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Previous issue date: 2016-05-27 / A flora??o ? um processo vital durante o ciclo de vida das plantas e ? marcado
pela convers?o do meristema apical vegetativo em reprodutivo devido a
intera??es de fatores internos e externos ? planta. Apesar de amplo
conhecimento ter sido gerado nessa ?rea, ainda se conhece muito pouco sobre
o processo de flora??o e frutifica??o em tomateiro. Pesquisas anteriores
realizadas pelo nosso grupo identificaram o cDNA hom?logo a Prote?na
Reprimida por Auxina (ARP) em bibliotecas subtrativas reprodutivas de
tomateiro. Existem poucos dados sobre a prote?na ARP na literatura, h? relatos
de que o gene ARP est? relacionado com a matura??o do fruto em morango,
dorm?ncia da gema lateral em ervilha e matura??o do p?len em tabaco, mas a
fun??o da prote?na ARP ainda n?o est? clara. Portanto, o intuito deste trabalho
foi compreender o papel da prote?na ARP no desenvolvimento vegetativo e nos
processos de flora??o e frutifica??o por meio da perda e/ou do ganho de
fun??o deste cDNA em plantas transg?nicas de tomateiro contendo o cassete
de superexpress?o em orienta??o senso e antissenso para este cDNA. Dessa
forma, foram observadas algumas altera??es fenot?picas e estruturais nas
plantas transg?nicas (35S::SlARP antissenso e senso), como flora??o e
frutifica??o precoce verificada nas plantas 35S::SlARP antissenso nas
gera??es T1 a T4, em rela??o ?s controles (transformada com o plasm?deo
vazio e n?o transformada). Na an?lise histol?gica, notaram-se ?vulos maduros
nas flores das plantas 35S::SlARP antissenso (gera??es T2 a T4), enquanto
que as flores das controles n?o apresentaram ?vulos maduros no mesmo
per?odo de tempo. Outra modifica??o observada foi o n?mero superior de
gemas laterais desenvolvidas nas plantas 35S::SlARP antissenso nas gera??es
T3 e T4 quando comparado ?s plantas 35S::SlARP senso e ?s plantas
controles (transformada com o plasm?deo vazio e n?o transformada) . Essas
produziram ramos com folhas, flores e frutos. Assim como altera??es
estruturais nos pec?olos das plantas 35S::SlARP antissenso, incluindo uma
aparente quantidade maior de elementos de vaso (xilema e floema) do que os
pec?olos as plantas 35S::SlARP senso e mutantes relacionados ? sinaliza??o
da auxina (dgt e entire) e plantas controles. Portanto, estes dados sugerem que
a prote?na ARP possa estar envolvida na flora??o, frutifica??o e na dorm?ncia
das gemas axilares em tomateiro. / Flowering is a vital process during the plant life cycle and it is characterized by
the conversion from the vegetative apical meristem into reproductive meristem
due to internal and external factors. Despite the considerable knowledge has
been produced in this field, not much is known about the flowering and fruiting
process in tomato. Furthermore, previous research from our group has
identified a cDNA with homology to AUXIN REPRESSED PROTEIN (ARP) in
reproductive subtractive libraries from tomato. In the literature, there are few
reports where the ARP gene has been associated to fruit ripening in strawberry,
dormancy in pea and pollen maturation in tobacco plants, but it is not clear the
ARP protein function yet. Therefore, the aim of this work was to understand the
role of ARP protein in vegetative development, flowering and fruit set processes
through the loss and/or gain of function using this cDNA in transgenic tomato
plants with the overexpression cassette constructs in sense and antisense
cDNA orientation. Thus, it was observed some phenotypic and structural
modifications in transgenic plants (35S::SlARP antisense and sense) such as
early flowering and fruiting that was observed in 35S::SlARP antisense plants
from the T1 to T4 progeny when compared to controls plants (with the empty
vector and wild type plant). In the histological analysis, it was observed mature
ovule in 35S::SlARP antisense flowers (progeny T2 to T4), while for the controls
plant it wasn?t observed a mature ovule at the same period of time. The other
modification observed was a higher number of lateral buds developed in
35S::SlARP antisense plants (progeny T3 and T4) when compared to
35S::SlARP sense and controls plants. These plants produced branches with
leaves, flowers and fruits. Besides, it has been observed some structural
changes in the petioles from 35S::SlARP antisense plants, including a high
number of vessel elements (xylem and phloem) when compared to 35S::SlARP
sense, and to dgt and entire mutants (related to auxin signaling) and controls
plants (with empty vector and wild type plant). Therefore, these data suggest
that ARP protein may be involved in flowering, fruit set and dormancy of axillary
buds in tomato.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/21833 |
| Date | 27 May 2016 |
| Creators | Estevam, Renata Kaline Souza |
| Contributors | 13451112825, Uchoa, Adriana Ferreira, 58024867320, Voigt, Eduardo Luiz, 93217617991, Peres, L?zaro Eustaquio Pereira, 42819334172, Benedito, Vagner Augusto, 00000000000, Scortecci, Katia Castanho |
| Publisher | PROGRAMA DE P?S-GRADUA??O EM BIOQU?MICA, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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