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Previous issue date: 2016-02-29 / CNPq / Glomalin is regarded as a hydrophobic, heat-stable and recalcitrant glycoprotein produced by arbuscular mycorrhizal fungi (AMF). Such characteristics possibly result in high amounts of this protein in the soils, as well as a reduced rate of decomposition, and it is considered an important fraction of soil organic matter. Humic Substances (HS) have been recognized for a long time as the organic component most widely distributed on the planet, present both in terrestrial and aquatic environments. They are formed from chemical and biological degradation of residues from plants, animal and microbial activity. Although with some progress on structural aspects of glomalin and humic substances, there are few studies that describe their structural differences using a spectroscopic and chemometric approach. To search for answers for these questions, having as a reference the existing environmental and ecological relationships, this study aimed to confirm the structural relationship (differences and similarities) between the glomalin and humic substances obtained from different sources, through the isolation and purification of soil protein fractions related to glomalin (Glo), and the humic substances (HSs) fractions from different environments and composted materials; characterizing glomalin and humic substances obtained by chemical, physical and spectroscopic techniques (elemental composition, UV-vis, FTIR, 13 C CP-MAS RMN, EMS), and by using chemometric techniques (Unscrambler? X 10.3). / A glomalina ? considerada como uma glicoprote?na hidrof?bica, termoest?vel e recalcitrante produzida pelos Fungos Micorr?zicos Arbusculares (FMA). Tais caracter?sticas possivelmente implicam em altas quantidades desta prote?na nos solos bem como uma reduzida taxa de decomposi??o, sendo considerada como uma importante fra??o da mat?ria org?nica do solo. As Subst?ncias H?micas (SH) t?m sido reconhecidas durante muito tempo como o componente org?nico mais amplamente distribu?do no planeta, presentes tanto em ambientes terrestres quanto aqu?ticos. Elas s?o formadas a partir da degrada??o qu?mica e biol?gica de res?duos de plantas, animais e da atividade microbiana. Embora existam alguns avan?os sobre aspectos estruturais da glomalina e as subst?ncias h?micas, ainda s?o escassos os estudos que descrevem as suas diferen?as estruturais utilizando uma abordagem espectrosc?pica e quimiom?trica. Buscando encontrar respostas a estes questionamentos, assumindo como base de estudo a rela??o ambiental e ecol?gica existente, este estudo tem como objetivo confirmar a rela??o estrutural (diferen?as e semelhan?as) existente entre a glomalina e as substancias h?micas de diferentes origens, atrav?s do isolamento e purifica??o das fra??es da prote?na do solo relacionada ? glomalina (Glo) e as fra??es de subst?ncias h?micas (SHs) de diferentes ambientes e materiais compostados; caracterizando a glomalina e as subst?ncias h?micas obtidas atrav?s de t?cnicas qu?micas, f?sicas e espectrosc?picas (composi??o elementar, UV-vis, FTIR, CP-MAS 13C RMN, MEV) e atrav?s da utiliza??o de t?cnicas quimiom?tricas (Unscrambler? X 10.3).
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:jspui/1907 |
| Date | 29 February 2016 |
| Creators | SOUZA, Luiz Gilberto Ambr?sio de |
| Contributors | Berbara, Ricardo Luiz Louro, Garc?a, Andr?s Calder?n, Berbara, Ricardo Luiz Louro, Ara?jo, Ednaldo da Silva, Pereira, Marcos Gervasio |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Agronomia e Ci?ncia do Solo, UFRRJ, Brasil, Instituto de Agronomia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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