Return to search

An optimised assay for quantitative, high-throughput analysis of polysialyltransferase activity

Yes / The polysialyltransferases are biologically important glycosyltransferase enzymes responsible for the biosynthesis of
polysialic acid, a carbohydrate polymer that plays a critical role in the progression of several diseases, notably cancer.
Having improved the chemical synthesis and purification of the fluorescently-labelled DMB-DP3 acceptor, we report
optimisation and validation of a highly sensitive cell-free high-throughput HPLC-based assay for assessment of human
polysialyltransferase activity.

Identiferoai:union.ndltd.org:BRADFORD/oai:bradscholars.brad.ac.uk:10454/8803
Date07 August 2016
CreatorsElkashef, Sara M., Sutherland, Mark, Patterson, Laurence H., Loadman, Paul, Falconer, Robert A.
Source SetsBradford Scholars
LanguageEnglish
Detected LanguageEnglish
TypeArticle, Accepted manuscript
Rights© 2016 Royal Society of Chemistry. Full-text reproduced in accordance with the publisher's self-archiving policy., Unspecified

Page generated in 0.0022 seconds