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Previous issue date: 2013-02-21 / O caf? est? entre os produtos agr?colas de maior import?ncia no com?rcio internacional e sua qualidade como produto ? um importante fator na forma??o de pre?o, al?m de ser uma das bebidas mais consumidas mundialmente e por isso desperta o interesse para os estudos dos efeitos fisiol?gicos dos seus constituintes. Diante disso, o presente trabalho teve como objetivo avaliar os constituintes a??cares, s?lidos sol?veis totais, fen?licos, flavon?ides, cafe?na e determinar a acidez total, o pH e a atividade antioxidantepor diferentes m?todos (DPPH, ABTS?+ e FRAP) no gr?o de caf? cru. Tamb?m avaliar os efeitos do tratamento sub-cr?nico com cafe?na e caf? no peso corporal e dos ?rg?os (ba?o, cora??o, f?gado, rins, supra renais e test?culos), comprimento dos ossos (f?mur e t?bia), ingest?o de alimentos (s?lidos e l?quidos) e par?metros bioqu?micos (colesterol total, glicemia e triglic?rides) em ratos machos Wistar. Foram utilizados 30 ratos: Controle (C) ? receberam ra??o e ?gua; Cafe?na (CA) ? receberam ra??o e ?gua contendo 0,1% de cafe?na; Caf? 0,1% (CC1) ? receberam ra??o e caf? contendo 0,1% de cafe?na; Caf? 0,05% (CC5) ? receberam ra??o e caf? contendo 0,05% de cafe?na; caf? 0,025% (CC25) - receberam ra??o e caf? contendo 0,025% de cafe?na. As amostras de caf? das safras de 2010 e 2011 analisadas, apresentaram diferen?as significativas para os teores de fen?licos totais, flavon?ides, cafe?na, s?lidos sol?veis, a??cares totais e n?o-redutores, al?m de acidez total e pH. A atividade antioxidante total para os m?todos utilizados (ABTS, DPPH e FRAP) e teor de a??cares redutores n?o variaram significativamente de um ano para o outro. No ensaio biol?gico os animais CC1 pesaram menos do que os C, CC5 e CC25. Em rela??o ao ba?o, os CC5 pesaram menos do que os C e CC25; os CC1 menos do que os C. As supra renais dos CC1 pesaram menos do que as dos Ca e CC25. Os CC1 apresentaram menor peso dos test?culos do que os C, CA, CC5 e CC25 e os CC25 pesaram mais do que os C. Os ratos CC1 apresentaram menor comprimento do f?mur do que os C, CC5 e CC25 e menor t?bia do que os C, CA, CC5 e CC25. Os animais CC25 apresentaram maiores taxas de glicemia do que os CC1 e CC5 e os CC1 maiores taxas de colesterol total do que os C e CC25. Os animais CC1 comeram menos do que os C durante o tratamento e beberam menos na primeira semana. Os resultados obtidos poder?o servir de aux?lio para maior conhecimento sobre a interfer?ncia dos compostos qu?micos na qualidade da bebida de caf? e compreens?o de seus efeitos metab?licos no organismo. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Qu?mica, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2012. / ABSTRACT
Coffee is among the most important agricultural products in international trade and its quality as a product is an important factor in price formation, besides being one of the most widely consumed beverages worldwide and therefore arouses interest for studies of the physiological effects of its constituents. Thus, the present study was to evaluate the constituent sugars, soluble solids, phenolics, flavonoids, caffeine and determine the total acidity, pH and activity antioxidantepor different methods (DPPH, FRAP and ABTS ? +) in raw coffee beans . The objective was also to evaluated the effects of sub-chronic treatment with coffee and caffeine on body weight and organs (spleen, heart, liver, kidneys, adrenals and testicles); bone?s length (femur and tibia); food intake (solid and liquid) and biochemical parameters (total cholesterol, glucose and triglycerides) in male Wistarrats.A total of 30 rats were used: Control (C) - received food and water; Caffeine (CA) - received food and water containing 1% caffeine; Coffee 1% (CC1) - received food and coffee containing 1% caffeine; Coffee 0.05% (CC5) - received food and coffee containing 0.05% caffeine; coffee 0.025% (CC25) - received food and coffee containing 0.025% caffeine. Samples of coffee crop in 2010 and 2011 analyzed showed significant differences in the levels of total phenolics, flavonoids, caffeine, soluble solids, total sugars and non-reducing sugars, and total acidity and pH. The total antioxidant activity for the methods (ABTS, DPPH and FRAP) and reducing sugars did not vary significantly from one year to another. In the biological tests the animals CC1 weighed less than the samples C, CC25 and CC5.Regarding the spleen, CC5 weighed less than C and CC25; the CC1 less than C.The adrenal glands of CC1 weighed less than that of the Ca and CC25.The CC1 testicles weighed less than C, CA, CC5 and CC25; and CC25 were heavier than C.The CC1 rats had lower femur length than C, CC5 and CC25; and lower tibia than the C, CA, CC5 and CC25.The CC25 animals showed higher rates of glucose than the CC1 and CC5; and CC1 higher total cholesterol than the C and CC25.CC1 animals ate less than C during the treatment and drank lessduring the first week.The results obtained may serve as an aid to improve the quality of coffee beverage and the understanding of its metabolic effects in the body.
| Identifer | oai:union.ndltd.org:IBICT/oai:acervo.ufvjm.edu.br/jspui:1/515 |
| Date | January 2012 |
| Creators | Rodrigues, Iara Ribeiro |
| Contributors | Pinto, N?sia Andrade Vilela Dessimoni, Riul, T?nia Regina, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Pinto, N?sia Andrade Vilela Dessimoni |
| Publisher | UFVJM |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFVJM, instname:Universidade Federal dos Vales do Jequitinhonha e Mucuri, instacron:UFVJM |
| Rights | A concess?o da licen?a deste item refere-se ao ? termo de autoriza??o impresso assinado pelo autor, assim como na licen?a Creative Commons, com as seguintes condi??es: Na qualidade de titular dos direitos de autor da publica??o, autorizo a Universidade Federal dos Vales do Jequitinhonha e Mucuri e o IBICT a disponibilizar por meio de seus reposit?rios, sem ressarcimento dos direitos autorais, de acordo com a Lei n? 9610/98, o texto integral da obra disponibilizada, conforme permiss?es assinaladas, para fins de leitura, impress?o e/ou download, a t?tulo de divulga??o da produ??o cient?fica brasileira, e preserva??o, a partir desta data., info:eu-repo/semantics/openAccess |
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