Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2016-01-05T13:42:22Z No. of bitstreams: 2 uidemar_morais_barral.pdf: 1274629 bytes, checksum: d89bbca6115310a0b5ad8323167ba551 (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2016-01-05T18:01:03Z (GMT) No. of bitstreams: 2 uidemar_morais_barral.pdf: 1274629 bytes, checksum: d89bbca6115310a0b5ad8323167ba551 (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) / Made available in DSpace on 2016-01-05T18:01:03Z (GMT). No. of bitstreams: 2 uidemar_morais_barral.pdf: 1274629 bytes, checksum: d89bbca6115310a0b5ad8323167ba551 (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) Previous issue date: 2015 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (Capes) / A mat?ria org?nica (MOS) do solo consiste de uma mistura de compostos em v?rios est?gios de decomposi??o, resultante da degrada??o biol?gica de res?duos de plantas e animais, e da atividade de microrganismos, denominados subst?ncias h?micas (SHs). Essas subst?ncias fracionadas em ?cidos f?lvicos (AF), ?cidos h?micos (AH) e humina (H), de acordo com sua solubilidade em meio ?cido ou b?sico. Os AH t?m sido usados como fertilizantes aplicados diretamente no solo ou via foliar, principalmente por influenciarem o metabolismo das plantas. Objetivou-se com este trabalho avaliar em feijoeiro (Phaseolus vulgaris L.), a influ?ncia de fontes e doses diferentes de AH, na produ??o, crescimento radicular e absor??o de nutrientes e biodisponibilidade de nutrientes no solo. O experimento foi conduzido utilizando o delineamento em blocos casualizados com 15 tratamentos, sendo estes as fontes de AH (turfa - 1, composto - 2 e produto comercial - 3) e as doses de AH (0, 2, 8, 16 e 32 kg ha-1), com quatro repeti??es. Foram avaliadas: altura das plantas (cm), di?metro do caule (cm), teor de clorofila (?g cm-2) e teor de macro e micronutrientes nas folhas no florescimento do feijoeiro e ao fim do experimento, peso de sementes (g), massa seca da parte a?rea e de ra?zes (g), n?mero de vagem por planta e de sementes por vagem e no solo: pH em ?gua e teores de mat?ria org?nica, P, K, Ca, Mg, Al e H+Al. A aplica??o de AH reduziu o crescimento e produ??o de gr?o, com aumento das doses em cada fonte, seguindo a ordem fonte 3 > fonte 1 > fonte 2. Os teores de P, K, Cu, Fe, Mn e Zn nas folhas foram influenciados pelas doses de todas as fontes de AH. Houve redu??o para maioria dos nutrientes quantificados nas folhas com aumento das doses de AH. Os teores de P e K se elevaram no solo, com a aplica??o de AH. Para condi??es testadas, n?o se recomenda utiliza??o de AH para cultura do feijoeiro. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2015. / ABSTRACT The soil organic matter (SOM) consists of a mixture of compounds in many decomposition stages, the result of biological degradation of residues of plants and animals, and microorganisms activity, called humic substances (HS). These substances are fractionated into fulvic acids (FA), humic acids (HA) and humin (H), according to their solubility in acidic or basic medium. HA have been used as fertilizers directly applied to the soil or via foliar, mainly because they influence plants metabolism. The main goal of this study was evaluating, in bean plant (Phaseolus vulgaris L.), the influence of sources and different doses of HA in production, root growth, nutrients absorption and bioavailability of nutrients in the soil. The experiment was conducted using randomized block design with 15 treatments, which are the sources of HA (peat ? 1, compound ? 2 and commercial product ? 3) and doses of HA (0, 2, 8, 16 e 32 kg ha-1), with four repetitions. Evaluations were made on: plants height (cm), stem diameter (cm), chlorophyll content (?g cm-2) and content of macro and micronutrients in the leaves in the flowering stages and in the end of the experiment, seeds weight (g), dry matter of the aerial part and roots (g), number of pods per plant and seeds per pod, and in the soil: pH in water and contents of organic matter, P, K, Ca, Mg, Al and H+Al. Application of HA reduced bean growth and production, with increase of doses in each source, following order: source 3 > source 1 > source 2. Contents of P, K, Cu, Fe, Mn and Zn in the leaves were influenced by the doses of all HA sources. Most of the nutrients quantified in the leaves were reduced with increasing HA doses. Contents of P and K were increased in the soil with application of HA. For tested conditions, the use of HA for bean culture is not recommended.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-07-25T17:57:36Z No. of bitstreams: 1 2016 - Luiz Gilberto Ambr?sio de Souza.pdf: 3023562 bytes, checksum: b3607c9323156b54bb63bceacd5de74e (MD5) / Made available in DSpace on 2017-07-25T17:57:36Z (GMT). No. of bitstreams: 1 2016 - Luiz Gilberto Ambr?sio de Souza.pdf: 3023562 bytes, checksum: b3607c9323156b54bb63bceacd5de74e (MD5) Previous issue date: 2016-02-29 / CNPq / Glomalin is regarded as a hydrophobic, heat-stable and recalcitrant glycoprotein produced by arbuscular mycorrhizal fungi (AMF). Such characteristics possibly result in high amounts of this protein in the soils, as well as a reduced rate of decomposition, and it is considered an important fraction of soil organic matter. Humic Substances (HS) have been recognized for a long time as the organic component most widely distributed on the planet, present both in terrestrial and aquatic environments. They are formed from chemical and biological degradation of residues from plants, animal and microbial activity. Although with some progress on structural aspects of glomalin and humic substances, there are few studies that describe their structural differences using a spectroscopic and chemometric approach. To search for answers for these questions, having as a reference the existing environmental and ecological relationships, this study aimed to confirm the structural relationship (differences and similarities) between the glomalin and humic substances obtained from different sources, through the isolation and purification of soil protein fractions related to glomalin (Glo), and the humic substances (HSs) fractions from different environments and composted materials; characterizing glomalin and humic substances obtained by chemical, physical and spectroscopic techniques (elemental composition, UV-vis, FTIR, 13 C CP-MAS RMN, EMS), and by using chemometric techniques (Unscrambler? X 10.3). / A glomalina ? considerada como uma glicoprote?na hidrof?bica, termoest?vel e recalcitrante produzida pelos Fungos Micorr?zicos Arbusculares (FMA). Tais caracter?sticas possivelmente implicam em altas quantidades desta prote?na nos solos bem como uma reduzida taxa de decomposi??o, sendo considerada como uma importante fra??o da mat?ria org?nica do solo. As Subst?ncias H?micas (SH) t?m sido reconhecidas durante muito tempo como o componente org?nico mais amplamente distribu?do no planeta, presentes tanto em ambientes terrestres quanto aqu?ticos. Elas s?o formadas a partir da degrada??o qu?mica e biol?gica de res?duos de plantas, animais e da atividade microbiana. Embora existam alguns avan?os sobre aspectos estruturais da glomalina e as subst?ncias h?micas, ainda s?o escassos os estudos que descrevem as suas diferen?as estruturais utilizando uma abordagem espectrosc?pica e quimiom?trica. Buscando encontrar respostas a estes questionamentos, assumindo como base de estudo a rela??o ambiental e ecol?gica existente, este estudo tem como objetivo confirmar a rela??o estrutural (diferen?as e semelhan?as) existente entre a glomalina e as substancias h?micas de diferentes origens, atrav?s do isolamento e purifica??o das fra??es da prote?na do solo relacionada ? glomalina (Glo) e as fra??es de subst?ncias h?micas (SHs) de diferentes ambientes e materiais compostados; caracterizando a glomalina e as subst?ncias h?micas obtidas atrav?s de t?cnicas qu?micas, f?sicas e espectrosc?picas (composi??o elementar, UV-vis, FTIR, CP-MAS 13C RMN, MEV) e atrav?s da utiliza??o de t?cnicas quimiom?tricas (Unscrambler? X 10.3).

Submitted by Leticia Schettini (leticia@ufrrj.br) on 2016-09-21T15:12:59Z No. of bitstreams: 1 2011 - Camila Pinheiro Nobre.pdf: 2714138 bytes, checksum: bc436d879ddfded703d4d5fea9f9943c (MD5) / Made available in DSpace on 2016-09-21T15:12:59Z (GMT). No. of bitstreams: 1 2011 - Camila Pinheiro Nobre.pdf: 2714138 bytes, checksum: bc436d879ddfded703d4d5fea9f9943c (MD5) Previous issue date: 2011-02-17 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior-CAPES / The aim of this study was to observe the germination, production of glomalin and monitor development of species of mycorrhizal fungi (AMF) of the germplasm bank of Embrapa in root organ culture (ROC) of basil and bryophytes in vitro, analyzing their interaction with the hosts and the influence of the culture medium enriched with humic acids on fungal growth and bryophyte Lunularia cruciata. For this, some AMF species were selected and had their glomerospores extracted and subjected to surface disinfection process, placed in water-agar medium and temperature-controlled chamber to germinate. A germination test was conducted for 15 days, and the results were analyzed by ANOVA and Tukey test applied to 5% probability. Species with germinated glomerospores (Gigaspora margarita, Glomus manihots, Scutellospora heterogama and Glomus proliferum) were placed in ROC of purple basil where they had their growth observed until 100 days after inoculation. Also as part of the characterization of AMF species it was quantified the level of glomalin in the samples of multiplication and the results were subjected to analysis of variance and Scott-Knott test at 5% probability. In the second chapter it was investigated the effect of mycorrhizal association in ROC of purple basil, and in the third chapter the influence of different concentrations of humic acid and association with growth of Lunularia cruciata (area and length). The results were submitted to ANOVA and Tukey test at 5% probability. Scutellospora heterogama was the species with higher germination rates of glomerosporos, followed by Gigaspora margarita. The species of Glomus sporulated after formation of symbiosis. The amount of glomalin produced by different AMF was distinct, especially in total glomalin fraction. Different AMF species did not show difference in efficiency to promote development of Ocimum basilicum transformed roots. The growth of basil transformed roots in the MSR was extended from 15 days after inoculation with mycorrhizal fungi. The usage of humic acids in the culture medium in concentrations of 20 and 80 mg CL-1 enhanced growth of bryophyte L. cruciata, and its association with mycorrhizal fungi, as well as promoted the highest number of spores of Gl. proliferum. The association L. cruciata and AMF was characterized as mutualistic, since both had advantages in growth and sporulation. Gigaspora margarita and Glomus proliferum increased growth of Lunularia cruciata. / O objetivo do trabalho foi observar a germina??o e produ??o de glomalina e acompanhar desenvolvimento de esp?cies de fungos micorr?zicos arbusculares (FMA) do banco de germoplasma da Embrapa em ra?zes geneticamente modificadas de manjeric?o e em bri?fitas in vitro. Ainda, avaliar sua intera??o com os hospedeiros e a influ?ncia de meio de cultura enriquecido com ?cidos h?micos no crescimento do fungo e da bri?fita Lunularia cruciata. Para isso algumas esp?cies de FMAs foram selecionadas e tiveram seus glomerosporos extra?dos e submetidos ao processo de desinfesta??o superficial, colocados em meio Agar?gua e c?mara com temperatura controlada para germinar. Realizou-se teste de germina??o por 15 dias e os resultados foram submetidos a an?lise de vari?ncia e aplicado teste de Tukey ? 5% de probabilidade. Esp?cies com glomerosporos germinados (Gigaspora margarita, Glomus manihots, Scutellospora heterogama e Glomus proliferum) foram colocadas em ra?zes modificadas de manjeric?o roxo onde tiveram seu crescimento observado at? 100 dias ap?s a inocula??o. Ainda como parte da caracteriza??o de esp?cies de FMAs foi realizado a quantifica??o dos teores de glomalina nas amostras de multiplica??o sendo os resultados submetidos a an?lise de vari?ncia e aplicado teste de Scott-Knott ? 5% de probabilidade. No segundo cap?tulo foi verificado o efeito da associa??o FMAs em ra?zes modificadas de manjeric?o roxo e no terceiro cap?tulo a influ?ncia da associa??o ?cido h?mico em diferentes concentra??es, bri?fita Lunularia cruciata (?rea e comprimento) e FMAs. Os resultados foram submetidos ? an?lise de vari?ncia e teste de Tukey a 5% de probabilidade. Scutellospora heterogama foi a esp?cie com maiores taxas de germina??o de glomerosporos, seguida da Gigaspora margarita. As esp?cies de Glomus esporularam logo ap?s a forma??o da simbiose. A quantidade de glomalina produzida pelos diferentes FMAs foi distinta, em especial na fra??o glomalina total. As diferentes esp?cies de FMAs n?o apresentaram distin??o na efici?ncia de promover o desenvolvimento das ra?zes transformadas de Ocimum basilicum. O crescimento de ra?zes transformadas de manjeric?o em meio MSR foi ampliado a partir dos 15 dias ap?s a inocula??o de fungos micorr?zicos. O uso de ?cidos h?micos no meio de cultura em concentra??es de 20 e 80 mg C.L-1 incrementou o crescimento da bri?fita Lunularia cruciata e sua associa??o com fungos micorr?zicos arbusculares, assim como promoveram a maior esporula??o de Gl. proliferum. A associa??o Lunularia cruciata e FMAs foi caracterizada como mutualista j? que ambos apresentaram benef?cios em crescimento e esporula??o. Gigaspora margarita e Glomus proliferum promoveram maior crescimento de Lunularia cruciata.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-02-07T18:57:15Z No. of bitstreams: 1 2016 - Shirlei Almeida Assun??o.pdf: 1407109 bytes, checksum: 346922df83ba0b7c92b59002d73737df (MD5) / Made available in DSpace on 2017-02-07T18:57:15Z (GMT). No. of bitstreams: 1 2016 - Shirlei Almeida Assun??o.pdf: 1407109 bytes, checksum: 346922df83ba0b7c92b59002d73737df (MD5) Previous issue date: 2016-02-22 / CAPES / Faperj / Brazil is the biggest sugarcane producer of the world with cultivated area superior to nine millions hectares. To reach economically viable productivity levels it is necessary to make use of high amounts of N fertilizers. These fertilizers are high cost and they also can contribute to emission of greenhouse gases and pollution of watercourses, as well as, to influence directly on the decomposition of the soil organic matter (SOM). In this context to understand how interactions between nitrogen and SOM occurs in sugarcane fields is important from both economic and environmental points of view. Therefore, the objective of this study was to evaluate quantity and quality of soil carbon when different doses of nitrogen are added in the form of ammonium sulphate in a sugarcane raw agro-ecosystem in an ultisol from Coastal Plains. The experiment was conduced in sugarcane fields renovated in 2009 in area of the company LASA alcohol distillery, in Linhares - ES, in an Ultisol with sandy to medium texture, from Coastal Plain environment. The variety of sugarcane (Saccharum spp.) used was RB918639. There were evaluated five treatments of N ammonium sulphate doses: 0 (control), 80, 100, 120 and 160 kg of N per ha-1 with four repetitions, established under experimental design of randomized blocks. Each experimental unit (plot) was 70 m? (5 lines with 10 m, spaced by 1.4 m between lines). Samples of plant and soil were collected in September 2013 and 2014, corresponding to 4th and 5th harvest. The results indicated that among the N doses evaluated, the doses of 100 and 120 kg were the most efficient on accumulating carbon on the humic fractions, particle size, densimetric and oxidizable carbon fractions. The dose of 160 kg was the less effective to carbon accumulation on the humic fractions, particle size, densimetric and oxidizable carbon fractions. Total nitrogen, natural abundance of 13C and 15N, stem and straw production were not influenced by N rates, with no differences on any of the seasons. About the elemental composition of humic acids (HA), the quantities of chemicals were very similar between doses and coherent with results in the literature for the HA. In the E4/E6 ratio, the dose of 160 kg of N was the one with the lowest E4/E6 ratio. Regarding the infrared with Fourier transformation it was observed the predominance of aliphatic groups, and the analysis of major components enabled the clear separation of the effects from different N doses. In the 13CNMR prevailed larger amounts of aliphatic carbons, it was confirmed with the aliphaticity index of the samples, the results were above 80% for all samples. It was also verified through the 13 CNMR that the control area and the dose of 160 kg had the lowest aromaticity rates. Therefore, it was concluded that different fractionations of organic matter along with spectroscopic techniques were effective on evaluating the influence of different nitrogen doses to the decomposition of SOM. / O Brasil ? o maior produtor mundial de cana-de-a??car, com ?rea plantada que ultrapassa os nove milhoes de hectares. Para obter produtividade economicamente vi?vel da cultura, s?o utilizadas elevadas doses de fertilizantes nitrogenados. Tais fertilizantes possuem elevados custos economicos, podem contribuir para emiss?o de gases do efeito estufa e para polui??o de cursos de ?gua, al?m de infuenciarem de forma direta na decomposi??o da mat?ria org?nica do solo (MOS). Neste contexto, entender como ocorre a intera??o entre o nitrog?nio e a MOS no cultivo da cana ? importante tanto do vista economico quanto ambiental. Assim sendo o objetivo desse estudo foi avaliar a quantidade e qualidade do carbono do solo em fun??o de diferentes doses de nitrog?nio adicionado na forma de sulfato de am?nio no agroecossistema cana-de-a??car crua em Argissolo Amarelo de Tabuleiro Costeiro. O experimento foi instalado em canavial renovado em 2009, em ?rea cedida pela Usina LASA, no munic?pio de Linhares ? ES, em Argissolo Amarelo de textura arenosa/m?dia, no ambiente de Tabuleiros Costeiros. A variedade da cana-de-a??car (Saccharum spp.) utilizada foi a RB918639. Foram avaliados cinco tratamentos/doses de N-sulfato de am?nio quais sejam: 0 (testemunha), 80, 100, 120 e 160 kg ha-1 de N com quatro repeti??es, estabelecidos segundo delineamento experimental de blocos ao acaso. Cada unidade experimental (parcela) tinha 70 m? (5 linhas com 10 m, espa?adas 1,4 m). As amostras de planta e terra foram coletadas no m?s de setembro de 2014, correspondendo ? 4? soca. Os resultados indicaram que as doses de 100 e 120 kg, foram as mais eficientes para acumular o carbono nas fra??es h?micas, granulom?tricas, densim?tricas e nas fra??es do carbono oxid?vel. A dose de 160 kg foi a menos eficiente para acumular o carbono nas fra??es h?micas, granulom?tricas, densim?tricas e nas fra??es do carbono oxid?vel. O nitrog?nio total, a abund?ncia natural de 13C e 15N e a produtividade de colmo e palhada n?o apresentaram diferen?as significativas. Na composi??o elementar dos ?cidos h?micos (AH), as quantidades dos elementos qu?micos foram bem semelhantes entre as doses e coerentes com resultados na literatura para os AH. Para o coeficiente E4/E6 a dose de 160 kg de N foi a que apresentou o menor coeficiente E4/E6. Em rela??o ao infravermelho com transforma??o de Fourier verificou-se o predom?nio de grupamentos alif?ticos, e a analise de componentes principais possibilitou a separa??o clara do efeito das diferentes doses de N. No RMN C prevaleceram maiores quantidades de carbonos alif?ticos, isto foi confirmado com o ?ndice de alif?ticidade das amostras que foram superiores a 80 % para todas as amostras. Verificou-se tamb?m atrav?s do RMN 13C que a testemunha e a dose de 160 kg foram as que apresentaram menores ?ndices de aromaticidade. Portanto, conclui-se que os diferentes fracionamentos bem com as t?cnicas espectrosc?picas foram eficientes para avaliar a influencia das diferentes doses de N na decomposi??o da MOS.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T13:30:33Z No. of bitstreams: 5 3.pdf: 1293801 bytes, checksum: 8df08be550789bd3f1c5c25b92deba50 (MD5) license_url: 49 bytes, checksum: fd26723f8d7edacdb29e3f03465c3b03 (MD5) license_text: 20626 bytes, checksum: 7851d446050a985bd0603b7c4cee472d (MD5) license_rdf: 19710 bytes, checksum: aa65da15f424ff71c620a992a187295c (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T18:19:32Z (GMT) No. of bitstreams: 5 3.pdf: 1293801 bytes, checksum: 8df08be550789bd3f1c5c25b92deba50 (MD5) license_url: 49 bytes, checksum: fd26723f8d7edacdb29e3f03465c3b03 (MD5) license_text: 20626 bytes, checksum: 7851d446050a985bd0603b7c4cee472d (MD5) license_rdf: 19710 bytes, checksum: aa65da15f424ff71c620a992a187295c (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-27T18:19:32Z (GMT). No. of bitstreams: 5 3.pdf: 1293801 bytes, checksum: 8df08be550789bd3f1c5c25b92deba50 (MD5) license_url: 49 bytes, checksum: fd26723f8d7edacdb29e3f03465c3b03 (MD5) license_text: 20626 bytes, checksum: 7851d446050a985bd0603b7c4cee472d (MD5) license_rdf: 19710 bytes, checksum: aa65da15f424ff71c620a992a187295c (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2009 / A turfeira ? formada pelo ac?mulo em sucess?o de restos vegetais, em locais que apresentam condi??es que inibem a atividade de microrganismos decompositores, como excesso de umidade, baixo pH, escassez de oxig?nio e temperaturas amenas. Em Diamantina, esse pedoambiente ? encontrado na ?rea de Prote??o Ambiental - APA Pau-de-Fruta, situada a 6 km da sede do munic?pio, a uma altitude m?dia de 1366 m. A APA est? inserida na Serra do Espinha?o Meridional, sua litologia ? predominantemente quartz?tica e a vegeta??o ? t?pica de campo rupestre, com pequenas ilhas de cerrado denominadas cap?es, que se adaptaram ao ambiente hidrom?rfico. O ambiente ? oligotr?fico e apresenta elevados teores de Al3+ e valores de satura??o por alum?nio. As turfeiras formadas nessa ?rea apresentam verticalmente uma estrutura bem definida, sendo que as camadas mais superficiais foram classificadas, de acordo com seu est?gio de decomposi??o, como f?bricas, as intermedi?rias como h?micas e as camadas mais profundas, como s?pricas. A turfeira, por ser um ambiente de ac?mulo de mat?ria org?nica em condi??es de baixa atividade de O2, favorece a forma??o e a manuten??o de subst?ncias h?micas, sobretudo as fra??es menos sol?veis, de forma que o teor de humina ? maior que os teores de ?cidos h?micos que, por sua vez, s?o maiores que o teor de ?cidos f?lvicos. A turfeira, devido ao seu comportamento tipo esponja, apresenta grande import?ncia na din?mica da ?gua nessa regi?o, de forma que, nos per?odos chuvosos, ela armazena ?gua em seus poros e a libera de forma gradativa com o passar do tempo. A turfeira da APA Pau-de-Fruta ocupa 81,75 ha, armazena cerca de 629.782 m3 de ?gua e estoca em torno de 33.129 toneladas de carbono. Dessa forma, a turfeira da APA Pau-de-Fruta representa um consider?vel reservat?rio natural de ?gua, bem como o importante ambiente de sequestro de carbono e ? fundamental para o abastecimento de ?gua da cidade de Diamantina. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2009. / ABSTRACT The Peat Bog is formed by the accumulation in succession of crop residues in places with conditions that inhibit the activity of microbial decomposers, such as excessive moisture, low pH, lack of oxygen and mild temperatures. In Diamantina, this is found in the Environmental Protection Area - EPA Pau-de-Fruta, situated 6 km from the headquarters of the city, at an average altitude of 1,366 m. The EPA is inserted in the Serra do Espinha?o Meridional, its lithology is predominantly quartz and its vegetation is typical of rocky field, with small islands of ?cerrado? (vegetation of the Brazilian interior) called ?cap?es?, which are adapted to the hydromorphic environment. The environment is oligotrophic and offers high levels of Al3+ and the values of saturation by aluminum. The bogs formed in this area have a vertically well-defined structure and, according to their stage of decomposition, the more superficial layers were classified as ?f?bricas?, the intermediate layers as ?h?micas?, and the deeper layers as ?s?pricas?. The bog, being an environment of accumulation of organic material under conditions of low activity of O2, promotes the formation and maintenance of wet substances, especially the less soluble fractions, so that the content of humin is greater than the levels of humic acids which in turn are greater than the content of fulvic acids. The bog, because of its behavior type sponge, has great importance in the dynamics of water in this region, so that in rainy periods it stores water in its pores and releases it gradually over the time. The bog of the EAP Pau-de-Fruta occupies 81.75 ha, stores about 629,782 m3 of water and stocks around 33,129 tonnes of carbon. Thus, the bog of of the EAP Pau-de-Fruta is a considerable natural reservoir of water, and an important environment of carbon sequestration and it is essential for the supply of water for the city of Diamantina.