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Previous issue date: 2016-02-25 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / As neoplasias de gl?ndulas salivares exibem uma ampla variedade de comportamento biol?gico e grande diversidade morfol?gica, e esta heterogeneidade inerente a este grupo de tumores suscita o interesse em pesquisar estas les?es. As c?lulas-tronco s?o a principal fonte para a gera??o e manuten??o da diversidade celular e homeostase do tecido, dist?rbios na regula??o destas c?lulas podem levar ? produ??o de c?lulas-tronco alteradas, denominadas de c?lulas-tronco tumorais, que possuem potencial proliferativo e capazes de originar e/ou manter o tumor. Pesquisas acerca das c?lulas-tronco tumorais e das prote?nas a elas associadas em algumas neoplasias orais t?m sido desenvolvidas, no entanto, o papel destas em neoplasias de gl?ndulas salivares n?o est? ainda bem estabelecido. Desta forma, o objetivo deste estudo foi identificar c?lulas do par?nquima tumoral que expressam marcadores de c?lulas-tronco tumorais, atrav?s da avalia??o da imunoexpress?o do OCT4 e CD44, em uma s?rie de casos de neoplasias de gl?ndulas salivares. A amostra foi constitu?da por 20 adenomas pleom?rficos, 20 carcinomas mucoepiderm?ides e 20 carcinomas aden?ides c?sticos localizados nas gl?ndulas salivares menores e maiores. Todos os casos estudados exibiram express?o positiva para OCT4 e CD44, sendo observado que para ambos marcadores, as neoplasias localizadas nas gl?ndulas salivares maiores exibiram maior imunomarca??o quando comparada com as les?es das gl?ndulas salivares menores apresentando diferen?a estatisticamente significativa (p=<0,001). Na amostra total e no grupo das gl?ndulas salivares menores, as neoplasias malignas exibiram maior imunorreatividade para OCT4 do que o adenoma pleom?rfico. No entanto, n?o foi encontrada diferen?as estatisticamente significativas de imunoexpress?es entre as les?es e entre suas classifica??es/grada??es histomorfol?gicas. Analisando a correla??o entre as imunoexpress?es de OCT4 e CD44 foi observada uma correla??o positiva moderada (r=0,444) com signific?ncia estat?stica entre os mesmos. A elevada express?o de OCT4 e CD44 pode indicar que estas prote?nas desempenham papel importante na identifica??o de c?lulas-tronco tumorais, permitindo uma previs?o do comportamento biol?gico das neoplasias de gl?ndula salivar, apresentando n?veis menores em tumores benignos e maiores nos tumores malignos. / Salivary gland neoplasms exhibit a wide variety of biological behavior and a high
morphological diversity raises the interest in researching these lesions. The stem cells are the
main source for the generation and maintenance of cell diversity, disorders in the regulation of
these cells can lead to the production of altered stem cells, termed cancer stem cells capable of
generate the tumor. Researches on cancer stem cells and associated proteins have been
developed in some oral cancers; however, their role in salivary gland neoplasms is not well
established. Thus, the aim of this study was to identify the tumor parenchyma cells exhibiting
stem cell characteristics, by evaluating the immunoreactivity of OCT4 and CD44, in a number
of cases of salivary gland neoplasms. The sample consisted of 20 pleomorphic adenomas, 20
mucoepidermoid carcinomas and 20 adenoid cystic carcinoma located in minor and major
salivary glands. The expression of OCT4 and CD44 was evaluated by the percentage of
positive cells (PP) and the intensity of expression (IE), it is realized the sum of the scores,
resulting in the total score immunostaining (PIT) ranging 0-7. All studied cases showed
positive expression of OCT4 and CD44 and higher values than the control groups. It was
observed that for OCT4 luminal cells and non-luminal were immunostained in the case of
pleomorphic adenomas and adenoid cystic carcinoma. Already the immunoreactivity of CD44
was particularly evident in the non-luminal cells of these lesions. In mucoepidermoid
carcinomas for both markers, there was immunoreactivity in squamous and intermediate cells
and absence of staining mucous cells. For both markers, a statistically significant higher
immunostaining was verified in neoplasms located in the major salivary glands compared
with lesions in the minor salivary (p<0.001). At the total sample and in the group of minor
salivary glands, malignant neoplasms exhibited higher immunoreactivity for OCT4 than
pleomorphic adenoma. However, there was no statistically significant difference between the
lesions and between their classifications histomorphologic. Analyzing the correlation between
OCT4 and CD44 immunoexpressions, a statistically significant moderate positive correlation
(r = 0.444) was observed. The high expression of OCT4 and CD44 may indicate that these
proteins play an important role in identifying cancer stem cells, allowing a prediction of
biological behavior of salivary gland neoplasms.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/21273 |
| Date | 25 February 2016 |
| Creators | Moura, Jamile Marinho Bezerra de Oliveira |
| Contributors | 08588724472, http://lattes.cnpq.br/6671914892609743, Queiroz, Lelia Maria Guedes, 59539224420, http://lattes.cnpq.br/3265824235655776, Oliveira, Patricia Teixeira de, 70154422487, http://lattes.cnpq.br/4750650943248760, Alves, Pollianna Muniz, 03088272446, http://lattes.cnpq.br/4860117599607892, Leit?o, ?guida Cristina Gomes Henriques, 03973092443, http://lattes.cnpq.br/2563401100488039, Souza, Lelia Batista de |
| Publisher | Universidade Federal do Rio Grande do Norte, PROGRAMA DE P?S-GRADUA??O EM PATOLOGIA ORAL, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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