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Previous issue date: 2005-10-11 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Rheumatoid arthritis (RA) is systemic auto imune disorder. It is caracterized by chronic inflammation of joints leading to progressive erosion of cartilage and bone. We investigated the effect of the administration of fucoidan, sulfated polysaccharides, from algae Fucus vesiculosus in the acute (6h) in zymosan-induced arthritis (AZy). Wistar rats (180-230 g) were used for all groups experimental. Non-treated animals received just intraarticular injection of 1 mg the zymosan, control group received intraarticular injection of 50 ?L the saline, groups received either fucoidan of Fucus vesiculosus (15, 30, 50 or 70 mg/Kg) or parecoxib (1 mg/Kg) 1 hour after injection of zymosan. After 6 h, the articular exudates were collected for evaluation of the cell influx and nitrite (Griess reaction) release. The sinovial membranes and articular cartilages were excised for histopathological analysis and by determination of the glycosaminoglycan (GAG), respectively. ZyA led to increased NO and cell influx into the joints. Therapeutic administration of the fucoidan or parecoxib did significantly inhibited the cell influx and the synovitis, as compared to non-treated rats (p<0,05), though being able to reduced NO release. Representative agarose gel electrophoresis of the GAGs, the content of condroitin-sulphate was observed during the process. These findings suggest that the fucoidan from Fucus vesiculosus has potential anti-inflammatory activity / A artrite reumat?ide (AR) ? uma doen?a auto-imune sist?mica, caracterizada por inflama??o cr?nica das articula??es, resultando em progressiva eros?o cartilaginosa e ?ssea. Neste trabalho foi investigado o efeito da administra??o do fucoidam, polissacar?deos sulfatados, da alga marinha Fucus vesiculosus na fase aguda (6h) da artrite induzida por zymosan (AZy). Ratos Wistar (180-230 g) foram utilizados para todos os grupos experimentais. Animais n?o tratados receberam apenas 1 mg de zymosan intraarticular (i.a), o grupo controle recebeu 50 ?L de solu??o salina intraarticular (i.a.), os outros grupos receberam fucoidam de Fucus vesiculosus (15, 30, 50 ou 70 mg/Kg,) ou parecoxib (1 mg/Kg), por via intraperitoneal (i.p.) 1 hora ap?s a inje??o de zymosan (i.a.). Ap?s 6 h, o exsudato articular foi coletado para an?lises do influxo celular e libera??o de nitrito (reagente de Griess). As membranas sinoviais e as cartilagens articulares foram retiradas para an?lises histopatol?gicas e para a determina??o dos glicosaminoglicanos, respectivamente. A AZy caracterizou-se por libera??o aumentada de NO e influxo de c?lulas inflamat?rias, nas juntas. A administra??o terap?utica do fucoidam ou parecoxib inibiu (p<0,05) o influxo celular, a libera??o de ?xido n?trico e a sinovite, comparado ao grupo n?o tratado. Por eletroforese em gel de agarose e tamp?o PDA 0,05M pH9,0 foi observado bandas com migra??es semelhantes ao condroitim sulfato (CS). Estes resultados sugerem que o fucoidam de Fucus vesiculosus tem um potencial anti-inflamat?rio
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/12624 |
| Date | 11 October 2005 |
| Creators | Xavier, Caroline Addison Carvalho |
| Contributors | CPF:06341462468, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783254U6&dataRevisao=null, Paiva, Patr?cia Maria Guedes, CPF:36565431434, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787593U2&dataRevisao=null, Rocha, Hugo Alexandre de Oliveira, CPF:76111830449, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799567J8&dataRevisao=null, Leite, Edda Lisboa |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Bioqu?mica, UFRN, BR, Bioqu?mica; Biologia Molecular |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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