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Previous issue date: 2012-05-26 / Memories are not stored in its final form immediately after the acquisition. They undergo a long stabilization process known as consolidation. Consolidated memories can become labile again after the retrieval and susceptible to the action of amnesic agents. Thus, to persist must go through the re-stabilization process called reconsolidation. Several evidences indicate that the hippocampus plays an important role in both consolidation and reconsolidation process of different memories. In this way, we decided investigate the participation of NMDA and GABA receptors of dorsal hippocampus CA1 region on the retrieval and reconsolidation of spatial memory. We used the Morris water maze task (MWM) to demonstrate that infusion of AP5 (5 μg/μl), the selective antagonist of the NMDA receptor, into the dorsal hippocampus CA1 region of rats is able to block temporarily the retrieval of spatial memory. However, when we administered different doses and different times after the reactivation session in the absence of reinforcement is not observed any effect on spatial memory reconsolidation. While agonists of the glycine site of NMDA receptors, D-SER (10 μg/μl), improves the consolidation and the reconsolidation of spatial memory. Furthermore, we found that infusion of the agonist of GABA, muscimol (0.1 μg/μl), temporarily prevents the memory retrieval of MWM. However, when the animals received infusion of muscimol before the reactivation session and the protein synthesis inhibitor, anisomycin (160 μg/μl), immediately after this session, spatial memory remains inhibited. Thus, we have demonstrated that inhibition of NMDA receptors prevents the retrieval but not the reconsolidation of the spatial memory and the NMDA agonist is capable of improving consolidation and reconsolidation of memory, while the GABA activation temporarily prevents retrieval and the impairment of memory is maintained through inhibition of protein synthesis. / As mem?rias n?o s?o armazenadas em sua forma definitiva. Logo ap?s a aquisi??o, elas passam por um longo processo de estabiliza??o conhecido como consolida??o. Mem?rias j? consolidadas podem se tornar novamente l?beis logo ap?s a evoca??o e suscet?veis a a??o de agentes amn?sicos. Dessa forma, para persistirem devem passar pelo processo de reestabiliza??o denominado, reconsolida??o. In?meras evid?ncias indicam que o hipocampo tem um papel importante tanto na consolida??o quanto na reconsolida??o de diferentes mem?rias. Assim, decidimos investigar a participa??o dos receptores NMDA e GABA da regi?o CA1 do hipocampo dorsal na evoca??o e reconsolida??o da mem?ria espacial. Utilizando a tarefa de labirinto aqu?tico de Morris (LAM) em ratos, demonstramos que a infus?o na regi?o CA1 do hipocampo do antagonista seletivo do receptor NMDA, AP5 (5 μg/μl) ? capaz de bloquear temporariamente a evoca??o da mem?ria espacial. Por?m quando administrado diferentes doses e diferentes tempos, ap?s a sess?o de reativa??o na aus?ncia de refor?o n?o se observa efeito algum sobre a reconsolida??o da mem?ria espacial. Enquanto que o agonista do s?tio da glicina no receptor NMDA, D-SER (10 μg/μl), melhora n?o s? a consolida??o, mas tamb?m a reconsolida??o da mem?ria espacial. Al?m disso, a infus?o do agonista do receptor GABA, muscimol (0,1 μg/μl), impede temporariamente a evoca??o da mem?ria do LAM. Por?m, quando os animais recebem a infus?o de muscimol antes da sess?o de reativa??o e do inibidor da s?ntese de prote?nas, anisomicina (160 μg/μl), imediatamente ap?s esta sess?o, a mem?ria espacial permanece inibida. Assim, demonstramos que a inibi??o dos receptores NMDA impede a evoca??o mas n?o a reconsolida??o da mem?ria espacial e seu agonista ? capaz de melhorar a consolida??o e a reconsolida??o dessa mem?ria, enquanto a ativa??o do receptor GABA impede temporariamente a evoca??o e o preju?zo na mem?ria ? mantido com a inibi??o da s?ntese de prote?nas.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/1697 |
| Date | 26 May 2012 |
| Creators | Furini, Cristiane Regina Guerino |
| Contributors | Cammarota, Martin Pablo |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Medicina e Ci?ncias da Sa?de, PUCRS, BR, Faculdade de Medicina |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 7620745074616285884, 500, 600, -8624664729441623247 |
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