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Previous issue date: 2009-02-18 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / This study had as a purpose determine the clinic-pathological and laboratory changes in
inoculated sheeps with the Bothrops jararaca and B. jararacussu poison, for the purpose to
provide subsidies for the establishment of the diagnosis and differential diagnosis of snake
bites. The liofilized venoms were diluted in 1 ml saline and administrated subcutaneously into
four sheep. Three of the animals died, and the one that received 0. 5mg/kg (B. jararaca)
recovered. Clinical signs were observed from 7 minutes to 1 hours after inoculation. The
evolution period varied from 7 hours and 9 minutes up to 21 hours and 59 minutes. The
symptoms, independent of the dosage, were swelling (hemorrhage/oedema) of the inoculation
site, increased bleeding time and capillary filling; tachycardia, dyspnea, hipocorated mucous
membranes and diminished reaction to external stimuli in some animals. Laboratory exam
revealed pronounced normocytic and normochromic anemia, trombocytopenia, slight
reduction of fibrogen and total plasmatic protein, diminished hematocrit in too animals,
besides pronounced increase of the creatinaquinase and lactic dehydrogenase. The main
findings at necropsy were large hemorrhagic or oedema areas in the subcutaneous tissue of
the inoculation site. Besides hemorrhages/oedema, the main histopathologic alteration was
hyalinization of muscle fibers and vessels, most severe at the inoculation site. This study it
was seen, that the swelling at the inoculation site and adjacency was due to blood, for B.
jararaca venom and oedema, for B. jararacussu venom. / Esse estudo teve como objetivo determinar as altera??es cl?nico-patol?gicas e laboratoriais em
ovinos inoculados com a pe?onha de Bothrops jararaca e B. jararacussu, no intuito de
fornecer subs?dios que possam facilitar o estabelecimento do diagn?stico e do diagn?stico
diferencial dessa condi??o. Os venenos liofilizados foram dilu?dos em 1 ml de solu??o
fisiol?gica e administrados a quatro ovinos por via subcut?nea. Tr?s ovinos foram a ?bito e
um que recebeu a dose de 0,5 mg/kg (B. jararaca), recuperou-se. Os sinais cl?nicos tiveram
in?cio entre 7 minutos e 1 horas. O per?odo de evolu??o variou de 7 horas e 9 minutos a 21
horas e 59 minutos. O quadro cl?nico, independentemente das doses, caracterizou-se por
aumento de volume (hematoma/edema) no local da inocula??o, tempo de sangramento e de
preenchimento capilar aumentados; taquicardia, dispn?ia, mucosas hipocoradas e apatia em
alguns animais. O exame laboratorial revelou acentuada anemia normoc?tica normocr?mica,
trombocitopenia, acentuada redu??o de fibrinog?nio e prote?nas plasm?ticas totais,
hemat?crito diminu?do em dois animais, al?m de acentuado aumento de creatinaquinase e
desidrogenase l?tica em todos os animais. ? necropsia, os principais achados eram extensos
hematomas/edemas no local da inocula??o e tecidos adjacentes e, em dois animais, acentuado
edema pulmonar agudo (B. jararacussu). Al?m de hemorragias/edema, a principal altera??o
histopatol?gica verificada foi necrose das fibras musculares e de vasos, no local de inocula??o
e adjac?ncias. Nos ovinos deste estudo, o aumento de volume observado no local de
inocula??o e adjac?ncias era constitu?do por sangue (B. jararaca) e edema (B. jararacussu).
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:tede/778 |
| Date | 18 February 2009 |
| Creators | Gama, Ana Paula de Arag?o |
| Contributors | Peixoto, Paulo Fernando de Vargas, Tokarnia, Carlos Maria Ant?nio Hubinger |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Curso de P?s-Gradua??o em Ci?ncias Veterin?rias, UFRRJ, Brasil, Parasitologia Veterin?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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