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valsoni_bcg_me_prud.pdf: 772648 bytes, checksum: 28f7264c9a35f8e4a86819aab281245a (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O objetivo deste trabalho foi analisar os efeitos do tabagismo passivo, associado ao exercício físico por meio da natação, nos músculos sóleo e gastrocnêmio durante a prenhez e lactação de ratas. 24 ratas foram divididas em: GF (exposto à fumaça de cigarro), GC (controle), GFN (submetido ao programa de natação e exposto à fumaça de cigarro) e GN (submetido ao programa de natação). No primeiro dia da prenhez iniciou-se o protocolo de exposição à fumaça de cigarro, constituído por 30 minutos, duas vezes ao dia, durante seis semanas. Neste mesmo período foi iniciado o programa de natação, que teve duração de 60 minutos diários até o 21o dia de amamentação. Foram obtidos os músculos sóleo e gastrocnêmio para as análises histológica, histoquímica, morfométrica e tipagem das fibras. Na histologia, os grupos GF e GFN apresentaram infiltrados inflamatórios, fibras fagocitadas e necrosadas, núcleos centralizados, splittings e enrodilhamento; no GN observou-se alterações referentes à adaptação ao exercício, infiltrados, lesão sarcolemal, fibras polimórficas, atróficas e angulares. Na análise histoquímica dos grupos GF e GFN houve ausência da atividade enzimática e agregados amorfos de formazana em posição / The objective of this study was to analyze the effects of second-hand smoking, in soleus and gastrocnemius muscles associated with physical exercise by swimming during pregnancy and lactation of rats. Twenty-four rats were divided: GF (exposed to cigarette smoke), GC (control), GFN (underwent to the swimming program and exposed to cigarette smoke) and GN (underwent to the swimming program). On the first day of pregnancy procedure of exposure to cigarette smoke began, consisting in 30 minutes twice a day for six weeks. During the same period the swimming program began, which lasted 60 minutes every day until the 21st day of lactation. Soleus and gastrocnemius muscles, were obtained for histological, histochemical, morphometric analysis and fiber profiling. In histology, the groups GF and GFN showed infiltrations, necrotic and phagocytized fibers, centralized nuclei, splittings and coiling; in GN changes were observed due to exercise adaptations, infiltrations, sarcolemal lesion, polymorphic, atrophic and angular fibers. In the histochemical analysis of the groups GF and GFN there was not enzymatic activity and amorphous formazan aggregates in subsarcolemmal positions, however in GN the same changes were found in lower frequency and intensity. In regard to the measure of the cross-section of muscle fibers there weren’t significant differences among the groups, as well as, in the frequency of types of fibers of the gastrocnemius. It is concluded that exercise intensified the morphological and histochemical changes caused by nicotine but it did not influence the measurement of the smaller diameter of muscle fibers, nor the types of fibers
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.unesp.br:11449/87315 |
| Date | 10 December 2010 |
| Creators | Valsoni, Bruna Corral Garcia [UNESP] |
| Contributors | Universidade Estadual Paulista (UNESP), Filho, José Carlos Silva Camargo [UNESP] |
| Publisher | Universidade Estadual Paulista (UNESP) |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | 155 f. : il. |
| Source | Aleph, reponame:Repositório Institucional da UNESP, instname:Universidade Estadual Paulista, instacron:UNESP |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -1, -1 |
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