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Previous issue date: 2015-02-26 / O objetivo foi estabelecer protocolos para obten??o e conserva??o de espermatozoides
de cutias (Dasyprocta leporina) criadas em cativeiros no semi-?rido brasileiro, visando
sua explora??o sustent?vel. Para tanto tr?s experimentos foram realizados. No primeiro,
foi avaliada a influ?ncia da intera??o entre dois tipos de sonda (ondas senoidais e
quadr?ticas) e dois protocolos de estimula??o (cont?nuo e seriado) sobre a efici?ncia da
colheita de s?men de cutias por eletroejacula??o. A intera??o mais eficiente na
obten??o de ejaculados com espermatozoides foi a que utilizou eletrodos em aneis
associado com est?mulos em s?rie (4/7; 57%, P<0,05). No segundo, foi realizada a
compara??o da a??o crioprotetora de diferentes subst?ncias (glicerol, etilenoglicol,
dimetilsulf?xido, dimetilformamida) na criopreserva??o de espermatozoides
epididim?rios. Relativo a isso, os maiores valores de motilidade (39,5?4,6%), vigor
(2,9?0,2) e integridade de membrana (30,6?4,5%) foram obtidos nas amostras
criopreservadas com glicerol quando comparada as amostras contendo etilenoglicol e
dimetilformamida, contudo, sem diferen?a (P>0,05) quando comparada as
criopreservadas com dimetilsulfoxido. Finalmente, foi analisado o efeito dos m?todos
de obten??o de espermatozoides (eletroejacula??o vs colheita epididim?ria) sobre a
qualidade dos espermatoz?ides criopreservados. Como resultado as amostras obtidas
por lavagem epididm?ria apresentaram motilidade de 25,0?10,9% e vigor 2,4?0,8 e as
obtidas por eletroejacula??o obtiveram 31,2?14,2% de motilidade e 2,2?0,7 de vigor,
por?m sem diferen?as significativas (P>0,05), denotando a possibilidade de aplicar
diretamente, com sucesso, o protocolo de criopreserva??o de espermatozoides
epididimarios de cutias (Dasyprocta leporina) para ejaculados da mesma esp?cie. Na
presente tese, foram alcan?ados avan?os significativos na obten??o e processamento de
espermatozoides de cutias, possibilitando a forma??o de bancos de germoplasmas a
partir de amostras oriundas tanto dos epid?dimos quanto por eletroejacula??o. / The general objective of this thesis was to stablish protocols to obtain and conserve
agouti (Dasyprocta leporina) sperm bred in captivity in the Brazilian semi-arid, aiming
its sustainable production. The thesis was divided in three experiments. In the first one,
we studied the influence of the interaction between two probes (quadratics and sine
waves) and two stimulation protocols (continuous and in series) on the agouti sperm
collection by electroejaculation efficiency. The most efficient interaction on this
obtainment was the one with probes with rings associated with stimuli in series (4/7;
57%, P<0.05). In the second experiment we compared the cryoprotectant effects of
different substances (glycerol, ethyleneglycol, dimethylsulfoxide, dimethylformamide)
on epididymis sperm cryopreservation. The highest values on motility (39.5?4.6%),
vigor (2.9?0.2) and membrane integrity (30.6?4.5%) were observed on the samples
cryopreserved using glycerol when compared to those with ethyleneglycol and
dimethylformamide, but there was no difference (P>0.05) when compared to the
samples cryopreserved with dimethylsulfoxide. At last, we studied the effects of the
methods to obtain sperm (electroejaculation vs epididymal collection) on post thawing
sperm quality. The samples obtained by epididymal retrograde flushing showed values
for motility of 25.0?10.9% and vigor 2.4?0.8, and those obtained by electroejaculation
had 31.2?14.2% of motility and vigor of 2.2?0.7, however, without statistical difference
(P>0.05), which shows the possibility to successfully use the epididymal sperm
cryopreservation protocol on agouti ejaculated sperm. In conclusion, significant
advances on obtainment and processing of agouti sperm were made, allowing the
establishment of germplasm banks from sperm samples obtained from the epididymis or
by electroejaculation.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/19811 |
| Date | 26 February 2015 |
| Creators | Castelo, Thib?rio de Souza |
| Contributors | 70298254387, http://lattes.cnpq.br/1959482950237684, Pereira, Alexsandra Fernandes, 91307198368, http://lattes.cnpq.br/8114638410593492, Silva, L?cia Daniel Machado da, 65696972691, http://lattes.cnpq.br/3610262531647532, Lopes, Maria Denise, 98322290810, http://lattes.cnpq.br/6666129914663018, Oliveira, Moacir Franco de, 32594950459, http://lattes.cnpq.br/8843113233262619, Silva, Alexandre Rodrigues |
| Publisher | Universidade Federal do Rio Grande do Norte, PROGRAMA DE P?S-GRADUA??O EM BIOTECNOLOGIA, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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