<p>The potential use of enzymes in
industrial synthesis of epoxidized soybean oil has been limited through the
high cost of the enzyme catalyst, in this work we evaluate the effectiveness of chemo
enzymatic epoxidation of high oleic soybean oil (HOSBO) using lipase B from <i>Candida
antarctica </i>(CALB) on immobilization support Immobead 150 and H<sub>2</sub>O<sub>2
</sub>in a solvent-free system. Additionally, we evaluated the production decay
rates for hydrolytic activity and epoxide product formation over consecutive
batches to determine half-life of the enzyme catalyst. </p>
<p> Batch epoxidation of HOSBO using CALB on 4wt%
loading shows yields higher than 90% after 12 hrs. of reaction, and with a correlation
to the consumption of double bonds suggesting that the reaction is selective
and limiting side product reactions. Non-selective hydrolysis of oil was not
found beyond the initial hydrolysis degree of raw HOSBO. Evaluations of decay
given by epoxide product formation and released free fatty acids shows a half-life
of the enzyme catalyst on these activities is of 22 ad 25 hrs. respectively. Finally,
we evaluated the physical parameters influencing this decay, and found that H<sub>2</sub>O<sub>2</sub>
presence is the most important parameter of enzyme inactivation with no
significant effect from its slowed addition. We propose a new reactor
configuration for the analysis of the specific steps on epoxide formation
through peracid intermediates. </p>
| Identifer | oai:union.ndltd.org:purdue.edu/oai:figshare.com:article/13359929 |
| Date | 14 December 2020 |
| Creators | Elena A Robles Molina (9751112) |
| Source Sets | Purdue University |
| Detected Language | English |
| Type | Text, Thesis |
| Rights | CC BY 4.0 |
| Relation | https://figshare.com/articles/thesis/EVALUATIONS_ON_ENZYMATIC_EPOXIDATION_EFFICIENCY_AND_DECAY/13359929 |
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