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Previous issue date: 2011-08-05 / This laboratory study involves the participation of a group with professionals from
different areas that had contributed to the construction of a multidisciplinary
knowledge, about biological response of titanium surfaces modified through
thermochemical treatment by plasma. Thus, the crystalline phase was previously
characterized in relation to the topography, roughness, molhability and nitrogen
concentration in the samples surface. It s indispensable that materials implanted can
influence in a good cellular response as well as promotes a bacteria action. Surfaces
modified by plasma were exposed to different cultures such as: cellular (human
osteoblastic) and bacteria (Staphylococcus epidermidis ATCC35984 and
Pseudomonas aeruginosa ATCC 27853) in order to evaluate the biological response.
It was evaluated the adhesion, proliferation, morphology and cellular preference of
human ostheoblastic cells (HOST), as well as the formation of a biofilm and bacteria
proliferation. It was still analyzed the bacteria selectivity ability in relation to the
surfaces. The software Image Pro Plus was used to the counting of cells and bacteria
adhered to the surface of disks. The results were submitted to the variance analysis
(ANOVA), and then, by the Kruskal-Wallis test, using GraphPad Instat ? software,
version 3.5 to Windows. The nitrided samples in spite of show a higher roughness and molhability showed a smaller bacteria growing and higher cellular proliferation,
when compared to non treated samples, indicating that the treated material present a
high efficiency to biomedical implants / Este estudo laboratorial envolveu a participa??o de um grupo de profissionais de
?reas distintas, as quais contribu?ram na constru??o de um conhecimento
multidisciplinar, acerca da resposta biol?gica de superf?cies de tit?nio modificadas
por tratamento termoqu?mico a plasma. Para tanto, uma avalia??o da an?lise de fase
cristalina, topografia, rugosidade, molhabilidade e concentra??o de nitrog?nio nas
superf?cies das amostras foram previamente determinadas. ? imprescind?vel que
materiais implant?veis influenciem uma boa resposta celular como tamb?m
proporcionem uma a??o bacteriost?tica. Com o intuito de avaliar a resposta
biol?gica, superf?cies modificadas por plasma foram expostas as seguintes culturas:
celular (osteoblasto humano) e bacteriano (Staphylococcus epidermidis ATCC35984
e Pseudomonas aeruginosa ATCC 27853). Avaliou-se a ades?o, prolifera??o,
morfologia e prefer?ncia de c?lulas osteobl?sticas humanas (HOST) bem como a
forma??o de biofilme e prolifera??o bacteriana. Analisou-se, ainda, a capacidade de
seletividade de superf?cie pelas bact?rias. O software Image Pro Plus foi utilizado
para contagem das c?lulas e bact?rias aderidas ? superf?cie dos discos. Os
resultados foram submetidos ? an?lise de vari?ncia (ANOVA), seguido pelo teste de Kruskal-Wallis, utilizando o GraphPad Instat ?, vers?o 3.5 para Windows. As
amostras nitretadas apesar de apresentarem uma maior rugosidade e molhabilidade,
quando comparadas com as amostras n?o tratadas, tiveram um menor crescimento
bacteriano e uma maior prolifera??o celular. Indicando que o material tratado possui
uma alta efici?ncia para implantes biom?dicos
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/13351 |
| Date | 05 August 2011 |
| Creators | Aires, Michelle de Medeiros |
| Contributors | CPF:09621199468, http://lattes.cnpq.br/7441669258580942, Silva, Naisandra Bezerra da, CPF:91439760420, http://lattes.cnpq.br/6590909272236189, Fook, Marcus Vinicius Lia, CPF:00000000012, http://lattes.cnpq.br/4149843752530120, Alves J?nior, Clodomiro |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Ci?ncias da Sa?de, UFRN, BR, Ci?ncias da Sa?de |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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