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Previous issue date: 2015-12-15 / As doen?as inflamat?rias intestinais abrangem um conjunto de desordens cr?nicasinflamat?rias, entre elas a colite ulcerativa (CU). O tratamento da CU se baseia no usode anti-inflamat?rios, por?m estes f?rmacos podem apresentam efeitos colaterais, o queestimula a busca de novas terapias. O objetivo desse trabalho foi avaliar o efeito doextrato hidroalco?lico de Turnera subulata na colite ulcerativa aguda induzida por ?cidoac?tico em ratos. CU foi induzida em ratos Wistar com a instila??o de 1ml de ?cidoac?tico 4% via retal. 42 animais foram distribu?dos em 6 grupos experimentais: GrupoControle, Grupo Col?tico, Grupo Sulfasalazina 500mg/Kg/dia (SSZ), Grupo T. subulata50mg/Kg/dia (TS 50mg), Grupo T. subulata 100mg/Kg/dia (TS 100mg), Grupo T.subulata 200mg/Kg/dia (TS 200mg). Durante o experimento foram avaliadosdiariamente, o consumo alimentar, ingest?o h?drica e peso dos animais. Os animaisforam eutanasiados e o col?n foi exposto para an?lise macrosc?pica. Fragmentos doc?lon foram utilizados para an?lise microsc?pica e avalia??o do estresse oxidativo. Adiferen?a entre as m?dias foi analisada por ANOVA a um n?vel de signific?ncia de 5%(p<0,05) com o aux?lio do Software GraphPad Prism. A an?lise dos resultadosdemonstrou que o Grupo Sulfassalazina teve a maior perda de peso ao longo doexperimento, 14,78% e o menor consumo alimentar de 6,23g de ra??o ao dia, os grupostratados com o extrato de T. subulata n?o apresentaram perda de peso significativaquando comparados com o Grupo Controle. O Grupo Col?tico apresentou o maiorescore macrosc?pico 6,5; enquanto o Grupo TS 50mg teve um menor escore 1,0. Aavalia??o microsc?pica mostrou a presen?a de edema, hemorragia, ulcera??o em todosos grupos experimentais, exceto o Controle. No entanto, o Grupo TS 50mg apresentou oprocesso inflamat?rio em menor intensidade. A avalia??o do estresse oxidativo revelouque os grupos tratados com o extrato de T. subulata modularam a atividade das enzimascatalase e super?xido desmutase. Observou-se ainda redu??o na peroxida??o lip?dica eprot?ica. Assim, ? poss?vel concluir que o extrato hidroalc?olico de T. subulata tevea??o anti- inflamat?ria e antioxidante na colite ulcerativa experimental. / Inflammatory bowel diseases is composed by a set of chronic and
inflammatory disorders, among them is ulcerative colitis (UC). UC treatment is
based on anti-inflammatory administration; however, this group of drugs
clearly leads to development of undesirable side effects, what stimulate the
search for new therapies alternatives. The aim of this study was to evaluate
the effect of hydroalcholic Turnera subulata extract on acetic acid-induced
acute UC in rats. UC was induced by 1 mL injection of 4% acetic acid via
rectal in Wistar mouse. 42 animals were distributed among 6 experimental
groups: Control, UC, Sulfasalazine 500 mg/Kg/day (SSZ), T. subulata
50mg/Kg/day (TS 50), T. subulata 100mg/Kg/day (TS 100), T. subulata
200mg/Kg/day (TS 200). Throughout the experiment, body weight, food and
water ingestion was daily evaluated. At the end of the experiment, the animals
were euthanized and a colon fragment was observed by macroscopic
analysis. Colon fragments were also collected for microscopic analysis and
oxidative stress evaluation. The means from each group was compared by
ANOVA test with a significance level of 5% (p<0.05) using GraphPad Prism
Software. As results, we can clearly observe that SSZ group had the greater
body weight decrease among the groups throughout the experiments, 14.78%,
as well as, the lowest food intake, 6.23 g of food/day. The animals treated with
T. subulata extracts showed no important body weight loss when compared to
control. UC group showed the highest tissue damage macroscope score, 6.5,
while TS 50 showed the lowest tissue damage score: 1. Microscope
evaluation showed the presence of edema, haemorraghia and ulceration in all
group of animals, except for Control. Nevertheless, TS 50 showed the lowest
inflammatory damage among all groups. Oxidative stress analysis revealed
that T. subulata treatment modulate catalase and superoxide dismutase
activity, we also observed a decrease in protein and lipid peroxidation in
response to extract administration. Taken together, these results shows that T.
subulata extract exerts anti-inflammatory and anti-oxidant effects on
experimental UC.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/21123 |
| Date | 15 December 2015 |
| Creators | Amarante, Maria do Socorro Medeiros |
| Contributors | 52904911049, Langassner, Silvana Maria Zucolotto, 81995725072, http://lattes.cnpq.br/7390416147619446, Vieira, Jeymesson Raphael Cardoso, 02930177438, http://lattes.cnpq.br/6727125662817949, Camillo, Christina da Silva |
| Publisher | Universidade Federal do Rio Grande do Norte, PROGRAMA DE P?S-GRADUA??O EM BIOLOGIA ESTRUTURAL E FUNCIONAL, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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