A expressão de E-caderina (Ecad) pode ser regulada pré ou pós transcricionalmente por Snail e Hakai, respectivamente. Nosso objetivo foi determinar a expressão de Ecad, Snail e Hakai, em células epiteliais (CE) de tecido tumoral e peritumoral de pacientes com carcinoma ductal invasivo da mama e correlacionar sua expressão ao comprometimento linfonodal axilar (LN+). As CE de amostras de tecidos de 45 pacientes (52% LN+) foram extraídas por método imunomagnético, o RNA foi extraído por RT-PCR em tempo real e utilizou-se primers específicos para a moléculas. A expressão de Ecad, Snail e Hakai não variou entre o tecido tumoral e peritumoral e não houve correlação com comprometimento linfonodal / E-cadherin (Ecad) expression may be transcriptionally or post-transcriptionally regulated by Snail and Hakai. Our aim was to determine the expression of Ecad, Snail and Hakai, in epithelial cells (EC) obtained from tumor and its adjacent tissue from women with invasive ductal breast carcinoma (IDC) and evaluate their correlation to the axillary's lymph node (LN+) involvement. Tissue from 45 patients (52% LN+) had their EC recovered by immunomagnetic antibody process, RNA was extracted and real-time RT-PCR was performed using specific primers. Ecad, Snail and Hakai mRNA expression did not vary between tumoral and peritumoral samples and their expression was not correlated to LN involvement
| Identifer | oai:union.ndltd.org:IBICT/oai:teses.usp.br:tde-07082006-110753 |
| Date | 08 June 2006 |
| Creators | Fabiana Baroni Alves Makdissi |
| Contributors | Maria Aparecida Azevedo Koike Folgueira, Alfredo Carlos Simoes Dornellas de Barros, Maria do Socorro Maciel |
| Publisher | Universidade de São Paulo, Oncologia, USP, BR |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | Portuguese |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da USP, instname:Universidade de São Paulo, instacron:USP |
| Rights | info:eu-repo/semantics/openAccess |
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