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Previous issue date: 2016-08-12 / A periodontite ? uma doen?a cr?nica caracterizada pela inflama??o das gengivas,
degenera??o dos ligamentos periodontais, osso alveolar e cemento. ? considerada uma das
mais importantes causa da perda dent?ria em adultos. Estudos experimentais t?m
apresentado novas alternativas farmacol?gicas para o tratamento da doen?a periodontal
com a finalidade de amenizar a inflama??o e a perda ?ssea alveolar. O objetivo desse
estudo foi avaliar os efeitos da Metformina (MET) sobre a inflama??o, o estresse oxidativo
e a perda ?ssea em um modelo de periodontite induzida por ligadura em ratos. Foram
utilizados 120 ratos, albinos, da linhagem Wistar. Os quais foram divididos aleatoriamente
em cinco grupos com 24 animais cada, com os seguintes tratamentos, por 10 dias: (NL)
aus?ncia de ligadura + salina, (L) ligadura + salina, (MET 50) ligadura + 50 mg / kg de
MET, (MET 100) ligadura + 100 mg / kg de MET, e (MET 200) ligadura + 200 mg / kg de
MET. O tecido periodontal foi analisado para determinar a perda ?ssea e caracter?sticas
histol?gicas. A imuno-histoqu?mica foi utilizada para examinar a MMP-9, a COX-2, as
vias de RANK/RANKL/OPG, SOD-1 e da GPx. A an?lise de Espectroscopia UV-VIS foi
usada para examinar os n?veis de malonalde?do (MDA), glutationa (GSH), IL-1? e TNF-?.
A rea??o da cadeia de polimerase de transcri??o reversa foi usada para quantificar a
express?o do gene de AMPK, o NF-?B p65, e HMGB1. O valor de p <0,05 indicou uma
diferen?a estat?stica significativa. O tratamento com a MET 50 mg / kg reduziu
significativamente as concentra??es de malonalde?do, IL-1? e TNF-? (p <0,05); nenhuma
das doses da MET apresentou diferen?a estatisticamente significativa (p>0,05) para o
aumento do GSH quando comparado ao grupo L; A dose de MET 50 mg/kg ainda
apresentou fraca colora??o para a COX-2, MMP-9, RANK, RANK e SOD-1; exibindo
forte colora??o para a GPx e OPG; houve aumento da express?o do AMPK e diminui??o
da express?o de NF-K? p65 e HMGB1. Os achados revelaram que a Metformina diminui a
resposta inflamat?ria, o estresse oxidativo e a perda ?ssea na periodontite induzida por
ligaduras em ratos. / Periodontitis is a chronic disease characterized by gum inflammation, degeneration
of periodontal ligaments, alveolar bone and cementum. It is considered one of the most
important cause of tooth loss in adults. Experimental studies have shown pharmacological
new alternatives for the treatment of periodontal disease in order to alleviate the
inflammation and alveolar bone loss. The aim of this study was to evaluate the effects of
Metformin (MET) on inflammation, oxidative stress and bone loss in a rat model of
ligature-induced periodontitis. Male Wistar albino rats were randomly divided into 5
groups of 24 rats each and given the following treatments for 10 days: (NL) no ligation +
saline, (L) ligation + saline, (MET 50) ligation + 50 mg/kg MET, (MET 100) ligation +
100 mg/kg MET, and (MET 200) ligation + 200 mg/kg MET. Periodontal tissue was
analysed to determine the bone loss and histopathological characteristics.
Immunohistochemical was used to examine MMP-9, COX-2, the RANK/RANKL/OPG
pathway and SOD-1 and GPx. Spectroscopic UV-VIS analysis was used to examine the
levels of Malonaldehyde, glutathione, IL-1? and TNF-?. Reverse transcription polymerase
chain reaction was used to quantify the gene expression of AMPK, NF-?? p65 and
HMGB1. A p-value of <0.05 indicated a significant difference. Treatment with 50 mg/kg
MET significantly reduced concentrations of malonaldehyde, IL-1? and TNF-? (p < 0.05);
MET any of the doses statistically significant difference (p>0,05) in GSH increased
compared to Group L. Weak staining for COX-2, MMP-9, RANK, RANKL and SOD-1;
strong staining for GPx and OPG. Increased AMPK expression and decreased expression
of NF-?B p65 and HMGB1. Metformin decreases the inflammatory response, oxidative
stress and bone loss in ligature-induced periodontitis in rats.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/22153 |
| Date | 12 August 2016 |
| Creators | Pereira, Aline de Sousa Barbosa Freitas |
| Contributors | 83806059420, http://lattes.cnpq.br/3531154240424211, Batista, L?onia Maria, 46775943415, http://lattes.cnpq.br/0601720493634706, Ara?jo J?nior, Raimundo Fernandes de, 01847881459, http://lattes.cnpq.br/1903940945895093, Ara?jo, Aurigena Antunes de |
| Publisher | PROGRAMA DE P?S-GRADUA??O EM SA?DE COLETIVA, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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