Made available in DSpace on 2015-04-14T13:30:25Z (GMT). No. of bitstreams: 1
448387.pdf: 2406290 bytes, checksum: b9c686175b2c7a33644bea96bc032d9a (MD5)
Previous issue date: 2013-03-22 / The present study aimed at analyzing the expression of toll-like receptors (TLR) 5 and 9 and forkhead box p3 regulatory T cells transcription factor (Foxp3) in oral lesions of lichen planus and lupus erythematosus. Twenty-one biopsy specimens of lichen planus, 21 of lupus erythematosus and 21 of inflammatory fibrous hyperplasia (control group) were subjected to immunohistochemical staining with anti-TLR5, anti-TLR-9 and anti-Foxp3, whose expressions were quantified using Image Pro Plus 4.5.1 software (Media Cybernetics). Immunostaining for Foxp3 differed significantly between the three groups, where the highest levels were shown by lichen planus and the lowest ones by controls. TLR5 expression did not differ significantly between lupus erythematosus and control group, but it was significantly greater in these two groups than in lichen planus. When we considered the two types of lupus separately, there was no significant difference of TLR5 between lichen planus and systemic lupus erythematosus, but discoid lupus erythematosus as well as the control group showed significantly greater values than lichen planus. TLR9 immunostaining did not differ significantly between the groups analyzed. A weak negative correlation was observed between Foxp3 and TLR5 (r= -0.279), while there was no other correlation. When this analysis was performed within each group, no correlation was observed between the variables. In conclusion, Foxp3 expression is increased in oral lesions of lichen planus and lupus erythematosus, whereas TLR5 and TLR9 do not show increased expression in these lesions. Foxp3 and TLR5 are inversely correlated. Further investigations analyzing Treg cells and proinflammatory cytokines in these diseases are warranted. / O presente estudo teve por objetivo avaliar o padr?o de express?o dos receptores toll-like (TLR) 5 e 9 e do fator de transcri??o forkhead box p3 (Foxp3) de c?lulas T regulat?rias em les?es orais de l?quen plano e l?pus eritematoso. Vinte e um esp?cimes de bi?psias de l?quen plano, 21 de l?pus eritematoso e 21 de hiperplasia fibrosa inflamat?ria (grupo-controle) foram submetidos a processamento imunoistoqu?mico com os marcadores anti-TLR5, anti-TLR9 e anti-Foxp3, e a quantifica??o da express?o desses marcadores foi realizada por meio do software Image Pro Plus 4.5.1 (Media Cybernetics). O padr?o de express?o do Foxp3 diferiu significativamente entre os tr?s grupos, sendo os maiores n?veis observados no grupo l?quen plano, e os menores, no grupo-controle. A express?o do TLR5 n?o diferiu significativamente entre os grupos l?pus eritematoso e controle, mas foi maior nesses grupos quando comparados ao grupo l?quen plano. Ao discriminarem-se os subtipos de l?pus eritematoso sist?mico e discoide, n?o foi verificada diferen?a significativa na express?o do TLR5 entre l?quen plano e l?pus sist?mico, mas foram verificados maiores n?veis desse marcador no l?pus discoide e no grupo-controle quando comparados ao grupo l?quen plano. O padr?o de express?o do TLR9 n?o diferiu significativamente entre os grupos. Foxp3 e TLR5 apresentaram correla??o negativa fraca (r= -0.279). As demais vari?veis n?o apresentaram correla??o, mesmo quando analisadas intragrupo. Os resultados do presente estudo permitem concluir que a express?o de Foxp3 est? aumentada em les?es orais de l?quen plano e l?pus eritematoso, enquanto TLR5 e TLR9 n?o exibem maior express?o nessas les?es; Foxp3 e TLR5 est?o inversamente correlacionados. Novas investiga??es sobre c?lulas T regulat?rias, receptores toll-like e citocinas pr?-inflamat?rias fazem-se necess?rias para o melhor entendimento de doen?as como l?quen plano e l?pus eritematoso.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/1216 |
| Date | 22 March 2013 |
| Creators | Trucci, Victoria Martina |
| Contributors | Cherubini, Karen |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Odontologia, PUCRS, BR, Faculdade de Odontologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -8096554818733665164, 500, 600, 4673435736271820140 |
Page generated in 0.003 seconds