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Previous issue date: 2014-08-22 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / O sistema RANK/RANKL/OPG contribui para a compreens?o do processo e regula??o da forma??o e reabsor??o do osso, tendo sido o maior avan?o na biologia ?ssea com rela??o a osteoclastog?nese. RANKL e OPG inibem RANK regulando a forma??o, ativa??o e sobreviv?ncia de osteoclastos na remodela??o ?ssea. O objetivo desse estudo foi investigar a express?o dos marcadores RANKL/RANK/OPG em dentes de ratos reimplantados, bem como observar a rela??o entre a express?o desses marcadores com o processo de reabsor??o dent?ria e ?ssea. Foram utilizados 30 incisivos superiores direitos de 30 ratos machos, adultos, da linhagem Wistar (Rattus norvegicus albinus). Os dentes foram avulsionados e divididos em dois grupos que permaneceram extra-alveolar em ar seco: G1 (n=15) - 5 minutos e G2 (n=15) - 60 minutos, e em seguida foram reimplantados e analisados nos intervalos de 1, 3 e 7 dias. Finalizado os per?odos experimentais, ocorreu a eutan?sia dos animais. Cortes longitudinais com 5?m de espessura foram obtidos e corados pela t?cnica H/E para a an?lise histol?gica e cortes com 3?m de espessura foram submetidos ? rea??o imuno-histoqu?mica mediante a utiliza??o de anticorpos prim?rios para ratos como OPG, RANK e RANKL. Os resultados demonstraram que o sistema RANK/RANKL/OPG participa ativamente, tanto do processo de reparo de dentes de ratos reimplantados, quanto das reabsor??es dent?rias e ?sseas; que RANKL apresentou maior imunomarca??o em ambos os grupos, participando em todas as fases da reabsor??o ?ssea e dent?ria; e o aumento da express?o de RANKL foi observada em ambos os grupos em todos os intervalos de tempo, comprovando que a resposta inflamat?ria no ligamento periodontal ocorre no in?cio do processo de reparo; e que a fraca express?o de RANK e OPG e o aumento da express?o de RANKL sugere uma diminui??o da reabsor??o ativa e aumento da reabsor??o reparada no osso e no cemento, sendo maior no osso. / The RANK / RANKL / OPG sy
stem
plays an important role in bone
formation and
resorption
. This finding
has been
regarded as
one of the m
ost important
advances in the understanding of bone biology with respect to
osteoclastogenesis. The aim of this study was to investigate the expression
of
RANKL / RANK / OPG
markers
in
reimplanted t
eeth
of rats,
and to observe the
relationship between the expression of these markers
and to
oth and bone
resorption. Thirty male Wistar
rats
(Rattus norvegicus albinos) had their maxillary
right incisors extrac
ted
,
and
were
divided into 2 groups
according to the period
that the extracted teeth were kept
in dry air
before reimplantation
: G1 (n = 15)
-
5 minutes
,
and G2 (n = 15)
-
60 minutes
. After reimplantation, teeth were
analyzed at intervals of 1, 3 and 7 da
ys. After these experimental periods, the
animals were euthanized. Longitudinal sections with 5?m thick were obtained
and stained with Hematoxylin and Eosin for histological analysis
, while
3?m thick
sections were subjected to immunohistochemical
analysis
of OPG
, RANK and
RANKL. The results showed that the RANK / RANKL / OPG system actively
participates in both the repair
process,
as well
as
tooth
and bone resorption
.
Extr
a
-
alveolar time of 60 minutes before replantation caused minor expressions
of RANKL a
nd OPG, not influencing the expression of RANK; RANKL
immunostaining showed higher in both groups when compared to other
biomarkers, participating in all phases of bone and
tooth
resorption; RANKL was
associated
to
both osteoclastogenesis
and c
ell
ular
proliferation
,
and
was
expressed in both groups.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/19980 |
| Date | 22 August 2014 |
| Creators | Maia, Concei??o Aparecida Dornelas Monteiro |
| Contributors | 41291824472, http://lattes.cnpq.br/8598456030414229, Freitas, Roseana de Almeida, 28444361453, http://lattes.cnpq.br/9512014003639405, R?go, Delane Maria, 42319501487, http://lattes.cnpq.br/5517143911175495, Nonaka, Cassiano Francisco Weege, 02781932426, http://lattes.cnpq.br/0224522010734716, Carvalho, Maria Goretti Freire de, 05601592420, http://lattes.cnpq.br/8934375314306198, Galv?o, H?bel Cavalcanti |
| Publisher | Universidade Federal do Rio Grande do Norte, PROGRAMA DE P?S-GRADUA??O EM PATOLOGIA ORAL, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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