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almeida_ks_dr_jabo.pdf: 820438 bytes, checksum: 3ef225bc8c0c8aa18079bcab154f5e55 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / A infecção experimental por T. vivax em ovinos foi realizada com o objetivo de avaliar os sinais clínicos, a parasitemia, as alterações hematológicas, anatomopatológicas e bioquímicas, além de realizar a biometria do parasito. Para tanto, foram utilizados oito ovinos machos, sorologicamente negativos para T. vivax, sendo quatro destinados ao grupo testemunho e quatro infectados com 105 T. vivax. As colheitas de sangue foram realizadas duas vezes antes da infecção e, posteriormente, em dias determinados, destinados aos exames laboratoriais. Exames clínicos e parasitemias foram realizados diariamente; e o peso, um dia antes da infecção, e com intervalos de 30 dias, a necropsia foi realizada aos 120 dias após infecção. Observou-se que a amostra usada era típica de T. vivax e o período prépatente de um dia, com parasitemia intermitente até o final do experimento. Os sinais clínicos encontrados nos animais infectados foram: apatia, palidez das mucosas, emaciação, aumento dos linfonodos, corrimento nasal, hipertermia, alterações no peso, diarréia e neuropatias. Ocorreu diminuição significativa na contagem global de hemácias, no teor de hemoglobina, no hematócrito e no volume corpuscular médio. A anemia, no final da observação, foi microcítica normocrômica, e leucocitose causada por linfocitose. Todos os exames bioquímicos apresentaram alterações nos animais infectados em relação ao grupo testemunho exceto a tiroxina total. No geral, observou-se: diminuição do colesterol total, colesterol ligado ao HDL, do lipídio total hepático, dos glicogênios hepático e muscular, ferro, magnésio, gamaglutamiltransferase, aspartatoaminotransferase, fosfatase alcalina, creatinaquinase, lactatodesidrogenase, triiodotironina, cortisol e nas proteínas da fase aguda, exceto a transferrina e antitripsina que estiveram altas no final do período experimental... / The objective of this research was to study an experimental infection of T. vivax in sheep, evaluating clinical signs and parasitemia as well as hematological, anatomopathological and biochemical alterations and to obtain the biometric data of the parasite. Eight male sheep were used, four in the testimony group and four infected with 105 T. vivax .Blood samples were collected two times before and posterior to receiving the infection on days designated and determined by laboratory exams. The animals were examined daily, as well as the clinical aspects and parasitemia. The animals were also weighed one day before the infection and in 30 day intervals, until they were sacrificed. The following results were obtained: the sample used was typical of T. vivax and the parasites were detected in the blood on the first day after the infection of the intermittent parasite until the end of the experiment. The clinical signs found were: apathy, paleness of the mucous, emaciation, lymph gland enlargement, runny nose, hyperthermia, weight alterations, diarrhea and neurological signs. There was a significant decrease in the global hemacia count, hemoglobin and in the hematocritic and in the average packed cell volume. The anaemia, at the end of the observations, were microcitic, normochromic and leucocitosis due to the lymphocytes. All of the biochemical exams presented alterations in the animals infected in relation to the testimony group except for the thyroxine. In general, was observed: decrease in total cholesterol, HDL, total hepatic lipid, hepatic and muscular glycogen, iron, magnesium, gamaglutamiltrasnferase, aspartate aminotransferase, alkaline phosphatase, creatine kinase, lactate desidrogenase, triiodothyronine, cortisol and proteins in the acute phase except the transferrin and antitrypsin that were high at the end of the experimental period...(Complete abstract, click electronic address below)
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.unesp.br:11449/103829 |
| Date | 03 April 2007 |
| Creators | Almeida, Katyane de Sousa [UNESP] |
| Contributors | Universidade Estadual Paulista (UNESP), Nascimento, Adjair Antonio do [UNESP] |
| Publisher | Universidade Estadual Paulista (UNESP) |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | Portuguese |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | xviii, 127 f.: il. |
| Source | Aleph, reponame:Repositório Institucional da UNESP, instname:Universidade Estadual Paulista, instacron:UNESP |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -1, -1 |
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