Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2016-12-06T16:15:51Z
No. of bitstreams: 1
DIS_BRUNO_SILVEIRA_ADAMI_COMPLETO.pdf: 3612629 bytes, checksum: 63b867a36eb597783caf8d145a728bfe (MD5) / Made available in DSpace on 2016-12-06T16:15:51Z (GMT). No. of bitstreams: 1
DIS_BRUNO_SILVEIRA_ADAMI_COMPLETO.pdf: 3612629 bytes, checksum: 63b867a36eb597783caf8d145a728bfe (MD5)
Previous issue date: 2016-02-29 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The number on the citations of resveratrol has been increased over the last decade. This increase is directly related to the versatility of compound, commonly known by the benefits of wine. Contiguous to resveratrol, another growing issue in the scientific community is the microbiota and its possible modulation by compounds with prebiotic action. It is well know that resveratrol does not have a consolidated mechanism of action, and large amounts of the compound are retained in the gastrointestinal tract, we analyze the influence of resveratrol ingestion and their metabolite in populations of specific bacteria. For the induction of intestinal dysbiosis, we used experimental model of type 1 diabetes in rats. The treatment with resveratrol was performed during thirty days with daily doses of 5, 10 and 20 mg.kg-1. The quantification of the metabolite and resveratrol was accomplished through UPLC-MS/MS (Ultra-Performance Liquid Chromatography-Electrospray Tandem Mass Spectrometry). Quantification of A. muciniphila, Bacteroidetes and Prevotella was carried out by qPCR (Polymerase Chain Reaction). The study demonstrated increased population of A. muciniphila, Bacteroidetes and Prevotella in both normal and diabetic rats trated with resveratrol. This increase presented nonlinear response. The metabolite production was dose-dependent, however, a significant increase was observed in healthy animals compared to diabetic rats. We also observed significantIt is also observed correlation between different bacteria and resveratrol metabolite in healthy and diabetic animals. In conclusion, our study show that treatment with resveratrol promote changes in the microbiota. Data also reveled an increase in bacterial populations very important for intestinal health, such as: A. muciniphila, Bacteroidetes in healthy and diabetic Wistar rats in nonlinear manner. / Os estudos sobre resveratrol t?m aumentado de forma not?vel nos ?ltimos anos. Este aumento est? diretamente relacionado com a versatilidade do composto, popularmente conhecido em decorr?ncia aos benef?cios do vinho. Contiguo ao resveratrol, outro assunto crescente na comunidade cient?fica ? a microbiota e sua poss?vel modula??o atrav?s de compostos com a??o prebi?tica. Sabendo-se que o resveratrol ainda n?o tem um mecanismo de a??o consolidado e, que grande quantidade do composto fica retida no trato gastrointestinal. O presente trabalho avaliou a influ?ncia da ingest?o de resveratrol e, seu metab?lito, dihidroresveratrol, em popula??es bact?rianas especificas. Para a indu??o da disbiose intestinal, foi utilizado um modelo experimental de diabetes tipo 1 em ratos. O tratamento com resveratrol foi realizado durante trinta dias, com doses di?rias de 5, 10 e 20 mg.kg-1. A quantifica??o dos metab?litos de resveratrol foi realizada atrav?s de UPLC-MS / MS (Ultra-Performance Liquid Chromatography-lectrospray Tandem Mass Spectrometry). A quantifica??o da A. muciniphila, Bacteroidetes e Prevotella ocorreu atrav?s de qPCR (Reaction Polimerase Chain). O estudo demonstrou aumento na popula??o de A. muciniphila, Bacteroidetes e Prevotella tanto em ratos normais, quanto em animais diab?ticos trados com resveratrol. Este aumento demonstrou uma resposta n?o linear. O metab?lito apresentou uma produ??o dependente da dose, no entanto, foi observado um aumento significativo nos animais saud?veis em rela??o aos ratos diab?ticos. Observaram-se tamb?m diferentes correla??es entre as Bact?rias e o metabolito do resveratrol em animais saud?veis e diab?ticos. Em suma, o presente estudo demonstra que o tratamento com resveratrol promove altera??es na microbiota. Foi observado aumento de popula??es bacterianas importantes para a sa?de intestinal, como: A. muciniphila, Bacteroidetes, em ratos Wistar saud?veis e diab?ticos, de maneira n?o linear.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/7076 |
| Date | 29 February 2016 |
| Creators | Adami, Bruno Silveira |
| Contributors | Souto, Andr? Arigony |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Biologia Celular e Molecular, PUCRS, Brasil, Faculdade de Bioci?ncias |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 8198246930096637360, 600, 600, 600, 600, 36528317262667714, -1634559385931244697, 2075167498588264571 |
Page generated in 0.0018 seconds