Yes / The polysialyltransferases are biologically important glycosyltransferase enzymes responsible for the biosynthesis of
polysialic acid, a carbohydrate polymer that plays a critical role in the progression of several diseases, notably cancer.
Having improved the chemical synthesis and purification of the fluorescently-labelled DMB-DP3 acceptor, we report
optimisation and validation of a highly sensitive cell-free high-throughput HPLC-based assay for assessment of human
polysialyltransferase activity.
| Identifer | oai:union.ndltd.org:BRADFORD/oai:bradscholars.brad.ac.uk:10454/8803 |
| Date | 07 August 2016 |
| Creators | Elkashef, Sara M., Sutherland, Mark, Patterson, Laurence H., Loadman, Paul, Falconer, Robert A. |
| Source Sets | Bradford Scholars |
| Language | English |
| Detected Language | English |
| Type | Article, Accepted manuscript |
| Rights | © 2016 Royal Society of Chemistry. Full-text reproduced in accordance with the publisher's self-archiving policy., Unspecified |
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