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Previous issue date: 2015-03-19 / As doen?as periodontais possuem alta preval?ncia na popula??o mundial e
manifestam-se, principalmente, em duas entidades distintas, a saber: a gengivite induzida pela
placa bacteriana e a periodontite. A periodontite ? uma doen?a inflamat?ria cr?nica
caracterizada pela destrui??o em v?rios n?veis do osso alveolar, fibras col?genas e do
cemento, ? considerada importante causa de perda dent?ria em adultos. Estudos
experimentais recentes demonstram o efeito anti-inflamat?rio e antirreabsortivo dos f?rmacos
anti-hipertensivos da classe dos bloqueadores dos receptores de angiotensina II sobre a
doen?a periodontal. O objetivo desse estudo foi avaliar os efeitos da azilsartana (AZT) um
potente inibidor dos receptores de angiotensina II que apresenta efeitos adversos m?nimos,
sobre a perda ?ssea alveolar, inflama??o, express?o das metaloproteinases da matriz (MMP),
ligante do receptor do fator nuclear kapa B (RANKL), receptor ativador do fator nuclear kapa
B (RANK), osteoprotegerina (OPG), ciclooxigenase-2 (COX-2), Interleucina 10, Interleucina
1?, TNF-?, glutationa (GSH) e catepsina K no modelo de doen?a periodontal induzida por
ligadura em ratos. Foram utilizados 100 ratos wistar sendo divididos randomicamente em 5
grupos com 20 ratos cada: (1) n?o foi induzida a doen?a periodontal, ?gua destilada, (2)
indu??o da doen?a periodontal, ?gua destilada; (3) indu??o da doen?a periodontal, AZT 1
mg/kg; (4) indu??o da doen?a periodontal, AZT 5 mg/kg; e (5) indu??o da doen?a
periodontal, AZT 10 mg/kg. Todos os grupos foram tratados com ?gua destilada ou AZT por
10 dias. Os tecidos periodontais foram submetidos ?s an?lises morfom?trica, histopatol?gica
e imunohistoqu?mica para detec??o de MMP-2, MMP-9, RANKL, RANK, OPG e catepsina
K. N?veis de IL-1?, IL-10, TNF-? foram determinados por ELISA e n?veis de
mieloperoxidase(MPO) e glutationa(GSH) por espectrofotoscopia de UV vis?vel. O
tratamento com AZT 5 mg/kg reduziu o MPO (p <0.05) e IL-1? (p <0.05), elevou os n?veis
de IL-10 (p <0.05), reduziu a express?o de MMP-2, MMP-9, RANK, RANKL, catepsina K, e
elevou a express?o de OPG. Os achados revelam que a AZT na dosagem 5mg/kg apresenta
a??o anti-inflamat?ria na doen?a periodontal induzida por ligadura reduzindo a perda ?ssea
alveolar, diminuindo os n?veis de citocinas pr?-inflamat?rias e elevando a express?o de
agentes anti-inflamat?rios. / Periodontal diseases, highly prevalent disease in worldwide population, manifest
primarily in two distinct entities: plaque-induced gingivitis and periodontitis. Periodontitis is a
chronic inflammatory disease characterized of different levels of collagen, cementum, and
alveolar bone destruction. Recent experimental studies demonstrated anti-inflammatory and
antirreabsortive effect of antihypertensive agents of the angiotensin II receptor blockers class
on periodontal disease. The aim of this study was to evaluate the effects of azilsartan (AZT),
a potent inhibitor of the angiotensin II receptor which has minimal adverse effects on bone
loss, inflammation, and the expression of matrix metallo proteinases (MMPs), receptor
activator of nuclear factor kB ligand (RANKL), receptor activator of nuclear factor kB
(RANK), osteoprotegerin (OPG), cyclooxygenase-2 (COX-2), and cathepsin K in periodontal
tissue in a rat model of ligature-induced periodontitis. Male Wistar albino rats were randomly
divided into 5 groups of 20 rats each: (1) nonligated, water; (2) ligated, water; (3) ligated, 1
mg/kg AZT; (4) ligated, 5 mg/kg AZT; and (5) ligated, 10 mg/kg AZT. All groups were
treated with water or AZT for 10 days. Periodontal tissues were analyzed by morphometric
exam, histopathology and immunohistochemical detection of MMP-2, MMP-9, COX-2,
RANKL, RANK, OPG, and cathepsin K. Levels of IL-1b, IL-10, TNF-a, myeloperoxidase
(MPO), and glutathione (GSH) were determined by ELISA. Treatment with 5 mg/kg AZT
resulted in reduced MPO (p?0.05) and IL-1b (p?0.05) levels and increased in Il-10 levels
(p?0.05). It was observed a reduced expression of MMP-2, MMP-9, COX-2, RANK,
RANKL, cathepsin K, and a increased expression of OPG in the animals subjected to
experimental periodontitis and threated with AZT (5 mg/kg). Conclusions: These findings
suggest an anti-inflammatory and anti-reabsortive effects of AZT on ligature-induced
periodontitis in rats.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/19879 |
| Date | 19 March 2015 |
| Creators | Varela, Hugo de Almeida |
| Contributors | 83806059420, http://lattes.cnpq.br/3531154240424211, Ara?jo J?nior, Raimundo Fernandes de, 01847881459, http://lattes.cnpq.br/1903940945895093, Leit?o, Renata Ferreira de Carvalho, 43088139304, http://lattes.cnpq.br/5213035069793195, Ara?jo, Aurigena Antunes de |
| Publisher | Universidade Federal do Rio Grande do Norte, PROGRAMA DE P?S-GRADUA??O EM SA?DE COLETIVA, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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