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Previous issue date: 2011-02-17 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior-CAPES / The aim of this study was to observe the germination, production of glomalin and monitor
development of species of mycorrhizal fungi (AMF) of the germplasm bank of Embrapa in
root organ culture (ROC) of basil and bryophytes in vitro, analyzing their interaction with the
hosts and the influence of the culture medium enriched with humic acids on fungal growth
and bryophyte Lunularia cruciata. For this, some AMF species were selected and had their
glomerospores extracted and subjected to surface disinfection process, placed in water-agar
medium and temperature-controlled chamber to germinate. A germination test was conducted
for 15 days, and the results were analyzed by ANOVA and Tukey test applied to 5%
probability. Species with germinated glomerospores (Gigaspora margarita, Glomus
manihots, Scutellospora heterogama and Glomus proliferum) were placed in ROC of purple
basil where they had their growth observed until 100 days after inoculation. Also as part of
the characterization of AMF species it was quantified the level of glomalin in the samples of
multiplication and the results were subjected to analysis of variance and Scott-Knott test at
5% probability. In the second chapter it was investigated the effect of mycorrhizal association
in ROC of purple basil, and in the third chapter the influence of different concentrations of
humic acid and association with growth of Lunularia cruciata (area and length). The results
were submitted to ANOVA and Tukey test at 5% probability. Scutellospora heterogama was
the species with higher germination rates of glomerosporos, followed by Gigaspora
margarita. The species of Glomus sporulated after formation of symbiosis. The amount of
glomalin produced by different AMF was distinct, especially in total glomalin fraction.
Different AMF species did not show difference in efficiency to promote development of
Ocimum basilicum transformed roots. The growth of basil transformed roots in the MSR was
extended from 15 days after inoculation with mycorrhizal fungi. The usage of humic acids in
the culture medium in concentrations of 20 and 80 mg CL-1 enhanced growth of bryophyte L.
cruciata, and its association with mycorrhizal fungi, as well as promoted the highest number
of spores of Gl. proliferum. The association L. cruciata and AMF was characterized as
mutualistic, since both had advantages in growth and sporulation. Gigaspora margarita and
Glomus proliferum increased growth of Lunularia cruciata. / O objetivo do trabalho foi observar a germina??o e produ??o de glomalina e acompanhar
desenvolvimento de esp?cies de fungos micorr?zicos arbusculares (FMA) do banco de
germoplasma da Embrapa em ra?zes geneticamente modificadas de manjeric?o e em bri?fitas
in vitro. Ainda, avaliar sua intera??o com os hospedeiros e a influ?ncia de meio de cultura
enriquecido com ?cidos h?micos no crescimento do fungo e da bri?fita Lunularia cruciata.
Para isso algumas esp?cies de FMAs foram selecionadas e tiveram seus glomerosporos
extra?dos e submetidos ao processo de desinfesta??o superficial, colocados em meio Agar?gua
e c?mara com temperatura controlada para germinar. Realizou-se teste de germina??o
por 15 dias e os resultados foram submetidos a an?lise de vari?ncia e aplicado teste de Tukey
? 5% de probabilidade. Esp?cies com glomerosporos germinados (Gigaspora margarita,
Glomus manihots, Scutellospora heterogama e Glomus proliferum) foram colocadas em
ra?zes modificadas de manjeric?o roxo onde tiveram seu crescimento observado at? 100 dias
ap?s a inocula??o. Ainda como parte da caracteriza??o de esp?cies de FMAs foi realizado a
quantifica??o dos teores de glomalina nas amostras de multiplica??o sendo os resultados
submetidos a an?lise de vari?ncia e aplicado teste de Scott-Knott ? 5% de probabilidade. No
segundo cap?tulo foi verificado o efeito da associa??o FMAs em ra?zes modificadas de
manjeric?o roxo e no terceiro cap?tulo a influ?ncia da associa??o ?cido h?mico em diferentes
concentra??es, bri?fita Lunularia cruciata (?rea e comprimento) e FMAs. Os resultados
foram submetidos ? an?lise de vari?ncia e teste de Tukey a 5% de probabilidade.
Scutellospora heterogama foi a esp?cie com maiores taxas de germina??o de glomerosporos,
seguida da Gigaspora margarita. As esp?cies de Glomus esporularam logo ap?s a forma??o
da simbiose. A quantidade de glomalina produzida pelos diferentes FMAs foi distinta, em
especial na fra??o glomalina total. As diferentes esp?cies de FMAs n?o apresentaram
distin??o na efici?ncia de promover o desenvolvimento das ra?zes transformadas de Ocimum
basilicum. O crescimento de ra?zes transformadas de manjeric?o em meio MSR foi ampliado
a partir dos 15 dias ap?s a inocula??o de fungos micorr?zicos. O uso de ?cidos h?micos no
meio de cultura em concentra??es de 20 e 80 mg C.L-1 incrementou o crescimento da bri?fita
Lunularia cruciata e sua associa??o com fungos micorr?zicos arbusculares, assim como
promoveram a maior esporula??o de Gl. proliferum. A associa??o Lunularia cruciata e FMAs
foi caracterizada como mutualista j? que ambos apresentaram benef?cios em crescimento e
esporula??o. Gigaspora margarita e Glomus proliferum promoveram maior crescimento de
Lunularia cruciata.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:jspui/1218 |
| Date | 17 February 2011 |
| Creators | Nobre, Camila Pinheiro |
| Contributors | Berbara, Ricardo Luiz Louro, Saggin J?nior, Orivaldo Jos?, Goto, Bruno Tomio, Silva, Eliane Maria Ribeiro da |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Agronomia e Ci?ncia do Solo, UFRRJ, Brasil, Instituto de Agronomia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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