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Previous issue date: 2015-05-29 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Entre os tipos mais incidentes de c?ncer no Brasil, o c?ncer cervical ? o quarto tipo mais comum entre as mulheres, com mais de 99,7% dos casos associados ? infec??o pelo Papilomasv?rus Humano (HPV), tipos 16 e 18 principalmente. A resposta limitada dos tumores malignos ? quimioterapia convencional levou ao desenvolvimento de novas estrat?gias farmacol?gicas baseadas no conhecimento de novos alvos terap?uticos. Considerando a diversidade dos escorpi?es e serpentes da fauna brasileira, esses animais pe?onhentos possuem um potencial farmacol?gico em seu veneno, sendo de grande interesse na pesquisa e desenvolvimento de novos f?rmacos a partir do veneno bruto ou fra??es. Desta forma, frente a esses venenos abre-se uma expectativa na busca de novos compostos com a??o terap?utica no tratamento do c?ncer. O objetivo deste trabalho foi avaliar o efeito citot?xico e apopt?tico do veneno bruto do escorpi?o Tityus serrulatus (TSR) e das serpentes Bothrops jararaca (BJ) e Bothrops erythromelas (BE) em linhagens de carcinoma cervical SiHa e HeLa, tranformadas pelos HPV 16 e 18, respectivamente. Em rela??o ? viabilidade celular pelo m?todo de MTT, obteve-se citotoxicidade nas linhagens tumorais desafiadas com os venenos das serpentes BJ e BE quando comparada ao veneno TSR. De forma similar, o processo de morte celular, avaliada por citometria de fluxo, foi superior quando desafiados com BJ e BE. Portanto, os venenos Bothrops demonstraram uma a??o citot?xica e apopt?tica nas linhagens estudadas, de maneira dose tempo-resposta dependentes. O presente trabalho sugere, pela primeira vez, um papel citot?xico e apopt?tico dos venenos das serpentes B. jararaca e B. erythromelas em linhagens de carcinoma cervical. / Among the types of cancer incidents in Brazil, cervical cancer is the fourth most common type among women, with more than 99.7% of cases associated with infection by human papillomaviruses (HPV) types 16 and 18 mainly. The limited of malignant tumors to conventional chemotherapy response led to the development of new pharmacological strategies based on knowledge of new therapeutic targets. Considering the diversity of scorpions and snakes of Brazilian fauna, these poisonous animals have a pharmacological potential in their venom, being of great interest in the new drugs research and development from raw or fractions poison. Thus, faced with these poisons opens an expectation in the search for new compounds with therapeutic action in the treatment of cancer. The objective of this study was to evaluate the cytotoxic effect and apoptosis of the poisons of the scorpion Tityus serrulatus (TSR), Bothrops jararaca (BJ) and Bothrops erythromelas (BE) in cervical carcinoma cell lines SiHa and HeLa, tranform by the respective HPV 16 and 18. In relation to cell viability by MTT method was obtained challenged cytotoxicity in tumor cell lines with the poisons of snakes BJ and BE compared to the TSR poisons. Similarly, the process of cell death was greater when challenged with BJ and BE. Therefore, Bothrops poisons showed a cytotoxic and apoptotic action on the lines studied, dose-dependent and time-response manner. This study suggests for the first time, a cytotoxic and apoptotic role of poisons of snakes B. jararaca and B. erythromelas in cervical carcinoma cell lines.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/21709 |
| Date | 29 May 2015 |
| Creators | Silva, Emanuelly Bernardes de Oliveira da |
| Contributors | 91430364491, Andrade Neto, Valter Ferreira de, 59626305487, Mendon?a, Tarsia Giabardo Silva, 32385349809, Farias, Kleber Juvenal Silva, Freitas, Janaina Cristiana de Oliveira Crispim |
| Publisher | PROGRAMA DE P?S-GRADUA??O EM CI?NCIAS FARMAC?UTICAS, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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