Orientador: Alba Regina Monteiro Souza Brito / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-15T09:46:43Z (GMT). No. of bitstreams: 1
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Previous issue date: 2010 / Abstract: Abarema cochliacarpos (Gomes) Barneby & Grimes, which belongs the Mimosaceae family, is popularly known as "barbatimao" by the tradicional communities in Northeastern Brazil that use its bark in the treatment of gastric ulcer and inflammation, among other uses. In this work, the antiulcerogenic activity of its extracts and fractions was screened, using the gastric ulcer model induced by absolute ethanol in rats and selecting of the butanolic fraction of the methanolic extracts at dosis of 150 mg/kg as being in the most effective one. The action mechanisms of this fraction were studied based on the mucus gastric content and gastric secretion models. We have also decided to test it in anti-inflammatory intestinal activity model. Acute toxicity of AC150 was evaluated in Swiss mice, and no symptoms or visible toxicity signs were observed. Wistar rats were used in the others experiments. The intraduodenal pathway was utilized only in the pylorus ligature model; in the other models the oral pathway was employed. The AC 150 was able to significantly increased the mucus production, as well as to change the biochemical parameters of the acid gastric secretion, reducing the gastric volume and increasing the pH, suggesting a cytoprotection and anti-secretory activity. AC150 showed healing activity in the chronic model of gastric ulcer induced by 30 % acetic acid. This was demonstrated by the reduction of lesion area, in agreement with HE histological analysis. The induction of cellular proliferation was indicated by the PCNA results. The immunolocation of HSP-70, COX-2 and VEGF in the gastric tissue suggested that the ulcer cicatrization activity was induced by the increased of the expression of these factors, as well as the increase of the mucus production highlightened in the PAS results. In the acute ulcerative colitis model induced by intra-colonic administration of trinitrobenzensulfonic acid (TNBS) (10 mg/animal) AC 100 and 150 mg/kg were able to significantly decrease the lesion and the mieloperoxidase (MPO) levels in the colonic mucosa. However, only AC150 decreased the levels of the tumour necrosis factor-alpha (TNF-a). The western blot analysis showed that AC 150 mg/kg induced the down-regulation of both cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) expression, and the involvement of signaling also demonstrated a reduction in the JNK activation in this dosis. In the chronic colitis model (TNBS, 30 mg/animal) AC 150 showed the same inhibition profile of the inflammatory parameters; nevertheless, the increase of the antiinflammatory cytokine production IL-10 was highlightened only in this model. These data suggested a significant protective effect of the chronic administration of AC during the chronic phase of the inflammatory response associated with TNBS induced colitis. Phytochemical studies by mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (NMR) revealed that catechins were a major component into condensate class of tannins. Taken as a whole, these data allow us to conclude that the AC150 of Abarema cochliacarpos has chemical compounds that present antiulcerogenic gastric and anti-inflammatory activities, which may be attributed to the tannins and (+)-catechins of this fraction. / Abstract: Abarema cochliacarpos (Gomes) Barneby & Grimes, which belongs the Mimosaceae family, is popularly known as "barbatimao" by the tradicional communities in Northeastern Brazil that use its bark in the treatment of gastric ulcer and inflammation, among other uses. In this work, the antiulcerogenic activity of its extracts and fractions was screened, using the gastric ulcer model induced by absolute ethanol in rats and selecting of the butanolic fraction of the methanolic extracts at dosis of 150 mg/kg as being in the most effective one. The action mechanisms of this fraction were studied based on the mucus gastric content and gastric secretion models. We have also decided to test it in anti-inflammatory intestinal activity model. Acute toxicity of AC150 was evaluated in Swiss mice, and no symptoms or visible toxicity signs were observed. Wistar rats were used in the others experiments. The intraduodenal pathway was utilized only in the pylorus ligature model; in the other models the oral pathway was employed. The AC 150 was able to significantly increased the mucus production, as well as to change the biochemical parameters of the acid gastric secretion, reducing the gastric volume and increasing the pH, suggesting a cytoprotection and anti-secretory activity. AC150 showed healing activity in the chronic model of gastric ulcer induced by 30 % acetic acid. This was demonstrated by the reduction of lesion area, in agreement with HE histological analysis. The induction of cellular proliferation was indicated by the PCNA results. The immunolocation of HSP-70, COX-2 and VEGF in the gastric tissue suggested that the ulcer cicatrization activity was induced by the increased of the expression of these factors, as well as the increase of the mucus production highlightened in the PAS results. In the acute ulcerative colitis model induced by intra-colonic administration of trinitrobenzensulfonic acid (TNBS) (10 mg/animal) AC 100 and 150 mg/kg were able to significantly decrease the lesion and the mieloperoxidase (MPO) levels in the colonic mucosa. However, only AC150 decreased the levels of the tumour necrosis factor-alpha (TNF-a). The western blot analysis showed that AC 150 mg/kg induced the down-regulation of both cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) expression, and the involvement of signaling also demonstrated a reduction in the JNK activation in this dosis. In the chronic colitis model (TNBS, 30 mg/animal) AC 150 showed the same inhibition profile of the inflammatory parameters; nevertheless, the increase of the antiinflammatory cytokine production IL-10 was highlightened only in this model. These data suggested a significant protective effect of the chronic administration of AC during the chronic phase of the inflammatory response associated with TNBS induced colitis. Phytochemical studies by mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (NMR) revealed that catechins were a major component into condensate class of tannins. Taken as a whole, these data allow us to conclude that the AC150 of Abarema cochliacarpos has chemical compounds that present antiulcerogenic gastric and anti-inflammatory activities, which may be attributed to the tannins and (+)-catechins of this fraction. / Doutorado / Fisiologia / Doutor em Biologia Funcional e Molecular
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.unicamp.br:REPOSIP/318118 |
| Date | 15 August 2018 |
| Creators | Silva, Maria Silene da |
| Contributors | UNIVERSIDADE ESTADUAL DE CAMPINAS, Brito, Alba Regina Monteiro Souza, 1954-, Areas, Miguel Arcanjo, Batista, Leonia Maria, Cola, Maria, Salvador, Marcos José |
| Publisher | [s.n.], Universidade Estadual de Campinas. Instituto de Biologia, Programa de Pós-Graduação em Biologia Funcional e Molecular |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | 107 p. : il., application/pdf |
| Source | reponame:Repositório Institucional da Unicamp, instname:Universidade Estadual de Campinas, instacron:UNICAMP |
| Rights | info:eu-repo/semantics/openAccess |
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