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Previous issue date: 2007-03-05 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Piperine (1- piperoyl piperidine) main black pepper (Pipper nigrum Linn.) and long pepper
(Piper longum Linn.) alkaloid presents several pharmacological and biochemical effects as
enzymes inhibition from hepatic metabolism interfering on xenobiotics and pro-carcinogenic
substances biotransformation activated by P-450 cytochromo cycle. According to the
scientific bibliography reports, piperine has been able in vitro for decreasing cytotoxicity as
well as aflatoxin B1 genotoxicity on mice cells through the competition by P-450
cytochromo. Due to the importance of grains contamination by aflatoxinas, this survey had as
proposal to verify piperine ability for decreasing in vivo some damages caused by these
toxins. On first, at different piperine doses (1,12; 2,25 and 4,50 mg/Kg) Lou-M strain rats
groups were inoculated by oral administrated to evaluated this amide toxicity on animal
pattern. Parameters as: weight grain, anatomopathological and histopathological analyses
beyond changes on hematological values have been researched. Piperine doses (1,12 mg/Kg)
has showed to be appropriate as well as safe on biological assays and it has been used on rats
experimental intoxication assays by aflatoxins. Three rats groups were inoculated with
piperine (1,12 mg/Kg), aflatoxins (72 μg/ 100 g) as well as piperine + aflatoxins according to
the same parameters above described. Piperine has been able for interfering in vivo on
aflatoxins toxicity, conspicuously decreasing histopathological injuries on intoxicated animals
and significantly returning immunosupression mediated by aflatoxins. / A piperina (1-piperoil piperidina), principal amida constituinte da pimenta preta (Piper
nigrum Linn.) e da pimenta longa (P. longum Linn.), apresenta diversos efeitos
farmacol?gicos e bioqu?micos, como a inibi??o de enzimas do metabolismo hep?tico,
interferindo na biotransforma??o de xenobi?ticos e subst?ncias pr?-carcin?genas ativadas pela
via do citocromo P450. A literatura descreve a piperina como sendo capaz, in vitro, de
diminuir a citotoxidez e a genotoxidez da aflatoxina B1 em c?lulas de ratos, atrav?s da
competi??o pelo citocromo P450. Devido ? import?ncia da contamina??o de gr?os por
aflatoxinas, este trabalho teve como objetivo verificar a capacidade da piperina em diminuir,
in vivo, os danos causados por essas toxinas. Inicialmente, grupos de ratos da linhagem LOUM
foram inoculados, via oral, com diferentes doses de piperina (1,12; 2,25 e 4,50 mg/kg de
peso corporal), visando avaliar a toxidez dessa amida no modelo animal empregado.
Par?metros como: ganho de peso, an?lises anatomopatol?gica e histopatol?gica, al?m das
altera??es nos valores hematol?gicos foram investigados. A dose de 1,12 mg/kg de peso
corporal de piperina demonstrou ser segura nos ensaios biol?gicos e foi empregada nos
ensaios de intoxica??o experimental de ratos com aflatoxinas. Tr?s grupos de ratos foram
inoculados com piperina (1,12 mg/kg de peso corporal), aflatoxinas (72 μg/100g de peso
corporal) e, piperina + aflatoxinas, sendo avaliados os mesmos par?metros acima descritos. A
piperina foi capaz de interferir na toxidez das aflatoxinas, in vivo, diminuindo de forma
acentuada as les?es histopatol?gicas evidenciadas nos animais intoxicados e, revertendo de
forma significativa a imunossupress?o mediada pelas aflatoxinas.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:tede/858 |
| Date | 05 March 2007 |
| Creators | Braga, Thalita Gagini |
| Contributors | Danelli, Maria das Gracas Miranda, Direito, Gl?ria Maria |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Microbiologia Veterin?ria, UFRRJ, Brasil, Microbiologia Veterin?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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