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mantovani_jd_me_rcla.pdf: 608682 bytes, checksum: a54bf7e58ae584d42606324be70cd136 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / A associação entre formigas cortadeiras da tribo Attini e micro-organismos é reconhecidamente vasta. No presente trabalho verificou-se a presença de variados táxons de fungos relacionados às operárias da formiga cortadeira Atta laevigata. Operárias foram coletadas do interior do ninho e na trilha de forrageio,nas cidades de Rio Claro e Bauru, e parte dessas formigas passaram por um procedimento de limpeza para retirada de micro-organismos externos ao corpo e a outra parte não foi submetida à lavagem.Dos dois grupos o DNA foi extraído e amplificado para a região rRNA ITS de fungos. Essa região foi sequenciada e utilizada na identificação de unidades taxonômicas moleculares operacionais (MOTUs). Cinquenta táxons moleculares foram identificados, sendo vinte contaminantes eventuais, facilmente removíveis pela limpeza, e 30 simbiontes internos ou mais fortemente aderidos ao corpo das operárias. Dentre esses 30 táxons, quatro (Cladosporium cladosporioides, Epicocum sp., Hypocrea virens e Nigrospora sp.) foram identificados nas duas localidades geográficas e em grande quantidade, sendo candidatos a simbiontes mais estáveis e funcionais das formigas. Entre os demais táxons prospectados houve grande frequência de Trichosporon chiarelli e Coriolopsis rigida. Esse trabalho demonstroua ampla capacidade das formigas adquirirem e transportarem uma variada microbiota e consiste num passo inicial para elucidar o papel dos fungos associados ao corpo de A. laevigata / There is a deep and widely known association among Attini ants and microorganisms. In this paper it was investigated the presence of various taxa of fungi related to the Attini leaf-cutter antAtta laevigata. Leafcutter ants were collected from inside the nests and foraging trails in two different localities (Rio Claro and Bauru), and they were washed in order to remove external microorganisms from their bodies. Leafcutters which have not been washed also were used. Ant DNA was extracted and purifiedand the fungal rRNA ITS region was amplified. PCR products were cloned and sequenced in order to identify fungal species based on the operationalmolecular taxonomic unit (MOTU) criteria. As a result, 50 fungal species were recognized; 20 of them were identified as casual contaminants and as 30 were found as symbionts more tightly adhered to ant body. Four out of these 30 symbionts (Cladosporium cladosporioids, Epicocum sp., Hypocrea virens and Nigrospora sp.) were identified in the two geographic localities and in large quantity. Thus, these are candidates to a more stable and functional symbionts of the ants. There were large quantity of the Trichosporon chiarelli and Coriolopsis rigida among the prospected ants. This paper shows the enhanced capacity of the ants to acquiring and transporting a varied of fungi and it is in an initial point to elucidate the role of fungi associated to the body ofA. laevigata / FAPESP: 11/04053-6
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.unesp.br:11449/87709 |
| Date | 06 August 2013 |
| Creators | Mantovani, Joana Daniela [UNESP] |
| Contributors | Universidade Estadual Paulista (UNESP), Júnior, Maurício Bacci [UNESP], Rodrigues, André [UNESP] |
| Publisher | Universidade Estadual Paulista (UNESP) |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | 60 f. : il., tabs. |
| Source | Aleph, reponame:Repositório Institucional da UNESP, instname:Universidade Estadual Paulista, instacron:UNESP |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -1, -1, -1 |
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