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Previous issue date: 2015-02-26 / Altera??es epiteliais observadas em queilites act?nicas (QA) e carcinomas
epiderm?ides de l?bio inferior (CELI) s?o causadas principalmente pela exposi??o
cr?nica aos raios ultra-violetas (UV) e s?o estudadas usando diferentes marcadores
imuno-histoqu?micos procurando avaliar o processo da carcinog?nese. O objetivo
deste estudo foi avaliar comparativamente a express?o das prote?nas Ki-67 e IMP-3
em QA e CELI a fim de contribuir com informa??es adicionais sobre a carcinog?nese
em l?bio inferior. Foram estudados 33 casos de QA e 33 casos de CELI, sendo
analisadas as caracter?sticas cl?nico-patol?gicas e a imunoexpress?o do Ki-67 e
IMP-3. A an?lise imuno-histoqu?mica do Ki-67 se deu atrav?s da determina??o do
?ndice de prolifera??o (IP) e subsequente classifica??o dos casos de acordo com os
escores : 0 (0% de c?lulas positivas), +1(?30%), +2 (>30% a ?60%) e +3 (>60%).
Para aplica??o dos testes estat?sticos os casos foram classificados em: sem
marca??o (escore 0), baixa express?o (escore +1) e alta express?o (escores +2 e
+3). Para a express?o do IMP-3, foi estabelecido o percentual de c?lulas epiteliais
imunomarcadas, sendo atribu?dos os escores: 0 (correspondeu a 0%), +1 (at? 30%
das c?lulas positivas); +2 (entre 30% a 60% de c?lulas imunomarcadas) e +3 (acima
de 60% das c?lulas positivas). Foram utilizados os testes estat?sticos Qui-quadrado
de Pearson, Mann-Whitney e Wilcoxon. O n?vel de signific?ncia adotado foi de 5%. A
maioria dos caos de QA foi do sexo masculino (78,8%), com m?dia de idade de 50
anos e dos casos de CELI tamb?m predominou o sexo masculino (69,89%) com
m?dia de 62 anos. O Ki-67 se expressou em todos os casos de QAs bem como nos
casos de CELI, predominando nas duas les?es o escore 2, correspondendo a 81,8%
dos casos nas QAs e 54,5% nos CELI. A express?o do IMP-3 nas QAs ocorreu em
72,7% dos casos, com predomin?ncia em 36,3% dos casos do escore 1. J? nos
CELI o IMP-3 se expressou em 60,6% dos casos, com predomin?ncia em 27,3% dos
casos do escore 3. Estes resultados nos permite concluir que a express?o do IMP3
e da atividade proliferativa s?o eventos precoces na carcinog?nese de l?bio inferior
independente do estado da altera??o. / Epithelial changes observed in actinic cheilitis (AC) and squamous cell carcinoma of
the lower lip (LLSCC) are mainly caused by chronic exposure to ultraviolet rays (UV)
and are studied using different immunohistochemical markers trying to evaluate the
process of carcinogenesis. The objective of this study was to comparatively evaluate
the expression of Ki-67 proteins and IMP-3 in AC and LLSCC to contribute with
additional information on carcinogenesis in lower lip. A total of 33 cases of AC and 33
cases of LLSCC were studied, analyzed the clinical and pathological features and
immunostaining of Ki-67 and IMP-3. Immunohistochemical analysis of Ki-67 was
made through the determination of the proliferation index (PI) and subsequent
classification of the cases according to the scores: 0 (0% positive cells) +1 (?30%) +
2 (> 30% and ?60%) and +3 (> 60%). For statistical tests cases were classified as
unmarked (score 0), low expression (score +1) and high expression (scores +2 and
+3). For the expression of IMP-3, the percentage of immunostained epithelial cells
was established, and assigned scores: 0 (corresponding to 0%), +1 (up to 30% of
positive cells); +2 (From 30% to 60% of immunostained cells) and +3 (over 60% of
positive cells). Statistical tests chi-square test, Mann-Whitney and Wilcoxon were
used. The significance level was 5%. Most AC chaos was male (78.8%) with mean
age of 50 years and cases of LLSCC also were male (69.89%) with an average of 62
years. The Ki-67 was expressed in all cases of AC and in cases of LLSCC,
predominantly in the two injuries the score 2, corresponding to 81.8% of cases in
ACs and 54.5% in the CELI. The expression of IMP-3 in ACs occurred in 72.7% of
cases, predominantly in 36.3% of LLSCC cases score 1. Already in the IMP-3 was
expressed in 60.6% of cases, especially in 27.3% of the score of the cases 3. These
results allow us to conclude that the expression of IMP3 and proliferative activity are
early events in carcinogenesis independently lower lip state of change.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/19911 |
| Date | 26 February 2015 |
| Creators | C?mara, Adriana Costa de Souza Martins |
| Contributors | 08588724472, http://lattes.cnpq.br/6671914892609743, Nonaka, Cassiano Francisco Weege, 02781932426, http://lattes.cnpq.br/0224522010734716, Silveira, Ericka Janine Dantas da, 02869049420, http://lattes.cnpq.br/2186658404241838, Galv?o, Hebel Cavalcanti, 41291824472, http://lattes.cnpq.br/8598456030414229, Pereira, Karuza Maria Alves, 78033977353, http://lattes.cnpq.br/3193698890688967, Souza, L?lia Batista de |
| Publisher | Universidade Federal do Rio Grande do Norte, PROGRAMA DE P?S-GRADUA??O EM PATOLOGIA ORAL, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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