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Previous issue date: 2018-01-10 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / (Artigo 1 Effect of a collagen stabilizing acid conditioner on adhesion to enamel and
dentin)
The aim was to evaluate the effect of different concentrations of phosphoric acid with
grape seed extract (GSE) on the enamel acid etching, on the immediate bond strength
to enamel, and on the bond strength to dentin after 12 months storage. Three different
phosphoric acid formulations with GSE were obtained: GSE5 - 5% phosphoric acid +
20% ethanol + 2% GSE; GSE10 -10% phosphoric acid + 20% ethanol + 2% GSE;
GSE20 - 20% phosphoric acid + 20% ethanol + 2% GSE; GC (control) - 37%
phosphoric acid. The enamel and dentin surfaces of third molars, extracted for
therapeutic reasons, were etched with 37% phosphoric acid for 15 s, and with the
modified acids for 30 s. The acid was rinsed, followed by application of Adper
Scotchbond Multipurpose adhesive system, and a block of composite resin was built.
In enamel, the etching pattern was evaluated by scanning electron microscopy (SEM)
and the bond strength was evaluated by conventional tensile test. In dentin, the
microtensile bond strength (?TBS) was evaluated after 24 h and 1 year of storage.
SEM images showed that GSE5 caused a more superficial enamel etching. According
to ANOVA and Tukey?s test, enamel bond strength did not differ significantly between
GC (49.00 MPa), GSE5 (42.78 MPa), GSE10 (51.55 MPa) and GSE 20 (36.18 MPa)
(p> 0.05).According to two-way ANOVA and Tukey?s test, the interaction of factors
(treatment x storage time) was significant (p=0.0001). At the 24 h of storage, GC (28.43
MPa) obtained the highest ?TBS, and did not differ significantly from GSE20 (24.11
MPa). GC and GSE20 were significantly higher to the others (p<0.05).The GSE10
(17.53 MPa) and GSE5 (16.75 MPa) did not differ significantly from each other. At the
12 months of storage, there was no significant difference in ?TBS between GC (17.71
MPa), GSE5 (20.97 MPa), GSE10 (17.59 MPa) and GSE20 (18.52 MPa). Only GSE5
and GSE10 did not significantly decrease ?TBS after 12 months of storage. It was
concluded that GSE5 caused a more superficial enamel etching pattern in relation to
the acid concentrations at 37%, 20% and 10%. However, there was no significant
difference in the bond strength to enamel among the four concentrations. GSE5 and
GSE10 promoted stability of the bond strength to dentin after 12 months of storage.
(Artigo 2 Effect of phosphoric acid with polyphenols on dentin collagen : Atomic force
microscopy study)
Objective: The aim was of this study to evaluate the effect of different concentrations
of phosphoric acid with grape seed extract (GSE) on the structure of the collagen fibrils
of dentin. Materials and methods: Samples (0.3 mm thickness and 8 mm diameter)
of the middle third of the coronal dentin of sound human molars were obtained. Half of
the dentin of each tooth was etched with phosphoric acid (5%, 10%, or 20%) for 30 s,
and the other half was etched with its corresponding phosphoric acid with 2% GSE for
30 s. The dentin of each sample was observed using an Atomic Force Microscope
(AFM). Results: AFM images showed that phosphoric acid at concentrations of 5%,
10% and 20% demineralized the dentin and exposed the collagen fibrils and their
pattern of periodicity. Phosphoric acid at each concentration with 2% GSE changed
the collagen fibril periodicity, and the collagen fibrils formed globular structures. This
change was more significant in dentin treated with 5% phosphoric acid and less
significant with 20% phosphoric acid. Conclusions: The use of 5% phosphoric acid
with 2% GSE produced denser collagenous globular structures. This conformational
change in the collagen can make it more mechanically and enzymatically resistant. / (Artigo 1 Efeito de um condicionador ?cido estabilizador de col?geno na ades?o ao
esmalte e ? dentina)
O objetivo foi avaliar o efeito de diferentes concentra??es de ?cido fosf?rico com
extrato de semente de uva (GSE) no padr?o de condicionamento ?cido em esmalte,
na resist?ncia de uni?o imediata ao esmalte e ap?s 12 meses de armazenamento em
dentina. Tr?s diferentes formula??es de ?cido fosf?rico com GSE foram obtidas: GSE5
- 5% de ?cido fosf?rico + 20% de etanol + 2% de GSE; GSE10 -10% de ?cido fosf?rico
+ 20% de etanol + 2% de GSE; GSE20 - 20% de ?cido fosf?rico + 20% de etanol +
2% de GSE; GC (controle) - ?cido fosf?rico 37%. As superf?cies do esmalte e da
dentina de dentes terceiros molares, extra?dos por raz?es terap?uticas, foram
condicionadas com ?cido fosf?rico 37% por 15 s e com os ?cidos modificados por 30
s, seguido de lavagem e aplica??o do sistema adesivo Adper Scotchbond Multiuso
(convencional de tr?s passos) e da constru??o de um bloco em resina composta. Em
esmalte, o padr?o de condicionamento foi avaliado em microscopia eletr?nica de
varredura (MEV) e a resist?ncia de uni?o foi avaliada pelo ensaio de tra??o
convencional. Em dentina, a resist?ncia de uni?o ? microtra??o (R?T) foi avaliada
ap?s 24 h e 12 meses de armazenamento. As imagens em MEV evidenciaram que o
GSE5 causou um condicionamento mais superficial do esmalte. De acordo com
ANOVA de 2 fatores e Tukey, a resist?ncia de uni?o ao esmalte n?o diferiu
estatisticamente entre GC (49,00 MPa), GSE5 (42,78 MPa), GSE10 (51,55 MPa) e
GSE20 (36,18 MPa) (p>0,05). De acordo com ANOVA de duas vias e Tukey, a
intera??o tratamento x tempo de armazenamento foi significativa (p=0,0001). No
tempo de armazenamento de 24 h, GC (28,43 MPa) obteve a maior R?T, n?o diferindo
estatisticamente do grupo GSE20 (24,11 MPa), sendo ambos estatisticamente
superiores aos demais (p<0,05). O grupo GSE10 (17,53 MPa) e o GSE5 (16,75 MPa)
n?o diferiram estatisticamente entre si. No tempo de armazenamento de 12 meses,
n?o houve diferen?a estat?stica na R?T entre o GC (17,71 MPa), GSE5 (20,97 MPa),
GSE10 (17,59 MPa) e GSE20 (18,52 MPa). Apenas GSE5 e GSE10 n?o tiveram
redu??o significativa da R?T ap?s 12 meses de armazenamento. Concluiu-se que o
GSE5 causou um condicionamento mais superficial do esmalte em rela??o ?s
concentra??es de 37%, 20% e 10%. No entanto, n?o houve diferen?a estat?stica nos
valores de resist?ncia ? tra??o imediata ao esmalte entre as quatro concentra??es.
GSE5 e GSE10 promoveram a estabilidade da resist?ncia de uni?o ? dentina ap?s 12
meses de armazenamento.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/7918 |
| Date | 10 January 2018 |
| Creators | Paludo, Tamara |
| Contributors | Spohr, Ana Maria |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Odontologia, PUCRS, Brasil, Escola de Ci?ncias da Sa?de |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -8096554818733665164, 500, 500, 600, -2070498469879244349, 2075167498588264571 |
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