The present study analyzes the capacity of collagen (coll)/sulfated glycosaminoglycan
(sGAG)-based surface coatings containing bioactive glass nanoparticles (BGN) in promoting the
osteogenic differentiation of human mesenchymal stroma cells (hMSC). Physicochemical characteristics
of these coatings and their effects on proliferation and osteogenic differentiation of hMSC
were investigated. BGN were stably incorporated into the artificial extracellular matrices (aECM).
Oscillatory rheology showed predominantly elastic, gel-like properties of the coatings. The complex
viscosity increased depending on the GAG component and was further elevated by adding BGN.
BGN-containing aECM showed a release of silicon ions as well as an uptake of calcium ions. hMSC
were able to proliferate on coll and coll/sGAG coatings, while cellular growth was delayed on aECM
containing BGN. However, a stimulating effect of BGN on ALP activity and calcium deposition
was shown. Furthermore, a synergistic effect of sGAG and BGN was found for some donors. Our
findings demonstrated the promising potential of aECM and BGN combinations in promoting bone
regeneration. Still, future work is required to further optimize the BGN/aECM combination for
increasing its combined osteogenic effect.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:89222 |
| Date | 24 January 2024 |
| Creators | Kroschwald, Lysann M., Allerdt, Felix, Bernhardt, Anne, Rother, Sandra, Zheng, Kai, Maqsood, Iram, Halfter, Norbert, Heinemann, Christiane, Möller, Stephanie, Schnabelrauch, Matthias, Hacker, Michael C., Rammelt, Stefan, Boccaccini, Aldo R., Hintze, Vera |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 12819 |
Page generated in 0.0021 seconds