antiinflamat?rio
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/18531 |
| Date | 05 September 2007 |
| Creators | Marques, Cybelle Teixeira |
| Contributors | CPF:06341462468, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783254U6&dataRevisao=null, Chavante, Suely Ferreira, CPF:33615217420, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786250T8, Leite, Edda Lisboa |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Bioqu?mica, UFRN, BR, Bioqu?mica; Biologia Molecular |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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