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Previous issue date: 2008 / Objetivo: Avaliar, do ponto de vista histológico, o efeito do transplante de tecido
adiposo sobre o útero e os ovários de camundongas obesas e anovuladoras.
Métodos: Foram utilizadas camundongas B6.V-Lepob/J com 2 e 3 meses de
idade, subdivididas em grupos experimentais: 1.Controle (B6.V-Lepob/J fêmeas,
com peso normal); 2.Obesidade (B6.V-Lepob/J fêmeas, obesas); 3.Obesidade +
Transplante de tecido adiposo de camundongas doadoras com peso normal
após 7 dias (B6.V-Lepob/J fêmeas e obesas, que receberam aproximadamente
1g de tecido adiposo de camundongas com peso normal isogênicas);
4.Obesidade + Transplante de tecido adiposo de camundongas doadoras com
peso normal após 15 dias (B6.V-Lepob/J fêmeas e obesas, que receberam
aproximadamente 1g de tecido adiposo de camundongas com peso normal
isogênicas); 5.Obesidade + Transplante de tecido adiposo de camundongas
doadoras com peso normal após 45 dias (B6.V-Lepob/J fêmeas e obesas, que
receberam aproximadamente 1g de tecido adiposo de camundongas com peso
normal isogênicas). Após 7, 15 e 45 dias do transplante, os animais foram
sacrificados. Foram verificados o peso corporal e a glicemia, além da análise
histológica dos úteros e dos ovários (H/E).
Resultados e Conclusões: O grupo das obesas (n=6) apresentou elevação no
peso corporal (52,0± 1,3*) e na glicemia (418,4±33,0*) em relação ao controle
(24,0±0,7 e 100,2±2,2, respectivamente, n=5). Os transplantes após 7 dias
(n=5) e 15 dias (n=9) foram eficazes na redução da glicemia (390,2±34,0 e
270,0±38,0*, pré e pós-Tx 7 dias; e 460,0±31,4 e 125,0±8,4*, pré e pós-Tx 15
dias, respectivamente), mas não modificaram o peso corporal. Porém, após 45 dias de transplante (n=7) houve redução da glicemia (467,0±28,0 e
209,0±18,0*, pré e pós-Tx, respectivamente), assim como tendência à
diminuição no peso corpóreo observado pré e pós-Tx (50,4±2,4 e 44,0±3,3).
Valores adotados para *p< 0,0001. A histologia revelou que, após 7 e 15 dias
de Tx, os animais apresentavam ovários semelhantes aos das obesas,
incluindo presença de inúmeros folículos, interstício abundante e ausência de
corpos lúteos. Nos úteros notou-se intensa estimulação hormonal e glandular,
mitoses, raros eosinófilos e epitélio superficial íntegro e sem renovação,
sugerindo que tempos precoces de Tx são incapazes de reverter tais
alterações. Entretanto, no grupo Tx 45 dias foram visualizados úteros
praticamente iguais aos do controle, com grande número de leucócitos e
remodelação do epitélio superficial, além de ovários com reduzida quantidade
de folículos e presença de corpos lúteos, indicando ovulação. As camundongas
B6.V-Lepob/J obesas apresentam formações polimicrocísticas e estroma
abundante, mimetizando os achados ovarianos das pacientes anovuladoras. O
Tx de tecido adiposo, a longo prazo, parece ter papel na diminuição da
obesidade, mostrando-se eficiente na reversão da hiperglicemia e das
alterações ovarianas e uterinas, presentes nas camundongas obesas,
restabelecendo a ovulação. / Objective: To assess from the histological point of view the effect of adipose
tissue transplantation onto the uterus and ovaries of obese, anovulating mice.en
Method: B6.V-Lepob/J mice were used and the following experimental groups
evaluated: 1.Control group (B6.V-Lepob/J female, average weight); 2.Obesity
(B6.V-Lepob/J female, obese); 3.Obesity plus adipose tissue transplant from
mice donors which were average weight after 7 days (B6.V-Lepob/J obese
females which received adipose tissue from thin, isogenic mice); 4.Obesity plus
adipose tissue transplant from mice donors which were average weight after 15
(B6.V-Lepob/J obese females which received adipose tissue from thin, isogenic
mice); 5.Obesity plus adipose tissue transplant from mice donors which were
average weight after 45 days (B6.V-Lepob/J obese females which received
adipose tissue from thin, isogenic mice);. Within 7, 15 and 45 days of the
transplant the animals were sacrificed. The age range was between 2 and 3
months old. In addition to the histological analyses of uteri and ovaries (H/E),
these animals’ corporal weight and glycemia were verified.
Results and Conclusion: The obese group (n=6) presented with elevation in
corporal weight (52,0 ± 1,3*)and glycemia (418,4± 33,0*) in relation to the
control group (24,0 ± 0,7 and 100,2± 2,2 respectively, n=5). The transplants
after 7 days (n=5) and 15 days (n=9) were effective in reducing glycemia (390,2
± 34,0 and 270,0 ± 38,0*, 7 days pre and post transplant; and 460,0 ± 31,4 and
125,0 ± 8,4*, 15 days pre and post transplant respectively), but not in changing
corporal weight. However, within 45 days of the transplant (n=7) there was a
reduction in glycemia (467,0 ± 28,0 and 209,0 ± 18,0*, pre and post- transplant respectively), as well as a tendency to reduce corporal weight observed pre and
post transplant (50,4 ±2,4 and 44,0 ± 3,3). Values used for *p< 0,0001.
Histology revealed that within 7 and 15 days of the transplant, the animals
presented with ovaries similar to those in the obese group, containing
countless follicles, abundant interstice and absence of corpora lutea. The uteri
revealed intense hormonal and glandular stimulation, mitosis, scarse
eosinophils and the superficial epithelium whole, without renovation, indicative
of the inability of short term transplantations to reverse such alterations. In the
45-day-group, however, the uteri looked very much the same as those of the
control group, with a great number of leucocytes and remodellation of the
superficial epithelium, as well as ovaries with a reduced amount of follicles and
presence of corpora lutea, suggesting ovulation. The obese B6.V-Lepob/J mice
present with polymicrocystic formation and abundant oestrone, mimicking the
ovarian findings of the anovulating patients. The long term adipose tissue
transplantation, on the other hand, seems to play a part in reducing obesity,
proving to be efficient in reversing hypoglycemia and the ovarian and uterine
alterations present in obese mice, re-establishing ovulation
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.unifesp.br:11600/39360 |
| Date | January 2008 |
| Creators | Pereira Júnior, Melquíades [UNIFESP] |
| Contributors | Universidade Federal de São Paulo (UNIFESP), Haidar, Mauro Abi [UNIFESP] |
| Publisher | Universidade Federal de São Paulo (UNIFESP) |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | 64 f. |
| Source | reponame:Repositório Institucional da UNIFESP, instname:Universidade Federal de São Paulo, instacron:UNIFESP |
| Rights | info:eu-repo/semantics/openAccess |
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